{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T18:31:29Z","timestamp":1784399489489,"version":"3.55.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,13]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Despite recent success in reducing the regional incidence of <jats:italic>Plasmodium falciparum<\/jats:italic> malaria, cases of zoonotic malaria are on the rise in Southeast Asia. The Cambodian National Malaria Surveillance Program has previously relied on rapid diagnostic tests and blood smear microscopy with confirmatory polymerase chain reaction (PCR) testing in a subset of cases to further distinguish <jats:italic>P. falciparum, P. malariae, P. ovale,<\/jats:italic> and <jats:italic>P. vivax<\/jats:italic> species. Here, metagenomic next-generation sequencing identified <jats:italic>P. knowlesi<\/jats:italic> mono-infection in six Cambodian patients initially diagnosed with <jats:italic>P. malariae<\/jats:italic> by blood smear microscopy in February\u2013May 2020. These findings of recent human infections with <jats:italic>P. knowlesi<\/jats:italic> in Cambodia led to the incorporation of <jats:italic>P. knowlesi<\/jats:italic>\u2013specific PCR diagnostics to national malaria surveillance efforts.<\/jats:p>","DOI":"10.4269\/ajtmh.22-0039","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T17:42:16Z","timestamp":1657734136000},"page":"151-153","source":"Crossref","is-referenced-by-count":11,"title":["Case Report: Cambodian National Malaria Surveillance Program Detection of Plasmodium knowlesi"],"prefix":"10.4269","volume":"107","author":[{"given":"Christina","family":"Yek","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine, National Institutes of Health Clinical Center, Bethesda, Maryland;"},{"name":"Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, 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