{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:58:19Z","timestamp":1790315899920,"version":"4.1.0"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Standard diagnostics for <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MTB) including acid-fast bacilli (AFB) smear and culture, and Xpert<jats:sup>\u2122<\/jats:sup> MTB\/RIF real-time Polymerase Chain Reaction (RT-PCR; Xpert) have variable sensitivity and\/or long turnaround times. We describe the clinical performance of a laboratory-developed tissue-based MTB PCR compared with AFB culture and Xpert using a composite reference standard (CRS). Over an 8-year period, MTB PCR was performed on pulmonary, pleural, or lymph node specimens for 36 patients. Of these, 11 met criteria for confirmed\/probable MTB using CRS. MTB PCR was positive in 100% (11\/11), AFB cultures were positive in 73% (8\/11), and Xpert in 0% (0\/4). MTB PCR was negative in 25 cases of \u201cNo MTB\u201d (100% specific). The MTB PCR assay resulted faster than positive AFB culture (mean time 4.3 versus 21.2 days). Tissue-based MTB PCR was associated with increased and rapid detection of MTB, improving clinical sensitivity in strongly suspected MTB cases.<\/jats:p>","DOI":"10.4269\/ajtmh.21-0104","type":"journal-article","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T14:21:01Z","timestamp":1632147661000},"page":"1657-1661","source":"Crossref","is-referenced-by-count":1,"title":["Increased Detection of Mycobacterium tuberculosis Disease Using a Tissue-Based Laboratory-Developed Polymerase Chain Reaction Assay Compared to Standard Diagnostics"],"prefix":"10.4269","volume":"105","author":[{"given":"Natalie A.","family":"Mackow","sequence":"first","affiliation":[{"name":"1Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Abi-Raad","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Yale University School of Medicine, New Haven, Connecticut;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A.","family":"Kerantzas","sequence":"additional","affiliation":[{"name":"3Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Hui","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Yale University School of Medicine, New Haven, Connecticut;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maricar","family":"Malinis","sequence":"additional","affiliation":[{"name":"4Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwan M.","family":"Azar","sequence":"additional","affiliation":[{"name":"4Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/6\/article-p1657.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/105\/6\/article-p1657.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T23:01:36Z","timestamp":1638399696000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/6\/article-p1657.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,1]]},"references-count":0,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.21-0104","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,1]]}}}