{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:38:34Z","timestamp":1792798714364,"version":"4.5.0"},"reference-count":39,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,2,4]]},"abstract":"<jats:p>Soil-transmitted helminth infections remain a major public health burden in low- and middle-income countries. The traditional diagnosis by microscopic examination of fecal samples is insensitive and time-consuming. In this study, a pentaplex real-time polymerase chain reaction (PCR) was evaluated for the simultaneous detection of <jats:italic>Ancylostoma<\/jats:italic>, <jats:italic>Necator americanus<\/jats:italic>, <jats:italic>Ascaris lumbricoides<\/jats:italic>, and <jats:italic>Strongyloides stercoralis<\/jats:italic>. The results were compared with those obtained by conventional parasitological diagnostic methods. Real-time PCR was positive in 48 of 77 samples (62.3%) and microscopic examination was positive in six samples (7.8%) only (<jats:italic>P &lt;<\/jats:italic> 0.05). In conclusion, the real-time PCR assay described in this study provides a specific and sensitive diagnostic tool for the detection of these four helminth species in epidemiological studies and monitoring of treatment programs.<\/jats:p>","DOI":"10.4269\/ajtmh.2011.10-0499","type":"journal-article","created":{"date-parts":[[2011,2,3]],"date-time":"2011-02-03T17:02:07Z","timestamp":1296752527000},"page":"338-343","source":"Crossref","is-referenced-by-count":154,"title":["A Pentaplex Real-Time Polymerase Chain Reaction Assay for Detection of Four Species of Soil-Transmitted Helminths"],"prefix":"10.4269","volume":"84","author":[{"given":"Madihah","family":"Basuni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamail","family":"Muhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurulhasanah","family":"Othman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaco J.","family":"Verweij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maimunah","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorizan","family":"Miswan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anizah","family":"Rahumatullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhanah 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