{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:19:38Z","timestamp":1788311978979,"version":"build-2803163510"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,9]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Here we report the first detection and confirmation of Powassan virus (POWV) (family: Flaviridae) in <jats:italic>Ixodes scapularis<\/jats:italic> ticks collected from Appalachian Virginia. <jats:italic>Ixodes scapularis<\/jats:italic> ticks were collected from vegetation across field sites in eight counties of western Virginia from June 2019 to April 2021. From these collections, one nymph and one adult male <jats:italic>I. scapularis<\/jats:italic> were determined to be positive for POWV using real-time RT-PCR and Sanger sequencing. Both positive ticks were collected from Floyd county, VA, at residential sites; the nymph in June 2020 and the adult male in April 2021. The presence of POWV in Virginia in its natural tick vector is crucial knowledge in beginning to understand the movement and transmission of this pathogen into new geographical areas and the risk it poses to medical and veterinary health.<\/jats:p>","DOI":"10.4269\/ajtmh.21-0825","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T07:53:08Z","timestamp":1639986788000},"page":"905-908","source":"Crossref","is-referenced-by-count":6,"title":["First Evidence of Powassan Virus (Flaviviridae) in Ixodes scapularis in Appalachian Virginia, USA"],"prefix":"10.4269","volume":"106","author":[{"given":"Alexandra N.","family":"Cumbie","sequence":"first","affiliation":[{"name":"Virginia Polytechnic Institute & State University, Department of Entomology, College of Agriculture and Life Sciences, Blacksburg, Virginia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda M.","family":"Whitlow","sequence":"additional","affiliation":[{"name":"Virginia Polytechnic Institute & State University, Department of Entomology, College of Agriculture and Life Sciences, Blacksburg, Virginia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Eastwood","sequence":"additional","affiliation":[{"name":"Virginia Polytechnic Institute & State University, Department of Entomology, College of Agriculture and Life Sciences, Blacksburg, Virginia;"},{"name":"Virginia Tech Center for Emerging, Zoonotic, and Arthropod-Borne Pathogens (CeZAP), Blacksburg, Virginia;"},{"name":"Virginia Tech Global Change Center, Blacksburg, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Can Med Assoc J","first-page":"708","article-title":"Powassan virus: isolation of virus from a fatal case of encephalitis","volume":"80:","year":"1959"},{"key":"b2","series-title":"Annu Rev 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ticks","volume":"14:","year":"2020"},{"key":"b18","series-title":"Am J Trop Med Hyg","first-page":"971","article-title":"Stable prevalence of Powassan virus in Ixodes scapularis in a northern Wisconsin focus","volume":"79:","year":"2008"},{"key":"b19","series-title":"Am J Trop Med Hyg","first-page":"754","article-title":"Prevalence and genetic characterization of Powassan virus strains infecting Ixodes scapularis in Connecticut","volume":"87:","year":"2012"},{"key":"b20","series-title":"Parasit Vectors","first-page":"1","article-title":"Isolation of deer tick virus (Powassan virus, lineage II) from Ixodes scapularis and detection of antibody in vertebrate hosts sampled in the Hudson Valley, New York State","volume":"6:","year":"2013"}],"container-title":["The American Journal of Tropical Medicine and 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