{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:10:27Z","timestamp":1760965827610,"version":"build-2065373602"},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,9]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Highly sensitive molecular techniques for the detection of low-level <jats:italic>Plasmodium falciparum<\/jats:italic> parasitemia are highly useful for various clinical and epidemiological studies. However, differences in how blood samples are preserved, the quantity of blood stored, as well as genomic DNA extraction methods used may compromise the potential usefulness of these methodologies. This study compared diagnostic sensitivity based on microscopy and malaria rapid diagnostic tests (mRDTs), with quantitative polymerase chain reaction (qPCR) <jats:italic>P. falciparum<\/jats:italic> positivity of dried blood spots (DBS) or whole blood pellets (WBP) from pregnant women using different DNA extraction protocols (Chelex-saponin or a commercial kit). Samples from 129 pregnant women were analyzed, of which 13 were <jats:italic>P. falciparum<\/jats:italic> positive by mRDT and 5 by microscopy. By using extraction kit on WBP and on DBS, qPCR positivity was 27 (20.9%) and 16 (12.4%), respectively, whereas Chelex extraction on DBS only resulted in 4 (3.1%) <jats:italic>P. falciparum<\/jats:italic> positive samples. Thus, extraction using commercial kits greatly improve the likelihood of detecting <jats:italic>P. falciparum<\/jats:italic> infections.<\/jats:p>","DOI":"10.4269\/ajtmh.21-0496","type":"journal-article","created":{"date-parts":[[2021,12,6]],"date-time":"2021-12-06T15:32:16Z","timestamp":1638804736000},"page":"846-849","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods"],"prefix":"10.4269","volume":"106","author":[{"given":"Queen","family":"Saidi","sequence":"first","affiliation":[{"name":"Kilimanjaro Clinical Research Institute, Moshi, Tanzania;"},{"name":"Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark; Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Minja","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Tanga Research Centre, Tanga, Tanzania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Njau","sequence":"additional","affiliation":[{"name":"Kilimanjaro Clinical Research Institute, Moshi, Tanzania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helle","family":"Hansson","sequence":"additional","affiliation":[{"name":"Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark; Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reginald","family":"Kavishe","sequence":"additional","affiliation":[{"name":"Kilimanjaro Clinical Research Institute, Moshi, Tanzania;"},{"name":"Kilimanjaro Christian Medical University College, Moshi, Tanzania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Alifrangis","sequence":"additional","affiliation":[{"name":"Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark; Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Expert Rev Anti Infect Ther","first-page":"1177","article-title":"Diagnosing malaria in pregnancy: an update","volume":"10:","year":"2012"},{"key":"b2","series-title":"BMC Pregnancy Childbirth","first-page":"108","article-title":"Uptake of intermittent preventive treatment for malaria during pregnancy with sulphadoxine-pyrimethamine (IPTp-SP) among postpartum women in Zomba District, Malawi: a cross-sectional 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