{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:10:47Z","timestamp":1777435847269,"version":"3.51.4"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p><jats:italic>Plasmodium malariae<\/jats:italic> infections are often asymptomatic and long-lasting. Mixed infections are often underdetected in areas where <jats:italic>P. malariae<\/jats:italic>, <jats:italic>P. vivax<\/jats:italic>, and <jats:italic>P. falciparum<\/jats:italic> are coendemic. In this study, we described the occurrence of these species circulating as single or mixed infections in Par\u00e1 state, Brazil, in the Amazon region, with the purpose of clarifying the impact of misidentification of parasite species based only on morphological description using thick blood smear. By using real-time polymerase chain reaction based on the amplification of the mitochondrial DNA, we detected a prevalence of 46% (58\/126) mixed infections with 33.3% <jats:italic>P. malariae\/P. vivax<\/jats:italic> which were read as <jats:italic>P. vivax<\/jats:italic> monoinfections by microscopy detection. Our findings confirmed the high circulation of <jats:italic>P. malariae<\/jats:italic> in a malaria endemic area in the Brazilian Amazon region.<\/jats:p>","DOI":"10.4269\/ajtmh.21-0296","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T12:06:18Z","timestamp":1628510778000},"page":"1184-1186","source":"Crossref","is-referenced-by-count":5,"title":["Mixed Plasmodium malariae Infections Were Underdetected in a Malaria Endemic Area in the Amazon Region, Brazil"],"prefix":"10.4269","volume":"105","author":[{"given":"Maristela G.","family":"Cunha","sequence":"first","affiliation":[{"name":"1Laborat\u00f3rio de Microbiologia e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille S.","family":"Santos","sequence":"additional","affiliation":[{"name":"2Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milene","family":"Raiol","sequence":"additional","affiliation":[{"name":"2Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheyla Patr\u00edcia T.","family":"Costa","sequence":"additional","affiliation":[{"name":"1Laborat\u00f3rio de Microbiologia e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Maria R.","family":"Ventura","sequence":"additional","affiliation":[{"name":"3Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marinete M.","family":"P\u00f3voa","sequence":"additional","affiliation":[{"name":"3Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Ananindeua, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c2ndrea","family":"Ribeiro-dos-Santos","sequence":"additional","affiliation":[{"name":"2Laborat\u00f3rio de Gen\u00e9tica Humana e M\u00e9dica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m, Par\u00e1, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/5\/article-p1184.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/105\/5\/article-p1184.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T14:32:54Z","timestamp":1635949974000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/5\/article-p1184.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.21-0296","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]}}}