{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:15:21Z","timestamp":1790072121076,"version":"4.0.1"},"reference-count":14,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,5]]},"abstract":"<jats:p>In schistosomiasis elimination programs, successful discrimination of <jats:italic>Schistosoma haematobium<\/jats:italic> from the related animal <jats:italic>Schistosoma<\/jats:italic> parasites will be essential for accurate detection of human parasite transmission. Polymerase chain reaction assays employing primers from two newly selected repeated sequences, named Sh73 and Sh77, did not discriminate <jats:italic>S. haematobium<\/jats:italic> when amplifying Sh73-77 intra- or inter-repeats. However, amplification between Sh73 and the previously described DraI repeat exhibited discriminative banding patterns for <jats:italic>S. haematobium<\/jats:italic> and <jats:italic>Schistosoma bovis<\/jats:italic> (sensitivity 1 pg and 10 pg, respectively). It also enabled banding pattern discrimination of <jats:italic>Schistosoma curassoni<\/jats:italic> and <jats:italic>Schistosoma intercalatum<\/jats:italic>, but <jats:italic>Schistosoma mattheei<\/jats:italic> and <jats:italic>Schistosoma margrebowiei<\/jats:italic> did not yield amplicons. Similar inter-repeat amplification between Sh77 and DraI yielded amplicons with discriminative banding for <jats:italic>S. haematobium<\/jats:italic>, and <jats:italic>S. bovis<\/jats:italic>; however, <jats:italic>S. mattheei<\/jats:italic> was detected only at low sensitivity (1 ng). The Sh73\/DraI assay detected snails infected with <jats:italic>S. haematobium<\/jats:italic>, <jats:italic>S. bovis<\/jats:italic>, or both, and should prove useful for screening snails where discrimination of <jats:italic>S. haematobium<\/jats:italic> from related schistosomes is required.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.12-0243","type":"journal-article","created":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T05:46:07Z","timestamp":1351575967000},"page":"1059-1064","source":"Crossref","is-referenced-by-count":12,"title":["Differentiating Schistosoma haematobium from Related Animal Schistosomes by PCR Amplifying Inter-Repeat Sequences Flanking Newly Selected Repeated Sequences"],"prefix":"10.4269","volume":"87","author":[{"given":"Ibrahim","family":"Abbasi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Hamburger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Curtis","family":"Kariuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.","family":"Mungai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric M.","family":"Muchiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles H.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"World Health Organ Tech Rep Ser","first-page":"2","article-title":"Prevention and control of 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