{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:24:20Z","timestamp":1791030260257,"version":"4.1.0"},"reference-count":47,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,6,1]]},"abstract":"<jats:p>The technical capability of different methods to diagnose <jats:italic>Plasmodium<\/jats:italic> in maternal peripheral blood, placenta, and umbilical cord blood has not been assessed in Colombia and seldom explored in other malaria-endemic regions. We designed a study to compare the technical and the operational-economical performances of light microscopy (LM), nested polymerase chain reaction (nPCR), and histopathology (HP). In maternal blood, LM had 41% sensitivity and 100% specificity and in placental blood, 35% and 100%, respectively, compared with nPCR. In placental tissue, LM had 33% sensitivity and 95% specificity; and nPCR 47% and 77%, respectively; compared with HP. Light microscopy had the best operational-economical qualification. We concluded that nPCR and HP performed better compared with LM, but field implementation of these two techniques remains a problem. Therefore, LM is recommended as the gold standard for diagnosis of gestational malaria and placental blood infection in the field.<\/jats:p>","DOI":"10.4269\/ajtmh.2011.10-0507","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T18:44:22Z","timestamp":1306953862000},"page":"929-935","source":"Crossref","is-referenced-by-count":39,"title":["Diagnosis of Gestational, Congenital, and Placental Malaria in Colombia: Comparison of the Efficacy of Microscopy, Nested Polymerase Chain Reaction, and Histopathology"],"prefix":"10.4269","volume":"84","author":[{"given":"Iv\u00f3n M.","family":"Campos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary L.","family":"Uribe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Cuesta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Franco-Gallego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Carmona-Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Maestre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Diagn\u00f3stico de malaria","first-page":"39","author":"L\u00f3pez-Antu\u00f1ano","year":"1988"},{"key":"R2","series-title":"Ann Trop Med Parasitol","first-page":"419","article-title":"A review of practical techniques for the diagnosis of malaria","volume":"92","author":"Makler","year":"1998"},{"key":"R3","series-title":"PAHO Bull","first-page":"1","article-title":"Pan American Health Organization. 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