{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:39:49Z","timestamp":1778693989071,"version":"3.51.4"},"reference-count":36,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>This study highlights the development of two lateral flow recombinase polymerase amplification assays for the diagnosis of human malaria. The lateral flow cassettes contained test lines that captured biotin-, 6-carboxyfluorescein, digoxigenin-, cyanine 5-, and dinitrophenyl-labeled amplicons. The overall process can be completed in 30 minutes. Recombinase polymerase amplification coupled with lateral flow had a detection limit of 1 copy\/\u00b5L for <jats:italic>Plasmodium knowlesi<\/jats:italic>, <jats:italic>Plasmodium vivax<\/jats:italic>, and <jats:italic>Plasmodium falciparum<\/jats:italic>. No cross-reactivity was observed among nonhuman malaria parasites such as <jats:italic>Plasmodium coatneyi<\/jats:italic>,<jats:italic> Plasmodium cynomolgi<\/jats:italic>,<jats:italic> Plasmodium brasilanium<\/jats:italic>, <jats:italic>Plasmodium inui<\/jats:italic>,<jats:italic> Plasmodium fragile<\/jats:italic>,<jats:italic> Toxoplasma gondii<\/jats:italic>, <jats:italic>Sarcocystis<\/jats:italic> spp., <jats:italic>Brugia<\/jats:italic> spp., and 20 healthy donors. It is rapid, highly sensitive, robust, and easy to use. The result can be read without the need for special equipment and thus has the potential to serve as an effective alternative to polymerase chain reaction methods for the diagnosis of malaria.<\/jats:p>","DOI":"10.4269\/ajtmh.22-0657","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T12:30:36Z","timestamp":1678710636000},"page":"882-886","source":"Crossref","is-referenced-by-count":2,"title":["Lateral Flow Recombinase Polymerase Amplification Assays for the Detection of Human Plasmodium Species"],"prefix":"10.4269","volume":"108","author":[{"given":"Meng Yee","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd","family":"Abdul Hamid","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenarun","family":"Jelip","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose Nani","family":"Mudin","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"World Malaria Report 2021","year":"2021"},{"key":"b2","series-title":"PLoS Biol","first-page":"e204","article-title":"DNA detection using recombination 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