{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:58:27Z","timestamp":1791089907906,"version":"4.1.0"},"reference-count":32,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,3,1]]},"abstract":"<jats:p>A Western blot (WB) method using a lysate from <jats:italic>Trypanosoma cruzi<\/jats:italic> (Maracay strain) epimastigotes was evaluated. Serum samples from 37 patients with confirmed Chagas disease (cohort I), 27 Spanish patients with visceral leishmaniasis caused by <jats:italic>Leishmania infantum<\/jats:italic> (cohort II), and 28 Colombian patients with cutaneous leishmaniasis caused by <jats:italic>L. panamensis<\/jats:italic> and negative serology for Chagas disease (cohort III) were tested. The negative controls were 55 healthy seronegative subjects for <jats:italic>T. cruzi<\/jats:italic> and <jats:italic>Leishmania<\/jats:italic>; 28 of the negative controls were from a region endemic for Chagas disease and <jats:italic>Leishmania<\/jats:italic> (cohort IV), and 27 of the negative controls were from a non-endemic area for <jats:italic>Leishmania<\/jats:italic> and <jats:italic>T. cruzi<\/jats:italic> (cohort V). A homogeneous standard band pattern consisting of six antigenic bands corresponding to 28, 32, 38, 39, 40, and 48 kDa was recognized simultaneously for all Chagasic patients' sera. Sera from <jats:italic>Leishmania<\/jats:italic>-infected patients showed a heterogeneous band pattern that was easily differentiated from the pattern of patients with Chagas disease. WB with <jats:italic>T. cruzi<\/jats:italic> epimastigote antigen is an efficient method for diagnosis and may be used as an alternative to confirm <jats:italic>T. cruzi<\/jats:italic> and detect cross-reactivity with <jats:italic>Leishmania<\/jats:italic>.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.11-0111","type":"journal-article","created":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T16:17:19Z","timestamp":1331223439000},"page":"412-416","source":"Crossref","is-referenced-by-count":38,"title":["Identification of a Western Blot Pattern for the Specific Diagnosis of Trypanosoma cruzi Infection in Human Sera"],"prefix":"10.4269","volume":"86","author":[{"given":"Cristina","family":"Riera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireia","family":"Verges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Iniesta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roser","family":"Fisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"G\u00e1llego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Tebar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Port\u00fas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Reporte del grupo de trabajo cienti\u00ecfico sobre la enfermedad de Chagas","author":"World Health Organization","year":"2007"},{"key":"R2","series-title":"Mem Inst Oswaldo Cruz","first-page":"75","article-title":"Epidemiology of Chagas disease in non-endemic countries: the role of international migration","volume":"102","author":"Schmunis","year":"2007"},{"key":"R3","series-title":"Acta Trop","first-page":"14","article-title":"Chagas disease: a Latin American health problem becoming a world health problem","volume":"115","author":"Schmunis","year":"2010"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"1078","article-title":"Congenital transmission of Trypanosoma cruzi in Europe (Spain): a case report","volume":"75","author":"Riera","year":"2006"},{"key":"R5","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"798","article-title":"Chagas disease after organ transplantation\u2014Los Angeles, California, 2006","volume":"55","author":"Centers for Disease Control and Prevention (CDC)","year":"2006"},{"key":"R6","series-title":"Trans R Soc Trop Med Hyg","first-page":"1161","article-title":"Congenital Trypanosoma cruzi infection in a non-endemic area","volume":"101","author":"Mu\u00f1oz","year":"2007"},{"key":"R7","series-title":"Transfusion","first-page":"540","article-title":"Transfusion-acquired Trypanosoma cruzi infection","volume":"47","author":"Young","year":"2007"},{"key":"R8","series-title":"Cases J","first-page":"302","article-title":"Fatal congenital Chagas' disease in a non-endemic area: a case report","volume":"1","author":"Flores-Ch\u00e1vez","year":"2008"},{"key":"R9","series-title":"Transfusion","first-page":"1737","article-title":"Evaluation of a prototype Trypanosoma cruzi antibody assay with recombinant antigens on a fully automated chemiluminescence analyzer for blood donor screening","volume":"46","author":"Chang","year":"2006"},{"key":"R10","series-title":"Control of Chagas Disease. 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