{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:10:02Z","timestamp":1723054202237},"reference-count":37,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,7]]},"abstract":"<jats:p>Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of <jats:italic>Gnathostoma binucleatum<\/jats:italic> and in Asia mainly by <jats:italic>G. spinigerum<\/jats:italic>. The availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. In this study, six immunodominant peptides were identified and characterized from <jats:italic>G. binucleatum<\/jats:italic>, somatic antigens (AgS: 24, 32, and 40 kDa) and excretory-secretory antigens (AgES: 42, 44, and 56 kDa) by two-dimensional immunoblot analysis. Among those immunodominant peptides, two AgS spots were characterized by mass spectrometric analysis (32 kDa; pI 6.3 and 6.5) and identified as type 1 galectins. In accordance with this finding, a fraction of AgS exhibited affinity to lactose and displayed a 100% specificity and sensitivity for the diagnosis of human gnathostomiasis.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.12-0233","type":"journal-article","created":{"date-parts":[[2012,9,21]],"date-time":"2012-09-21T15:15:22Z","timestamp":1348240522000},"page":"888-896","source":"Crossref","is-referenced-by-count":7,"title":["Identification of Immunodominant Peptides from Gnathostoma binucleatum"],"prefix":"10.4269","volume":"87","author":[{"given":"Samuel","family":"Campista-Le\u00f3n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Delgado-Vargas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham","family":"Landa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaethe","family":"Willms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector Samuel","family":"L\u00f3pez-Moreno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Mendoza-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"R\u00edos-Sicairos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngel Noel","family":"Boj\u00f3rquez-Contreras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia P\u00e1z","family":"D\u00edaz-Camacho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"J Parasitol","first-page":"537","article-title":"ITS-2 rDNA sequencing of Gnathostoma species (Nematoda) and elucidation of the species causing human gnathostomiasis in the Americas","volume":"86","author":"Almeyda-Artigas","year":"2000"},{"key":"R2","series-title":"Revista de la Facultad de Medicina de la UNAM","first-page":"192","article-title":"Gnathostomosis","volume":"43","author":"D\u00edaz-Camacho","year":"2000"},{"key":"R3","series-title":"Ann Trop Med Parasitol","first-page":"629","article-title":"Evaluation of the antigenic similarities of adult-worm extracts from three Gnathostoma species, using sera from Mexican and Japanese patients with Gnathostoma infections","volume":"97","author":"Ishiwata","year":"2003"},{"key":"R4","series-title":"Southeast Asian J Trop Med Public Health","first-page":"444","article-title":"A study on immunodiagnosis of gnathostomiasis by ELISA and double diffusion with special reference to the antigenicity of Gnathostoma doloresi","volume":"18","author":"Tada","year":"1987"},{"key":"R5","series-title":"World Class Parasites: Food-Borne Parasitic Zoonoses. 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