{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T10:03:53Z","timestamp":1701165833316},"reference-count":53,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>8-Aminoquinoline antimalarial drugs (primaquine, tafenoquine) are required for complete cure of <jats:italic>Plasmodium vivax<\/jats:italic> malaria, but they are contraindicated in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. In the absence of spectrophotometry, which is a gold standard for measuring G6PD activity, <jats:italic>G6PD<\/jats:italic> genotyping is one of the alternatives to establish a database and distribution map of G6PD enzyme deficiency in Mauritania, which has become a new epicenter of <jats:italic>P. vivax<\/jats:italic> malaria in West Africa. The aim of our study was to assess the performance of multiplex allele-specific polymerase chain reaction (PCR) (African-type Diaplex C\u2122 <jats:italic>G6PD<\/jats:italic> kit) against PCR\u2013restriction fragment length polymorphism and sequencing. Of 146 mutations associated with <jats:italic>G6PD<\/jats:italic> A<jats:sup>\u2212<\/jats:sup> genotypes in 177 blood samples from Mauritanian patients, all but two samples were identified correctly using multiplex allele-specific PCR (100% sensitivity and 99% specificity; \u201calmost perfect agreement\u201d between allele-specific PCR and PCR-restriction fragment length polymorphism\/sequencing, with a kappa coefficient of 0.977). Despite a suboptimal PCR protocol for dried blood spots and the inability of the commercial assay to predict unequivocally the G6PD enzyme level in heterozygous females, the African-type Diaplex C\u2122 <jats:italic>G6PD<\/jats:italic> genotyping kit seemed to be a valuable screening tool for male subjects and for research purposes in resource-limited countries where spectrophotometer and DNA sequencing are not available.<\/jats:p>","DOI":"10.4269\/ajtmh.21-1081","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T13:08:26Z","timestamp":1671455306000},"page":"449-455","source":"Crossref","is-referenced-by-count":1,"title":["Performance of a Commercial Multiplex Allele-Specific Polymerase Chain Reaction Kit to Genotype African-Type Glucose-6-Phosphate Dehydrogenase Deficiency"],"prefix":"10.4269","volume":"108","author":[{"given":"Oum Kelthoum Mamadou","family":"Djigo","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche \u201cG\u00e9nomes et Milieux\u201d (Jeune Equipe Associ\u00e9e \u00e0 l\u2019Institut de Recherche pour le D\u00e9veloppement), Facult\u00e9 des Sciences et Techniques, Universit\u00e9 de Nouakchott, Nouakchott, Mauritania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Gomez","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-H\u00f4pitaux de Marseille, Service de Sant\u00e9 des Arm\u00e9es, Vecteurs\u2013Infections Tropicales et M\u00e9diterran\u00e9ennes, Marseille, France;"},{"name":"Institut Hospitalo-Universitaire\u2013M\u00e9diterran\u00e9e Infection, Marseille, France;"},{"name":"Unit\u00e9 de Parasitologie Entomologie, D\u00e9partement de Microbiologie et Maladies Infectieuses, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Salem","family":"Ould Ahmedou Salem","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche \u201cG\u00e9nomes et Milieux\u201d (Jeune Equipe Associ\u00e9e \u00e0 l\u2019Institut de Recherche pour le D\u00e9veloppement), Facult\u00e9 des Sciences et Techniques, Universit\u00e9 de Nouakchott, Nouakchott, Mauritania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Basco","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-H\u00f4pitaux de Marseille, Service de Sant\u00e9 des Arm\u00e9es, Vecteurs\u2013Infections Tropicales et M\u00e9diterran\u00e9ennes, Marseille, France;"},{"name":"Institut Hospitalo-Universitaire\u2013M\u00e9diterran\u00e9e Infection, Marseille, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Ould Mohamed Salem Boukhary","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche 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