{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:23:23Z","timestamp":1790717003542,"version":"4.1.0"},"reference-count":17,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,2,6]]},"abstract":"<jats:p>\n                    We previously described loop-mediated isothermal amplification (LAMP) for detection of\n                    <jats:italic>Schistosoma haematobium<\/jats:italic>\n                    and\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    DNA in infected snails. In the present study, we adapted the LAMP assay for application in field laboratories in schistosomiasis-endemic areas. Isolation of DNA was simplified by blotting snail tissue (extracted in NaOH\/sodium dodecyl sulfate) onto treated membranes, which enabled preservation at ambient temperatures. A ready-mix of LAMP reagents, suitable for shipment at ambient temperature and storage in minimal refrigeration, was used. Local survey teams without experience in molecular biology acquired operational expertise with this test within a few hours. Fifty-four field-caught snails were tested locally by LAMP and 59 were tested at similar conditions in Jerusalem. The LAMP results were consistent with those of a polymerase chain reaction; only four samples showed false-negative results. Results indicate that LAMP assays are suitable for detection of\n                    <jats:italic>S. haematobium<\/jats:italic>\n                    and\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    in low-technology parasitology laboratories in which schistosomiasis elimination activities are undertaken.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.2012.12-0208","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T10:51:11Z","timestamp":1354618271000},"page":"344-351","source":"Crossref","is-referenced-by-count":82,"title":["Evaluation of Loop-Mediated Isothermal Amplification Suitable for Molecular Monitoring of Schistosome-Infected Snails in Field Laboratories"],"prefix":"10.4269","volume":"88","author":[{"given":"Joseph","family":"Hamburger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Abbasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Curtis","family":"Kariuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsabina","family":"Wanjala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elton","family":"Mzungu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Mungai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Muchiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles H.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Trop Med Int Health","first-page":"1475","article-title":"China's new strategy to block Schistosoma japonicum transmission: experiences and impact beyond schistosomiasis","volume":"14","author":"Wang","year":"2009"},{"key":"R2","series-title":"Int J Parasitol","first-page":"1243","article-title":"The challenge of effective surveillance in moving from low transmission to elimination of schistosomiasis in China","volume":"41","author":"Spear","year":"2011"},{"key":"R3","series-title":"Am J Trop Med Hyg","first-page":"765","article-title":"Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection","volume":"71","author":"Hamburger","year":"2004"},{"key":"R4","series-title":"Trends Parasitol","first-page":"575","article-title":"Transmission control for schistosomiasis\u2014why it matters now","volume":"22","author":"King","year":"2006"},{"key":"R5","series-title":"Am J Trop Med Hyg","first-page":"872","article-title":"A polymerase chain reaction assay for detecting snails infected with bilharzias parasites (Schistosoma mansoni) from very early prepatency","volume":"59","author":"Hamburger","year":"1998"},{"key":"R6","series-title":"Am J Trop Med Hyg","first-page":"907","article-title":"Polymerase chain reaction assay bnased on highly repeated sesequence of Schistosoma haematobium: a potential tool for monitoring schistosome infested water","volume":"65","author":"Hamburger","year":"2001"},{"key":"R7","series-title":"Am J Trop Med Hyg","first-page":"427","article-title":"Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency","volume":"83","author":"Abbasi","year":"2010"},{"key":"R8","series-title":"Am J Trop Med Hyg","first-page":"542","article-title":"Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis by PCR and loop-mediated isothermal amplification (LAMP) assay","volume":"83","author":"Kumagai","year":"2010"},{"key":"R9","series-title":"Int J Parasitol","first-page":"327","article-title":"Sensitive and rapid detection of Schistosoma japonicum DNA by loop-mediated isothermal amplification (LAMP)","volume":"40","author":"Xu","year":"2010"},{"key":"R10","series-title":"Third STAG Global Working Group Meeting on Monitoring and Evaluation of Preventive Chemotherapy. Summary Points and Recommendations","author":"World Health Organization","year":"2012"},{"key":"R11","series-title":"J Helminthol","first-page":"107","article-title":"Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. bovis","volume":"84","author":"Webster","year":"2010"},{"key":"R12","series-title":"Acta Trop","article-title":"Detection and quantification of schistosome DNA in freshwater snails using either fluorescent probes in real-time PCR or oligochromatographic dipstick assays targeting the ribosomal intergenic spacer","author":"Kane","year":"2011"},{"key":"R13","series-title":"Am J Trop Med Hyg","first-page":"950","article-title":"Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence","volume":"76","author":"Abbasi","year":"2007"},{"key":"R14","series-title":"J Biochem Biophys Methods","first-page":"145","article-title":"Real-time turbidimetry of LAMP reaction for quantifying template DNA","volume":"59","author":"Mori","year":"2004"},{"key":"R15","series-title":"Eur J Microbiol Infect Dis","first-page":"937","article-title":"Use of loop mediated isothermal amplification of DNA for the rapid detection of Micobacterium tuberculosis in clinical cases","volume":"30","author":"Neonakis","year":"2011"},{"key":"R16","series-title":"Am J Trop Med Hyg","first-page":"83","article-title":"Factors affecting infection or reinfection with Schistosoma haematobium in coastal Kenya: Survival analysis during a nine-year school-based treatment","volume":"75","author":"Satayathum","year":"2006"},{"key":"R17","series-title":"Am J Trop Med Hyg","first-page":"1034","article-title":"Spatial and temporal variations in local transmission of Schistosoma haematobium in Msambweni, Kenya","volume":"75","author":"Clennon","year":"2006"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/2\/article-p344.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/2\/article-p344.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/2\/article-p344.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T14:43:18Z","timestamp":1723041798000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/2\/article-p344.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,6]]},"references-count":17,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2012.12-0208","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,6]]}}}