{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:01:35Z","timestamp":1787313695821,"version":"build-2736575974"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,4]]},"abstract":"ABSTRACT.<\/jats:title>\n \n Molecular methods are necessary to detect low-density malaria infections. The purpose of this study was to assess the diagnostic performance of six malachite-green loop-mediated amplification method (MG-LAMP) assays (MG-LAMP-\n Pf<\/jats:italic>\n , MG-LAMP-\n Pv<\/jats:italic>\n , MG-LAMP-\n Po<\/jats:italic>\n , MG-LAMP-\n Pm<\/jats:italic>\n , MG-LAMP-\n Pk<\/jats:italic>\n , and MG-LAMP-\n P<\/jats:italic>\n spp) for the species-specific detection of each human\n Plasmodium<\/jats:italic>\n , including\n P. knowlesi<\/jats:italic>\n , and the\n Plasmodium<\/jats:italic>\n genus compared with the nested-multiplex malaria polymerase chain reaction (NM-PCR), using 161 malaria-positive and 274 malaria-negative samples. MG-LAMP-\n P<\/jats:italic>\n spp assay detected the five human\n Plasmodium<\/jats:italic>\n species and each species-specific MG-LAMP assay detected only its corresponding species. Sensitivity, specificity, and predictive values of MG-LAMP assays, compared with NM-PCR, were >\u00a090%, except in the case of the MG-LAMP-\n Pm<\/jats:italic>\n assay, which dropped to 47%. Limit of detection for MG-LAMP-\n P<\/jats:italic>\n spp assay ranged from 0.1 parasites\/\u00b5L for\n P. falciparum<\/jats:italic>\n to 16.9 parasites\/\u00b5L for\n P. malariae<\/jats:italic>\n samples, and it was similar for the rest of MG-LAMP assays except for the MG-LAMP-\n Pm<\/jats:italic>\n assay. Turnaround time was estimated to be 2 hours and 35 minutes for one MG-LAMP assay and 4 hours and 15 minutes if all species-specific MG-LAMP is set up, whereas for the NM-PCR, turnaround time was \u223c6 hours and 15 minutes. Costs per determination ranged from 1 to 6 euros for MG-LAMP assays and 5 euros for NM-PCR. Therefore, MG-LAMP assays appear to have good concordance compared with the reference method, except for the MG-LAMP-\n Pm<\/jats:italic>\n assay. They can detect low parasitemia and identify malaria species, with lower costs and shorter time to obtain results, and they are suitable tools to be used in endemic and non-endemic countries for malaria detection.\n <\/jats:p>","DOI":"10.4269\/ajtmh.21-1151","type":"journal-article","created":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T08:32:49Z","timestamp":1647246769000},"page":"1414-1420","source":"Crossref","is-referenced-by-count":5,"title":["Usefulness of Malachite-Green LAMP for Diagnosis of Plasmodium and Five Human Malaria Species in a Nonendemic Setting"],"prefix":"10.4269","volume":"106","author":[{"given":"Alexandra","family":"Mart\u00edn-Ram\u00edrez","sequence":"first","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, National Microbiology Centre, Instituto de Salud Carlos III, Madrid, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Lanza-Su\u00e1rez","sequence":"additional","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, National Microbiology Centre, Instituto de Salud Carlos III, Madrid, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlota","family":"Mu\u00f1oz-Garc\u00eda","sequence":"additional","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, National Microbiology Centre, Instituto de Salud Carlos III, Madrid, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamilah R.","family":"Hisam","sequence":"additional","affiliation":[{"name":"2Parasitology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institute of Health, Ministry of Health Malaysia, Setia Alam, Selangor, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Perez-Ayala","sequence":"additional","affiliation":[{"name":"3Department of Clinical Microbiology, Hospital Universitario 12 de Octubre, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Rubio","sequence":"additional","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, National Microbiology Centre, Instituto de Salud Carlos III, Madrid, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/5\/article-p1414.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/106\/5\/article-p1414.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T07:40:05Z","timestamp":1651650005000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/5\/article-p1414.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,4]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.21-1151","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,4]]}}}