{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:53:51Z","timestamp":1780505631996,"version":"3.54.1"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,17]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Standard molecular detection of many pathogens, in particular RNA viruses, requires appropriate handling in the field for preserving the quality of the sample until processing. This could be challenging in remote tropical areas. Dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) are RNA viruses, prominent among the causes of fever in the tropics. We aimed to test the stability of arboviral RNA in contrived dried blood spots prepared on Whatman 903 Protein saver cards as a means of sample collection and storage. We were able to detect DENV, CHIKV, and ZIKV by real-time RT-PCR up to 180 days after card inoculation with stable Ct values across the study period. Our study supports dried blood spots (DBS) on protein saver cards as a platform for stable detection of arboviral RNA of sufficient quality to be used in diagnostic RT-PCR assays and next generation sequencing.<\/jats:p>","DOI":"10.4269\/ajtmh.21-1087","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T13:13:09Z","timestamp":1657026789000},"page":"296-299","source":"Crossref","is-referenced-by-count":6,"title":["Sensitive and Stable Molecular Detection of Dengue, Chikungunya, and Zika Viruses from Dried Blood Spots"],"prefix":"10.4269","volume":"107","author":[{"given":"Jaime A.","family":"Cardona-Ospina","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biomedicina, Facultad de Medicina, Instituci\u00f3n Universitaria Visi\u00f3n de las Am\u00e9ricas, Pereira, Risaralda, Colombia;"},{"name":"Emerging Infectious Diseases and Tropical Medicine Research Group, Sci-help, Pereira, Risaralda, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Stittleburg","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Millan-Benavidez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biomedicina, Facultad de Medicina, Instituci\u00f3n Universitaria Visi\u00f3n de las Am\u00e9ricas, Pereira, Risaralda, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Restrepo-Chica","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biomedicina, Facultad de Medicina, Instituci\u00f3n Universitaria Visi\u00f3n de las Am\u00e9ricas, Pereira, Risaralda, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Autum","family":"Key","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Marcela","family":"Rojas-Gallardo","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biomedicina, Facultad de Medicina, Instituci\u00f3n Universitaria Visi\u00f3n de las Am\u00e9ricas, Pereira, Risaralda, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Piantadosi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, Georgia;"},{"name":"Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew H.","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, Georgia;"},{"name":"Department of Global Health, Rollins School of Public Health, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse J.","family":"Waggoner","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, Georgia;"},{"name":"Department of Global Health, Rollins School of Public Health, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Trop Med Infect Dis","article-title":"Fatal dengue, chikungunya and leptospirosis: the importance of assessing co-infections in febrile patients in tropical areas","volume":"3","year":"2018"},{"key":"b2","series-title":"Prev Vet Med","article-title":"A systematic review of FTA cards\u00ae as a tool for 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