{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:26:20Z","timestamp":1775694380828,"version":"3.50.1"},"reference-count":6,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,1]]},"abstract":"<jats:p>The rapid analyte measurement platform (RAMP) system is an immunoassay test for West Nile virus (WNV) detection. Although reverse transcriptase-polymerase chain reaction (RT-PCR) methodology has been regarded as the gold standard for confirming WNV presence, usage of RAMP testing kits has increased in the past years. We collected RAMP test result data that were subsequently confirmed with RT-PCR methodology from mosquito control agencies to evaluate the efficacy of the RAMP testing. Results indicate that there are a high number of false positives (RAMP positive, RT-PCR negative) with RAMP testing. Correlation between RAMP unit values and RT-PCR cycle threshold values were varied depending on the primer\/probe being compared. Comparison of RT-PCR results (on the same samples) between laboratories also indicates variation among the procedures and their potential to influence the RAMP testing efficacies. We discuss the potential issues and solutions that could prevent the high rate of false positives.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.11-0662","type":"journal-article","created":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T21:56:16Z","timestamp":1343858176000},"page":"359-363","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of a Rapid Analyte Measurement Platform for West Nile Virus Detection Based on United States Mosquito Control Programs"],"prefix":"10.4269","volume":"87","author":[{"given":"Banugopan","family":"Kesavaraju","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ary","family":"Farajollahi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard L.","family":"Lampman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Hutchinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina M.","family":"Krasavin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonya E.","family":"Graves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sammie L.","family":"Dickson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Annu Rev Med","first-page":"181","article-title":"West Nile Virus: epidemiology and clinical features of an emerging epidemic in the United States","volume":"57","author":"Hayes","year":"2006"},{"key":"R2","series-title":"J Am Mosq Control Assoc","first-page":"64","article-title":"Evaluation of commercial assays for detecting West Nile virus antigen","volume":"22","author":"Burkhalter","year":"2006"},{"key":"R3","series-title":"J Vector Ecol","first-page":"324","article-title":"Comparative field analyses of rapid analyte measurement platform and reverse transcriptase polymerase chain reaction assays for West Nile virus surveillance","volume":"34","author":"Williges","year":"2009"},{"key":"R4","series-title":"Virology","first-page":"190","article-title":"Limited evolution of West Nile virus has occurred during its southwesterly spread in the United States","volume":"309","author":"Beasley","year":"2003"},{"key":"R5","series-title":"J Clin Microbiol","first-page":"4066","article-title":"Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR Assay","volume":"38","author":"Lanciotti","year":"2000"},{"key":"R6","series-title":"J Am Mosq Control Assoc","first-page":"389","article-title":"Detection of West Nile virus in large pools of mosquitoes","volume":"23","author":"Sutherland","year":"2007"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/87\/2\/article-p359.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/87\/2\/article-p359.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/87\/2\/article-p359.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T17:32:30Z","timestamp":1723051950000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/87\/2\/article-p359.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,1]]},"references-count":6,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2012.11-0662","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,1]]}}}