{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T17:19:02Z","timestamp":1793207942857,"version":"4.5.0"},"reference-count":24,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,5]]},"abstract":"<jats:p>There is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific for the 16S ribosomal RNA gene (<jats:italic>rrs<\/jats:italic>) of pathogenic and intermediate group <jats:italic>Leptospira<\/jats:italic> species. The lower limit of detection was 10 genomic equivalents\/reaction, and analytical specificity was high; we observed positive reactions for pathogenic\/intermediate groups and negative reactions for non-pathogenic <jats:italic>Leptospira<\/jats:italic> species and other bacterial species. We evaluated this assay in Thailand by using a case\u2013control study of 133 patients with laboratory-proven leptospirosis and 133 patients with other febrile illnesses. Using admission blood, we found that the <jats:italic>rrs<\/jats:italic> LAMP showed positive results in 58 of 133 cases (diagnostic sensitivity = 43.6, 95% confidence interval [CI] = 35.0\u201352.5) and in 22 of 133 controls (diagnostic specificity = 83.5, 95% CI = 76.0\u201389.3). Sensitivity was high for 39 patients who were culture positive for <jats:italic>Leptospira<\/jats:italic> spp. (84.6, 95% CI = 69.5\u201394.1). The <jats:italic>rrs<\/jats:italic> LAMP can provide an admission diagnosis in approximately half of patients with leptospirosis, but its clinical utility is reduced by a lower specificity.<\/jats:p>","DOI":"10.4269\/ajtmh.2011.10-0473","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T13:23:23Z","timestamp":1301664203000},"page":"614-620","source":"Crossref","is-referenced-by-count":53,"title":["Accuracy of Loop-Mediated Isothermal Amplification for Diagnosis of Human Leptospirosis in Thailand"],"prefix":"10.4269","volume":"84","author":[{"given":"Piengchan","family":"Sonthayanon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirongrong","family":"Chierakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janjira","family":"Thaipadungpanit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thareerat","family":"Kalambaheti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriphan","family":"Boonsilp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Premjit","family":"Amornchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee D.","family":"Smythe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Direk","family":"Limmathurotsakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P.","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Ann Trop Med Parasitol","first-page":"363","article-title":"Causes of acute, undifferentiated, febrile illness in rural Thailand: results of a prospective observational study","volume":"100","author":"Suttinont","year":"2006"},{"key":"R2","series-title":"J Infect Dis","first-page":"281","article-title":"Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of 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EMBNEW","volume":"4","author":"Nicholas","year":"1997"},{"key":"R18","series-title":"Am J Trop Med Hyg","first-page":"1099","article-title":"Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction","volume":"75","author":"Sonthayanon","year":"2006"},{"key":"R19","series-title":"J Clin Microbiol","first-page":"1363","article-title":"Optimization of culture of Leptospira from humans with leptospirosis","volume":"45","author":"Wuthiekanun","year":"2007"},{"key":"R20","series-title":"Int J Syst Bacteriol","first-page":"258","article-title":"International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Leptospira: Minutes of the Meeting, 6 to 10 August 1982, Boston, Massachusetts","volume":"34","author":"Stallman","year":"1984"},{"key":"R21","series-title":"PLoS Negl Trop Dis","first-page":"e56","article-title":"A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand","volume":"1","author":"Thaipadungpanit","year":"2007"},{"key":"R22","series-title":"J Clin Microbiol","first-page":"2521","article-title":"Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis","volume":"45","author":"Han","year":"2007"},{"key":"R23","series-title":"J Clin Microbiol","first-page":"2616","article-title":"Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples","volume":"41","author":"Iwamoto","year":"2003"},{"key":"R24","series-title":"BMC Infect Dis","first-page":"48","article-title":"Evaluation of surveillance case definition in the diagnosis of leptospirosis, using the microscopic agglutination test: a validation study","volume":"9","author":"Dassanayake","year":"2009"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/84\/4\/article-p614.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/84\/4\/article-p614.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/84\/4\/article-p614.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:57:24Z","timestamp":1723060644000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/84\/4\/article-p614.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4,5]]},"references-count":24,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2011.10-0473","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4,5]]}}}