{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:13:44Z","timestamp":1790140424069,"version":"4.0.1"},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Much of our understanding of malaria transmission comes from mosquito feeding assays using <jats:italic>Anopheles<\/jats:italic> mosquitoes from colonies that are well adapted to membrane feeding. This raises the question whether results from colony mosquitoes lead to overestimates of outcomes in wild <jats:italic>Anopheles<\/jats:italic> mosquitoes. We successfully established an <jats:italic>Anopheles<\/jats:italic> colony using progeny of wild <jats:italic>Anopheles gambiae<\/jats:italic> s.s. mosquitoes (Busia mosquitoes) and directly compared their susceptibility to infection with <jats:italic>Plasmodium falciparum<\/jats:italic> with the widely used <jats:italic>An. gambiae<\/jats:italic> s.s. mosquitoes (Kisumu mosquitoes) using gametocyte-infected Ugandan donor blood. The proportion of infectious feeds did not differ between Busia (71.8%, 23\/32) and Kisumu (68.8%, 22\/32, <jats:italic>P<\/jats:italic> = 1.00) mosquitoes. When correcting for random effects of donor blood, we observed a 23% higher proportion of infected Busia mosquitoes than infected Kisumu mosquitoes (RR, 1.23; 95% CI, 1.10\u20131.38, <jats:italic>P &lt;<\/jats:italic> 0.001). This study suggests that feeding assays with Kisumu mosquitoes do not overestimate outcomes in wild <jats:italic>An. gambiae<\/jats:italic> s.s. mosquitoes, the mosquito species most relevant to malaria transmission in Uganda.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0203","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T14:16:23Z","timestamp":1703686583000},"page":"209-213","source":"Crossref","is-referenced-by-count":2,"title":["Susceptibility of Anopheles gambiae to Natural Plasmodium falciparum Infection: A Comparison between the Well-Established Anopheles gambiae s.s Line and a Newly Established Ugandan Anopheles gambiae s.s. Line"],"prefix":"10.4269","volume":"110","author":[{"given":"Daniel","family":"Ayo","sequence":"first","affiliation":[{"name":"Infectious Diseases Research Collaboration, Nagongera Hospital, Tororo, Uganda;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Onyige","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Collaboration, Nagongera Hospital, Tororo, Uganda;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Okoth","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Collaboration, Nagongera Hospital, Tororo, Uganda;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Musasizi","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Collaboration, Nagongera Hospital, Tororo, Uganda;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambrose","family":"Oruni","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordache","family":"Ramjith","sequence":"additional","affiliation":[{"name":"Department for Health Evidence, Radboud University Medical Center, Nijmegen, Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Arinaitwe","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Collaboration, Nagongera Hospital, Tororo, Uganda;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Rek","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Collaboration, Nagongera Hospital, Tororo, Uganda;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Drakeley","sequence":"additional","affiliation":[{"name":"Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah G.","family":"Staedke","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Donnelly","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teun","family":"Bousema","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Conrad","sequence":"additional","affiliation":[{"name":"Department of Medicine, San Francisco General Hospital, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Lynn","family":"Blanken","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"World Malaria Report 2022","year":"2022"},{"key":"b2","series-title":"Malar J","article-title":"Reflections on the 2021 World Malaria Report and the future of malaria control","volume":"21","author":"Monroe","year":"2022"},{"key":"b3","series-title":"Parasit Vectors","first-page":"72","article-title":"The dominant Anopheles vectors of human malaria in the Americas: occurrence data, 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Insectary establishment and identification of Plasmodium falciparum oocysts in midguts of Anopheles mosquitoes fed on infected human blood in Tororo, Uganda","volume":"18","author":"Musiime","year":"2019"},{"key":"b18","series-title":"Trans R Soc Trop Med Hyg","first-page":"187","article-title":"The effect of anticoagulants in blood collection tubes on Plasmodium falciparum transmission in direct membrane feeding assays","volume":"116","author":"Graumans","year":"2022"},{"key":"b19","series-title":"Methodology","first-page":"315","article-title":"Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays","volume":"16","author":"Reuling","year":"2017"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/110\/2\/article-p209.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/110\/2\/article-p209.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/110\/2\/article-p209.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T12:51:32Z","timestamp":1744980692000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/110\/2\/article-p209.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,7]]},"references-count":19,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.23-0203","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,7]]}}}