{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T14:48:53Z","timestamp":1777906133958,"version":"3.51.4"},"reference-count":55,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>The endosymbiotic <jats:italic>Leishmania<\/jats:italic> RNA virus 1 (LRV1) has been associated with severity and clinical manifestations of American tegumentary leishmaniasis caused by species of the <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Viannia<\/jats:italic>) subgenus. Between and within <jats:italic>Leishmania<\/jats:italic> species, and among endemic countries, the prevalence of LRV is highly variable. The LRV virus has not been detected in <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>panamensis<\/jats:italic>, the second-most prevalent species in Central America and Colombia. However, no systematic screening of LRV has been conducted in <jats:italic>L. <\/jats:italic>(<jats:italic>V<\/jats:italic>.) <jats:italic>panamensis<\/jats:italic>, and thus it is still controversial whether this virus is truly absent from the species. We sought to determine the prevalence of LRV1 in <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>panamensis<\/jats:italic> clinical strains isolated from patients with cutaneous leishmaniasis (CL), from different geographic areas of Colombia. We analyzed 219 clinical strains; 78% were <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>panamensis<\/jats:italic>, 18% were <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic>, and 4% were <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>guyanensis<\/jats:italic>. Screening for LRV1 was performed by quantitative reverse transcription\u2013polymerase chain reaction. The LRV1 was detected in 18% (7 of 40) of <jats:italic>L.<\/jats:italic> (<jats:italic>V<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic> strains, and was not detected in any of the <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>guyanensis<\/jats:italic> or <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>panamensis<\/jats:italic> strains. The LRV1-positive <jats:italic>L.<\/jats:italic> (<jats:italic>V<\/jats:italic>). <jats:italic>braziliensis<\/jats:italic> strains came from the Amazon Basin. Of the seven LRV1-positive strains, two were isolated from patients with mucocutaneous leishmaniasis, and the remaining from patients with CL. Our results confirm the absence of LRV1 in <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>panamensis<\/jats:italic> in Colombia.<\/jats:p>","DOI":"10.4269\/ajtmh.22-0551","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T14:21:57Z","timestamp":1675088517000},"page":"555-560","source":"Crossref","is-referenced-by-count":8,"title":["Leishmania RNA Virus Is Not Detected in All Species of the Leishmania Viannia Subgenus: The Case of L. (V.) panamensis in Colombia"],"prefix":"10.4269","volume":"108","author":[{"given":"Mariana","family":"Rosales-Chilama","sequence":"first","affiliation":[{"name":"Centro Internacional de Entrenamiento e Investigaciones M\u00e9dicas-CIDEIM, Cali, Colombia;"},{"name":"Universidad Icesi, Cali, Colombia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Y. Oviedo","sequence":"additional","affiliation":[{"name":"Centro Internacional de Entrenamiento e Investigaciones M\u00e9dicas-CIDEIM, Cali, Colombia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yury","family":"K. Quintero","sequence":"additional","affiliation":[{"name":"Centro Internacional de Entrenamiento e Investigaciones M\u00e9dicas-CIDEIM, Cali, Colombia;"},{"name":"Universidad Industrial de Santander, Bucaramanga, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"L. Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro Internacional de Entrenamiento e Investigaciones M\u00e9dicas-CIDEIM, Cali, Colombia;"},{"name":"Universidad Icesi, Cali, Colombia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Adelaida","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Centro Internacional de Entrenamiento e Investigaciones M\u00e9dicas-CIDEIM, Cali, Colombia;"},{"name":"Universidad Icesi, Cali, Colombia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Nature","first-page":"588","article-title":"Virus-like particles in Leishmania parasites","volume":"249","author":"Molyneux","year":"1974"},{"key":"b2","series-title":"Proc Natl Acad Sci USA","first-page":"9572","article-title":"LR1: a candidate RNA virus of Leishmania","volume":"85","author":"Tarr","year":"1988"},{"key":"b3","series-title":"Proc Natl Acad Sci USA","first-page":"8596","article-title":"Molecular organization of Leishmania RNA virus 1","volume":"89","author":"Stuart","year":"1992"},{"key":"b4","series-title":"Mol Biochem Parasitol","first-page":"101","article-title":"Distribution and sequence divergence of LRV1 viruses among different Leishmania species","volume":"54","author":"Guilbride","year":"1992"},{"key":"b5","series-title":"Nucleic Acids Res","first-page":"2300","article-title":"Phylogenetic analysis of Leishmania RNA virus and Leishmania suggests ancient virus-parasite association","volume":"23","author":"Widmer","year":"1995"},{"key":"b6","series-title":"Am J Trop Med Hyg","first-page":"425","article-title":"Leishmania RNA viruses in Leishmania of the Viannia subgenus","volume":"54","author":"Salinas","year":"1996"},{"key":"b7","series-title":"Am J Trop Med Hyg","first-page":"192","article-title":"Short report: detection of leishmaniavirus in human biopsy samples of leishmaniasis from Peru","volume":"58","author":"Saiz","year":"1998"},{"key":"b8","series-title":"Mem Inst Oswaldo Cruz","first-page":"665","article-title":"Severity of tegumentary leishmaniasis is not exclusively associated with Leishmania RNA virus 1 infection in Brazil","volume":"108","author":"de Oliveira Ramos Pereira","year":"2013"},{"key":"b9","series-title":"PLoS Negl Trop Dis","first-page":"e0004079","article-title":"Further evidence of an association between the presence of Leishmania RNA virus 1 and the mucosal manifestations in tegumentary leishmaniasis patients","volume":"9","author":"Cantanh\u00eade","year":"2015"},{"key":"b10","series-title":"Am J Trop Med Hyg","first-page":"102","article-title":"Prevalence and distribution of Leishmania RNA virus 1 in Leishmania parasites from French Guiana","volume":"94","author":"Ginouv\u00e8s","year":"2016"},{"key":"b11","series-title":"Mol Biochem Parasitol","first-page":"50","article-title":"Low frequency of LRV1 in Leishmania braziliensis strains isolated from typical and atypical lesions in the State of Minas Gerais, Brazil","volume":"210","author":"Macedo","year":"2016"},{"key":"b12","series-title":"PLoS Negl Trop Dis","first-page":"e0010162","article-title":"Leishmania RNA virus-1 is similarly detected among metastatic and non-metastatic phenotypes in a prospective cohort of American tegumentary leishmaniasis","volume":"16","author":"Valencia","year":"2022"},{"key":"b13","series-title":"J Infect Dis","first-page":"112","article-title":"Association of the endobiont double-stranded RNA virus LRV1 with treatment failure for human leishmaniasis caused by Leishmania braziliensis in Peru and Bolivia","volume":"213","author":"Adaui","year":"2016"},{"key":"b14","series-title":"PLoS One","first-page":"e0198727","article-title":"New insights into the genetic diversity of Leishmania RNA virus 1 and its species-specific relationship with Leishmania parasites","volume":"13","author":"Cantanh\u00eade","year":"2018"},{"key":"b15","series-title":"Rev Soc Bras Med Trop","first-page":"e20180323","article-title":"First report of treatment failure in a patient with cutaneous leishmaniasis infected by Leishmania (Viannia) naiffi carrying Leishmania RNA virus: a fortuitous combination?","volume":"52","author":"Vieira-Gon\u00e7alves","year":"2019"},{"key":"b16","series-title":"PLoS Negl Trop Dis","first-page":"e2836","article-title":"Leishmania aethiopica field isolates bearing an endosymbiontic dsRNA virus induce pro-inflammatory cytokine response","volume":"8","author":"Zangger","year":"2014"},{"key":"b17","series-title":"Am J Trop Med Hyg","first-page":"309","article-title":"Short report: quantification of Leishmaniavirus RNA in clinical samples and its possible role in pathogenesis","volume":"69","author":"Ogg","year":"2003"},{"key":"b18","series-title":"Braz J Otorhinolaryngol","first-page":"533","article-title":"Correlation between presence of Leishmania RNA virus 1 and clinical characteristics of nasal mucosal leishmaniosis","volume":"81","author":"Ito","year":"2015"},{"key":"b19","series-title":"J Infect Dis","first-page":"105","article-title":"Presence of Leishmania RNA virus 1 in Leishmania guyanensis increases the risk of first-line treatment failure and symptomatic relapse","volume":"213","author":"Bourreau","year":"2016"},{"key":"b20","series-title":"Virology","first-page":"84","article-title":"The complete sequence of Leishmania RNA virus LRV2-1, a virus of an Old World parasite strain","volume":"212","author":"Scheffter","year":"1995"},{"key":"b21","series-title":"Arch Virol","first-page":"3385","article-title":"Detection and molecular identification of Leishmania RNA virus (LRV) in Iranian Leishmania species","volume":"161","author":"Hajjaran","year":"2016"},{"key":"b22","series-title":"Trans R Soc Trop Med Hyg","first-page":"410","article-title":"Detection of Leishmania RNA virus 2 in Leishmania species from Turkey","volume":"113","author":"Nal\u00e7ac\u0131","year":"2019"},{"key":"b23","series-title":"New Microbiol","first-page":"65","article-title":"First report and in silico analysis of Leishmania virus (LRV2) identified in an autochthonous Leishmania major isolate in Turkey","volume":"42","author":"Kurt","year":"2019"},{"key":"b24","series-title":"PLoS Negl Trop Dis","first-page":"e0005764","article-title":"Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana","volume":"11","author":"Tirera","year":"2017"},{"key":"b25","series-title":"Viruses","first-page":"2305","article-title":"Analyses of Leishmania-LRV co-phylogenetic patterns and evolutionary variability of viral proteins","volume":"13","author":"Kostygov","year":"2021"},{"key":"b26","series-title":"BMC Infect Dis","first-page":"938","article-title":"Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis","volume":"20","author":"Correa-C\u00e1rdenas","year":"2020"},{"key":"b27","series-title":"Am J Trop Med Hyg","first-page":"840","article-title":"Case report: severe cutaneous leishmaniasis in a human immunodeficiency virus patient coinfected with Leishmania braziliensis and its endosymbiotic virus","volume":"94","author":"Parmentier","year":"2016"},{"key":"b28","series-title":"Nat Commun","first-page":"5273","article-title":"Leishmania RNA virus exacerbates leishmaniasis by subverting innate immunity via TLR3-mediated NLRP3 inflammasome inhibition","volume":"10","author":"de Carvalho","year":"2019"},{"key":"b29","series-title":"PLoS Negl Trop Dis","first-page":"e2006","article-title":"Detection of Leishmania RNA virus in Leishmania parasites","volume":"7","author":"Zangger","year":"2013"},{"key":"b30","series-title":"Science","first-page":"775","article-title":"Leishmania RNA virus controls the severity of mucocutaneous leishmaniasis","volume":"331","author":"Ives","year":"2011"},{"key":"b31","series-title":"Nat Microbiol","first-page":"714","article-title":"Exploitation of the Leishmania exosomal pathway by Leishmania RNA virus 1","volume":"4","author":"Atayde","year":"2019"},{"key":"b32","series-title":"BMC Res Notes","first-page":"126","article-title":"Relationship of Leishmania RNA virus (LRV) and treatment failure in clinical isolates of Leishmania major","volume":"13","author":"Abtahi","year":"2020"},{"key":"b33","series-title":"Clin Microbiol Infect","first-page":"286.e1","article-title":"Leishmaniavirus genetic diversity is not related to leishmaniasis treatment failure","volume":"27","author":"Ginouv\u00e8s","year":"2021"},{"key":"b34","series-title":"Am J Trop Med Hyg","first-page":"280","article-title":"Leishmania RNA virus 1 (LRV-1) in Leishmania (Viannia) braziliensis isolates from Peru: a description of demographic and clinical correlates","volume":"102","author":"Kariyawasam","year":"2020"},{"key":"b35","series-title":"Am J Trop Med Hyg","first-page":"233","article-title":"Detection of Leishmania RNA virus in clinical samples from cutaneous leishmaniasis patients varies according to the type of sample","volume":"104","author":"Parra-Mu\u00f1oz","year":"2021"},{"key":"b36","series-title":"Biomedica","first-page":"278","article-title":"Geographical distribution of Leishmania species in Colombia, 1985\u20132017","volume":"39","author":"Salgado-Almario","year":"2019"},{"key":"b37","series-title":"Am J Trop Med Hyg","first-page":"206","article-title":"Distribution and etiology of leishmaniasis in Colombia","volume":"42","author":"Corredor","year":"1990"},{"key":"b38","series-title":"Am J Trop Med Hyg","first-page":"86","article-title":"Epidemiologic, genetic, and clinical associations among phenotypically distinct populations of Leishmania (Viannia) in Colombia","volume":"59","author":"Saravia","year":"1998"},{"key":"b39","series-title":"Am J Trop Med Hyg","first-page":"738","article-title":"Heterogeneity, geographic distribution, and pathogenicity of serodemes of Leishmania Viannia in Colombia","volume":"66","author":"Saravia","year":"2002"},{"key":"b40","series-title":"Am J Trop Med Hyg","first-page":"49","article-title":"Mucosal leishmaniasis due to Leishmania (Viannia) panamensis in Colombia: clinical characteristics","volume":"59","author":"Osorio","year":"1998"},{"key":"b41","series-title":"PLoS One","first-page":"e0214124","article-title":"Evaluating the spatial distribution of Leishmania parasites in Colombia from clinical samples and human isolates (1999 to 2016)","volume":"14","author":"Ovalle-Bracho","year":"2019"},{"key":"b42","series-title":"PLoS Negl Trop Dis","first-page":"e0005876","article-title":"Spatial distribution, Leishmania species and clinical traits of cutaneous leishmaniasis cases in the Colombian army","volume":"11","author":"Patino","year":"2017"},{"key":"b43","series-title":"Mem Inst Oswaldo Cruz","first-page":"37","article-title":"Monoclonal antibodies for the identification of New World Leishmania species","volume":"91","author":"Grimaldi","year":"1996"},{"key":"b44","series-title":"Trans R Soc Trop Med Hyg","first-page":"35","article-title":"The isolation and isoenzyme characterization of Leishmania braziliensis subsp. from patients with cutaneous leishmaniasis acquired in Belize","volume":"78","author":"Evans","year":"1984"},{"key":"b45","series-title":"Am J Trop Med Hyg","first-page":"296","article-title":"A general classification of New World Leishmania using numerical zymotaxonomy","volume":"50","author":"Cupolillo","year":"1994"},{"key":"b46","series-title":"Am J Trop Med Hyg","first-page":"276","article-title":"Etiologic agent of an epidemic of cutaneous leishmaniasis in Tolima, Colombia","volume":"78","author":"Rodr\u00edguez-Barraquer","year":"2008"},{"key":"b47","series-title":"Acta Trop","first-page":"355","article-title":"Profiling gene expression of antimony response genes in Leishmania (Viannia) panamensis and infected macrophages and its relationship with drug susceptibility","volume":"176","author":"Barrera","year":"2017"},{"key":"b48","series-title":"Infect Immun","first-page":"e00881-19","article-title":"Immune profile of the nasal mucosa in patients with cutaneous leishmaniasis","volume":"88","author":"G\u00f3mez-Zafra","year":"2020"},{"key":"b49","series-title":"Trop Med Health","first-page":"25","article-title":"Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1","volume":"47","author":"Kariyawasam","year":"2019"},{"key":"b50","series-title":"Med Microbiol Immunol (Berl)","first-page":"57","article-title":"Recent advances in the taxonomy of the New World leishmanial parasites","volume":"190","author":"Cupolillo","year":"2001"},{"key":"b51","series-title":"Diagn Microbiol Infect Dis","first-page":"432","article-title":"An enhanced method for the identification of Leishmania spp. using real-time polymerase chain reaction and sequence analysis of the 7SL RNA gene region","volume":"66","author":"Stevenson","year":"2010"},{"key":"b52","series-title":"Genes (Basel)","first-page":"830","article-title":"Molecular characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan","volume":"10","author":"Kleschenko","year":"2019"},{"key":"b53","series-title":"Int J Infect Dis","first-page":"6","article-title":"Presence and diversity of Leishmania RNA virus in an old zoonotic cutaneous leishmaniasis focus, northeastern Iran: haplotype and phylogenetic based approach","volume":"101","author":"Saberi","year":"2020"},{"key":"b54","series-title":"Genes (Basel)","first-page":"657","article-title":"The maze pathway of coevolution: a critical review over the Leishmania and its endosymbiotic history","volume":"12","author":"Cantanh\u00eade","year":"2021"},{"key":"b55","series-title":"Front Cell Infect Microbiol","first-page":"687607","article-title":"Early leukocyte responses in ex-vivo models of healing and non-healing human Leishmania (Viannia) panamensis infections","volume":"11","author":"Gomez","year":"2021"}],"container-title":["The American Journal of Tropical Medicine and 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