{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:55Z","timestamp":1787031175068,"version":"build-2736575974"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,4]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>\n                    Asymptomatic hepatitis C virus (HCV) infection without treatment is associated with chronic liver diseases including hepatocellular carcinoma. A major obstacle to hepatitis C diagnosis leading to antiviral treatment in some developing countries is the complicated HCV testing required before treatment. To simplify an HCV test-to-treat strategy, which could lead to timely diagnosis and treatment at the point-of-care, we evaluated the performance of four anti-HCV rapid diagnostic tests (RDTs) (Abon, Blue Cross, Healgen, and SD Bioline). They yielded comparable sensitivity (80\u201383%), specificity (99\u2013100%), and accuracy (90\u201391.5%). When we field-tested Abon in 4,769 residents of an HCV-endemic province in Thailand, 306 seropositive individuals (6.4%) were identified. In comparison, laboratory test using an automated commercial chemiluminescent microparticle immunoassay (ARCHITECT anti-HCV assay, Abbott Diagnostics, Chicago, IL) identified slightly more seropositives (327\/4,769 or 6.9%). Field implementation suggests that Abon was sensitive (88.7%), specific (99.6%), and accurate (98.9%). Furthermore, 82% (250\/306) of Abon-positive samples had detectable HCV RNA as determined by nucleic acid test (cobas\n                    <jats:sup>\u00ae<\/jats:sup>\n                    4800 HCV assay; Roche Diagnostics GmbH, Mannheim, Germany). The same 250 samples out of 327 reactive in Abbott immunoassay also had detectable HCV RNA (mean RNA level: log 6.28 IU\/mL, range: log 3.06\u2013 7.78 IU\/mL). The use of RDT followed by qualitative nucleic acid test can cost-effectively identify the majority of HCV seropositive individuals with active infection, which will obviate the need for expensive viral load quantification tests when simplifying HCV diagnosis for the test-to-treat program at the point-of-care.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.21-1016","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T08:18:55Z","timestamp":1646036335000},"page":"1534-1538","source":"Crossref","is-referenced-by-count":8,"title":["Prescreening with a Rapid Diagnostic Test Followed by a Confirmatory Qualitative Nucleic Acid Test Can Simplify Hepatitis C Diagnosis"],"prefix":"10.4269","volume":"106","author":[{"given":"Rujipat","family":"Wasitthankasem","sequence":"first","affiliation":[{"name":"1National Biobank of Thailand, National Science and Technology Development Agency, Pathum Thani, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawarat","family":"Posuwan","sequence":"additional","affiliation":[{"name":"2Chulabhorn International College of Medicine, Thammasat University, Rangsit Campus, Patum Thani, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Napaporn","family":"Pimsing","sequence":"additional","affiliation":[{"name":"3Phetchabun Provincial Public Health Office, Phetchabun, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wijittra","family":"Phaengkha","sequence":"additional","affiliation":[{"name":"4Nam Nao Hospital, Nam Nao District, Phetchabun, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saranya","family":"Ngamnimit","sequence":"additional","affiliation":[{"name":"3Phetchabun Provincial Public Health Office, Phetchabun, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeyaporn","family":"Vichaiwattana","sequence":"additional","affiliation":[{"name":"5Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilada","family":"Thongpan","sequence":"additional","affiliation":[{"name":"5Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sissades","family":"Tongsima","sequence":"additional","affiliation":[{"name":"1National Biobank of Thailand, National Science and Technology Development Agency, Pathum Thani, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sompong","family":"Vongpunsawad","sequence":"additional","affiliation":[{"name":"5Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Poovorawan","sequence":"additional","affiliation":[{"name":"5Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/5\/article-p1534.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/106\/5\/article-p1534.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T07:39:30Z","timestamp":1651649970000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/5\/article-p1534.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,4]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.21-1016","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,4]]}}}