{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:12:41Z","timestamp":1780344761226,"version":"3.54.1"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,7,2]]},"abstract":"<jats:p>We evaluated the diagnostic accuracy of two broadly reactive rapid immunochromatographic tests (ICTs) for detection of IgM and IgG against <jats:italic>Orientia tsutsugamushi<\/jats:italic> by using archived acute-phase serum samples from 102 patients with laboratory-confirmed scrub typhus, and from 62 archived serum samples from patients with other causes of fever as a negative control. These ICTs were constructed by using a mixture of recombinant proteins: 1) C1, a chimeric protein containing epitopes of the 56-kD antigen from Karp and TA763 strains; 2) Ktr56; and 3) Gmr56. Sensitivities of the ICTs for detection of IgM and IgG were 90.2% (95% confidence interval [CI] = 84.4\u201396.0%) and 86.3% (95% CI = 80.9\u201393.8%), respectively. Specificities were 85.5% (95% CI = 73.9\u201392.2%) and 96.8% (95% CI = 90.3\u2013100%), respectively. Both assays were more sensitive and specific than the standard immune immunofluorescence assay for the early diagnosis of scrub typhus.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.11-0583","type":"journal-article","created":{"date-parts":[[2012,7,4]],"date-time":"2012-07-04T20:56:49Z","timestamp":1341435409000},"page":"148-152","source":"Crossref","is-referenced-by-count":22,"title":["Development of New, Broadly Reactive, Rapid IgG and IgM Lateral Flow Assays for Diagnosis of Scrub Typhus"],"prefix":"10.4269","volume":"87","author":[{"given":"Saowaluk","family":"Silpasakorn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nujorn","family":"Srisamut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pattama","family":"Ekpo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Chung","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Mei","family":"Ching","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupin","family":"Suputtamongkol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Curr 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typhus by indirect immunofluorescence","volume":"112","author":"Bozeman","year":"1963"},{"key":"R11","series-title":"Am J Trop Med","first-page":"967","article-title":"Analysis of the cross-reactivity of various 56 kDa recombinant protein antigens with serum samples collected after Orientia tsutsugamushi infection by ELISA","volume":"84","author":"Chao","year":"2011"},{"key":"R12","series-title":"Ann N Y Acad Sci","first-page":"375","article-title":"An improved method for the purification and refolding of r56-kDa proteins from Gilliam and Kato strains of Orientia tsutsugamushi","volume":"990","author":"Yang","year":"2003"},{"key":"R13","series-title":"Manufacturing High-Quality Gold Sol: Understanding Key Engineering Aspects of the Production of Colloidal Gold Can Optimize the Quality and Stability of Gold Labeling Components","author":"Chaudhuri","year":"2001"},{"key":"R14","series-title":"Plos Nleg Trop Med","first-page":"e610","article-title":"Strategies for diagnosis and treatment of suspected leptospirosis: a cost-benefit analysis","volume":"4","author":"Suputtamongkol","year":"2010"},{"key":"R15","series-title":"Am J Trop Med","first-page":"532","article-title":"Humoral and cellular responses in scrub typhus patients reflecting primary infection and reinfection with Rickettsia tsutsugamushi","volume":"31","author":"Bourgeois","year":"1982"},{"key":"R16","series-title":"Clin Infect Dis","first-page":"S203","article-title":"Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientia tsutsugamushi","volume":"48","author":"Kelly","year":"2009"},{"key":"R17","series-title":"Jpn J Med Sci Biol","first-page":"337","article-title":"Antigenic analysis by direct immunofluorescence of 114 isolates of Rickettsia tsutsugamushi recovered from febrile patients in rural Malaysia","volume":"32","author":"Shirai","year":"1979"},{"key":"R18","series-title":"Southeast Asian J Trop Med Public Health","first-page":"557","article-title":"Antigenic types of Orientia tsutsugamushi in Malaysia","volume":"33","author":"Tay","year":"2002"}],"container-title":["The American Society of Tropical Medicine and 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