{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:36:03Z","timestamp":1791304563713,"version":"4.3.1"},"reference-count":7,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,9]]},"abstract":"<jats:p>Culture-independent molecular characterization of infecting <jats:italic>Leptospira<\/jats:italic> human blood specimens from a 2008 outbreak of human leptospirosis in central Sri Lanka was carried out. Of 58 quantitative real-time polymerase chain reaction-positive samples analyzed for seven multi-locus sequence typing (MLST) housekeeping genes (<jats:italic>mreA<\/jats:italic>, <jats:italic>pfkB<\/jats:italic>, <jats:italic>pntA<\/jats:italic>, <jats:italic>sucA<\/jats:italic>, <jats:italic>tpiA<\/jats:italic>, <jats:italic>fadD<\/jats:italic>, and <jats:italic>glmU<\/jats:italic>), interpretable data was obtained from 12 samples. Mean bacterial load was 2.2 \u00d7 10<jats:sup>5<\/jats:sup> among specimens with complete MLST profiles compared with 1.3 \u00d7 10<jats:sup>4<\/jats:sup> among specimens without complete MLST profiles; all specimens with complete profiles had at least 4.9 \u00d7 10<jats:sup>4<\/jats:sup> <jats:italic>Leptospira<\/jats:italic>\/mL (<jats:italic>t<\/jats:italic> = 5, <jats:italic>P<\/jats:italic> &lt; 0.001). Most (11\/12) identified sequence types were ST1 (<jats:italic>L. interrogans<\/jats:italic> serovar Lai) and ST44 (<jats:italic>L. interrogans<\/jats:italic> serovar Geyaweera). MLST can be used to directly identify infecting <jats:italic>Leptospira<\/jats:italic> strains in blood samples obtained during acute illness without the need for culture isolation, but it shows important limitations related to bacterial load.<\/jats:p>","DOI":"10.4269\/ajtmh.2012.12-0526","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T15:51:11Z","timestamp":1354636271000},"page":"184-185","source":"Crossref","is-referenced-by-count":31,"title":["Utility and Limitations of Direct Multi-Locus Sequence Typing on qPCR-Positive Blood to Determine Infecting Leptospira Strain"],"prefix":"10.4269","volume":"88","author":[{"given":"Suneth B.","family":"Agampodi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo C.","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph M.","family":"Vinetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Matthias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"PLoS Negl Trop Dis","first-page":"e120","article-title":"Sequence-based typing of Leptospira: epidemiology in the genomic era","volume":"1","author":"Levett","year":"2007"},{"key":"R2","series-title":"Ann Clin Microbiol Antimicrob","first-page":"28","article-title":"Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species","volume":"5","author":"Ahmed","year":"2006"},{"key":"R3","series-title":"PLoS Negl Trop Dis","first-page":"e56","article-title":"A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand","volume":"1","author":"Thaipadungpanit","year":"2007"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"471","article-title":"Leptospirosis outbreak in Sri Lanka in 2008: lessons for assessing the global burden of disease","volume":"85","author":"Agampodi","year":"2011"},{"key":"R5","series-title":"Geneious Pro 5.4.6","author":"Drummond","year":"2011"},{"key":"R6","series-title":"Nature","first-page":"888","article-title":"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing","volume":"422","author":"Ren","year":"2003"},{"key":"R7","series-title":"Indian J Med Res","first-page":"135","article-title":"Lai-like Leptospira from the Andaman Islands","volume":"112","author":"Sehgal","year":"2000"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/1\/article-p184.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/1\/article-p184.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/1\/article-p184.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:47:57Z","timestamp":1723056477000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/1\/article-p184.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,9]]},"references-count":7,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2012.12-0526","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,9]]}}}