{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T06:35:37Z","timestamp":1783406137532,"version":"3.54.6"},"reference-count":11,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Antigen detected in urine for the diagnosis of opisthorchiasis has a low daily variation; however, the longer term variability in antigen concentrations is unknown. In this study, we prospectively monitored <jats:italic>Opisthorchis viverrini<\/jats:italic> antigen concentrations for 30 consecutive days and at subsequent monthly intervals in a cohort of opisthorchiasis-positive individuals. On the basis of the monoclonal antibody\u2013based ELISA, the profiles of antigen-positive rate and antigen concentration exhibited no significant change over 30 days with a mean proportion positive of 87.1% (range 73.7%\u2013100%), and the average antigen concentration was 29.7 \u00b1 2.2 ng\/mL (mean \u00b1 SE). The urine antigen concentration at baseline was similar to the subsequent measurements at 2, 4, 6, and 10 months in the follow-up study (<jats:italic>P<\/jats:italic> &gt; 0.05). The consistency and low daily and long-term fluctuation of <jats:italic>O. viverrini<\/jats:italic> antigen in urine demonstrates the reliability of urine assay for diagnosis of opisthorchiasis.<\/jats:p>","DOI":"10.4269\/ajtmh.22-0478","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T14:15:19Z","timestamp":1672064119000},"page":"356-358","source":"Crossref","is-referenced-by-count":5,"title":["Long-term Persistence of Opisthorchis viverrini Antigen in Urine: A Prospective Study in Northeast Thailand"],"prefix":"10.4269","volume":"108","author":[{"given":"Chanika","family":"Worasith","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand;"},{"name":"Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phattharaphon","family":"Wongphutorn","sequence":"additional","affiliation":[{"name":"Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kulthida Y.","family":"Kopolrat","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chutima","family":"Homwong","sequence":"additional","affiliation":[{"name":"Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchalee","family":"Techasen","sequence":"additional","affiliation":[{"name":"Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raynoo","family":"Thanan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chompunoot","family":"Wangboon","sequence":"additional","affiliation":[{"name":"School of Preclinical Science, Institute of Science, Suranaree University of Technology, Nakhon Rachasima, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chatanun","family":"Eamudomkarn","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand;"},{"name":"Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiraporn","family":"Sithithaworn","sequence":"additional","affiliation":[{"name":"Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Crellen","sequence":"additional","affiliation":[{"name":"School of Biodiversity, One Health and Veterinary Medicine, Glasgow, United Kingdom;"},{"name":"Big Data Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paiboon","family":"Sithithaworn","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand;"},{"name":"Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Parasitol Int","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.parint.2011.08.014","article-title":"The current status of opisthorchiasis and clonorchiasis in the Mekong Basin","volume":"61","year":"2012"},{"key":"b2","series-title":"Acta Trop","doi-asserted-by":"publisher","first-page":"106133","DOI":"10.1016\/j.actatropica.2021.106133","article-title":"Current status of human liver fluke infections in the Greater Mekong Subregion","volume":"224","year":"2021"},{"key":"b3","series-title":"Trends Parasitol","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/j.pt.2020.12.007","article-title":"Towards evidence-based control of Opisthorchis viverrini.","volume":"37","year":"2021"},{"key":"b4","series-title":"Front Med (Lausanne)","first-page":"117","article-title":"Current perspectives on opisthorchiasis control and cholangiocarcinoma 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