{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T21:01:08Z","timestamp":1778706068166,"version":"3.51.4"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Transovarial transmission (TOT) of dengue virus (DENV) in <jats:italic>Aedes <\/jats:italic>spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild <jats:italic>Aedes albopictus<\/jats:italic> populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of <jats:italic>Ae. albopictus<\/jats:italic> each containing 30 larvae were processed for DENV detection by using conventional reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of <jats:italic>Ae. albopictus<\/jats:italic>, and this suggests that this species may be an important vector of DENV in Cuba.<\/jats:p>","DOI":"10.4269\/ajtmh.21-0710","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T14:14:59Z","timestamp":1636380899000},"page":"582-584","source":"Crossref","is-referenced-by-count":6,"title":["First Record of Natural Transovarial Transmission of Dengue Virus in Aedes albopictus from Cuba"],"prefix":"10.4269","volume":"106","author":[{"given":"Luis Augusto","family":"Piedra","sequence":"first","affiliation":[{"name":"1Vector Control Department, Institute of Tropical Medicine \u201cPedro Kour\u00ed,\u201d Havana, Cuba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liss Claudia","family":"Martinez","sequence":"additional","affiliation":[{"name":"1Vector Control Department, Institute of Tropical Medicine \u201cPedro Kour\u00ed,\u201d Havana, Cuba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Ruiz","sequence":"additional","affiliation":[{"name":"1Vector Control Department, Institute of Tropical Medicine \u201cPedro Kour\u00ed,\u201d Havana, Cuba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Ramon","family":"Vazquez","sequence":"additional","affiliation":[{"name":"2Faculty of Health Sciences, Havana, Cuba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Guadalupe","family":"Guzman","sequence":"additional","affiliation":[{"name":"3Department of Virology, Institute of Tropical Medicine \u201cPedro Kour\u00ed,\u201d Havana, Cuba;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Rey","sequence":"additional","affiliation":[{"name":"4Florida Medical Entomology Laboratory, University of Florida, Gainesville, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Andres","family":"Bisset","sequence":"additional","affiliation":[{"name":"1Vector Control Department, Institute of Tropical Medicine \u201cPedro Kour\u00ed,\u201d Havana, Cuba;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/2\/article-p582.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/106\/2\/article-p582.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T14:58:58Z","timestamp":1643813938000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/2\/article-p582.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,2]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.21-0710","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,2]]}}}