{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T13:10:41Z","timestamp":1760965841430,"version":"build-2065373602"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,9]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Successful completion of the dengue virus (DENV) life cycle in its mosquito vectors is important for efficient human\u2013mosquito\u2013human cycle of transmission, but the virus\u2013mosquito interactions that underpin this critical event are poorly defined. To understand the virus\u2013host interactions that determine viral infection by <jats:italic>Aedes aegypti<\/jats:italic>, the principal DENV vector, the authors compared transcriptomic changes in the head\/thorax of the mosquito after intrathoracic infection with the wild-type DENV2 16681 strain and its attenuated derivative, PDK53. Using high-throughput RNA-sequencing, the authors identified 1,629 differentially expressed genes (DEGs) during 16681 infection, compared with only 22 DEGs identified during PDK53 infection, indicating that 16681 infection triggers a more robust host transcriptomic response compared with PDK53 infection. The authors further found that 16681 infection, but not PDK53 infection, altered metabolism in these heads\/thoraces. Altogether, our findings reveal differential regulation of metabolic processes during wild-type and attenuated DENV infection, and suggest the need for future work to study the role of metabolic processes in determining DENV infection and replication in its mosquito vectors.<\/jats:p>","DOI":"10.4269\/ajtmh.21-0734","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T07:43:39Z","timestamp":1641800619000},"page":"900-904","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic Processes Are Differentially Regulated During Wild-Type and Attenuated Dengue Virus Infection in Aedes aegypti"],"prefix":"10.4269","volume":"106","author":[{"given":"Tanamas","family":"Siriphanitchakorn","sequence":"first","affiliation":[{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore;"},{"name":"Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassandra M.","family":"Modahl","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Manjunatha","family":"Kini","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore;"},{"name":"Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eng Eong","family":"Ooi","sequence":"additional","affiliation":[{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore;"},{"name":"Saw Swee Hock School of Public Health, National University of Singapore, Singapore;"},{"name":"Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milly M.","family":"Choy","sequence":"additional","affiliation":[{"name":"Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Nature","first-page":"504","article-title":"The global distribution and burden of dengue","volume":"496:","year":"2013"},{"key":"b2","series-title":"BMC Microbiol","first-page":"9","article-title":"Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes","volume":"7:","year":"2007"},{"key":"b3","series-title":"PLoS Pathog","first-page":"e1006535","article-title":"Dengue subgenomic flaviviral RNA disrupts immunity in mosquito salivary glands to increase virus transmission","volume":"13:","year":"2017"},{"key":"b4","series-title":"Cell Rep","first-page":"107617","article-title":"A non-structural 1 protein G53D substitution attenuates a clinically tested live dengue vaccine","volume":"31:","author":"Choy","year":"2020"},{"key":"b5","series-title":"Am J Trop Med Hyg","first-page":"864","article-title":"Infection, dissemination, 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