{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:53:46Z","timestamp":1762271626103},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,4,1]]},"abstract":"<jats:p>Despite several drawbacks, rK39-based rapid immunochromatographic test is widely used for the diagnosis of visceral leishmaniasis (VL) in the Indian subcontinent. There is an urgent need to develop a better antigen. In this study we separated crude soluble antigens of <jats:italic>Leishmania donovani<\/jats:italic> by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hybridized with pool sera from pre- and post-treated VL patients, 6 months follow-up, endemic healthy (EHC), and nonendemic healthy controls (NEHC) by Western blotting. The sensitivity of enzyme-linked immunosorbent assay with identified protein was 95% (confidence interval [CI] = 89.6\u201398.01%), whereas the specificity for EHC, NEHC, and different disease groups were 96.3% (CI = 89.8\u201398.6%), 100% (CI = 95.8\u2013100%), and 97.4% (CI = 91.02\u201399.3%), respectively. This specific antigen was subjected to two-dimensional gel electrophoresis and after tryptic digestion, antigen was characterized by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry. Further analysis showed that it is a member of the heat shock protein family of 70 kDa, designated as BHUP1, and has great potential in the diagnosis of VL.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.11-0261","type":"journal-article","created":{"date-parts":[[2012,4,4]],"date-time":"2012-04-04T22:40:09Z","timestamp":1333579209000},"page":"601-605","source":"Crossref","is-referenced-by-count":13,"title":["Identification and Characterization of a Novel Leishmania donovani Antigen for Serodiagnosis of Visceral Leishmaniasis"],"prefix":"10.4269","volume":"86","author":[{"given":"Subodh","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinesh","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaya","family":"Chakravarty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhukar","family":"Rai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shyam","family":"Sundar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Trans R Soc Trop Med Hyg","first-page":"583","article-title":"A simple and economical direct agglutination test for serodiagnosis and sero-epidemiological studies of visceral 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leishmaniasis","volume":"63","year":"1998"}],"container-title":["The American Society of Tropical Medicine and 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