{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:31:09Z","timestamp":1789428669134,"version":"build-2803163510"},"reference-count":36,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","license":[{"start":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T00:00:00Z","timestamp":1709683200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,6]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Dantu erythrocytes, which express a hybrid glycophorin B\/A protein, are protective against severe malaria. Recent studies have shown that Dantu impairs <jats:italic>Plasmodium falciparum<\/jats:italic> invasion by increasing erythrocyte membrane tension, but its effects on pathological host\u2013parasite adhesion interactions such as rosetting, the binding of uninfected erythrocytes to <jats:italic>P. falciparum<\/jats:italic>\u2013infected erythrocytes, have not been investigated previously. The expression of several putative host rosetting receptors\u2014including glycophorin A (GYPA), glycophorin C (GYPC), complement receptor 1 (CR1), and band 3, which complexes with GYPA to form the Wright<jats:sup>b<\/jats:sup> blood group antigen\u2014are altered on Dantu erythrocytes. Here, we compare receptor expression, and rosetting at both 1\u2009hour and 48\u2009hours after mixing with mature trophozoite-stage Kenyan laboratory\u2013adapted <jats:italic>P. falciparum<\/jats:italic> strain 11019 parasites in Dantu and non-Dantu erythrocytes. Dantu erythrocytes showed lower staining for GYPA and CR1, and greater staining for band 3, as observed previously, whereas Wright<jats:sup>b<\/jats:sup> and GYPC staining did not vary significantly. No significant between-genotype differences in rosetting were seen after 1\u2009hour, but the percentage of large rosettes was significantly less in both Dantu heterozygous (mean, 16.4%; standard error of the mean [SEM], 3.2) and homozygous donors (mean, 15.4%; SEM, 1.4) compared with non-Dantu erythrocytes (mean, 32.9%; SEM, 7.1; one-way analysis of variance, <jats:italic>P =<\/jats:italic> 0.025) after 48\u2009hours. We also found positive correlations between erythrocyte mean corpuscular volume (MCV), the percentage of large rosettes (Spearman\u2019s r<jats:sub>s<\/jats:sub> = 0.5970, <jats:italic>P =<\/jats:italic> 0.0043), and mean rosette size (r<jats:sub>s<\/jats:sub> = 0.5206, <jats:italic>P =<\/jats:italic> 0.0155). Impaired rosetting resulting from altered erythrocyte membrane receptor expression and reduced MCV might add to the protective effect of Dantu against severe malaria.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0347","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T13:46:35Z","timestamp":1706708795000},"page":"436-443","source":"Crossref","is-referenced-by-count":2,"title":["Dantu Blood Group Erythrocytes Form Large Plasmodium falciparum Rosettes Less Commonly"],"prefix":"10.4269","volume":"110","author":[{"given":"Molly S. 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Alexandra","family":"Rowe","sequence":"additional","affiliation":[{"name":"Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia N.","family":"Kariuki","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Demography, KEMRI\u2013Wellcome Trust Research Programme, Kilifi, Kenya;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"World Malaria Report 2020: 20 Years of Global Progress and Challenges","year":"2020"},{"key":"b2","series-title":"Science","first-page":"555","article-title":"Falciparum malaria-infected erythrocytes specifically bind to cultured human endothelial cells","volume":"213","author":"Udeinya","year":"1981"},{"key":"b3","series-title":"J Exp Med","first-page":"1835","article-title":"Plasmodium falciparum-infected erythrocytes form spontaneous erythrocyte rosettes","volume":"169","author":"Udomsangpetch","year":"1989"},{"key":"b4","series-title":"Blood","first-page":"812","article-title":"Rosetting of Plasmodium falciparum-infected red blood cells with uninfected red blood cells enhances microvascular obstruction under flow conditions","volume":"78","author":"Kaul","year":"1991"},{"key":"b5","series-title":"J Infect Dis","first-page":"263","article-title":"Perfusion abnormalities in children with cerebral malaria and malarial retinopathy","volume":"199","author":"Beare","year":"2009"},{"key":"b6","series-title":"Curr Top Microbiol Immunol","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/3-540-29088-5_5","article-title":"Metabolic complications of severe malaria","volume":"295","author":"Planche","year":"2005"},{"key":"b7","series-title":"PLoS Pathog","first-page":"e1009122","article-title":"Etiology of lactic acidosis in malaria","volume":"17","author":"Possemiers","year":"2021"},{"key":"b8","series-title":"Nature","first-page":"292","article-title":"P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1","volume":"388","author":"Rowe","year":"1997"},{"key":"b9","series-title":"Expert Rev Mol Med","first-page":"e16","article-title":"Adhesion of Plasmodium falciparum-infected erythrocytes to human cells: molecular mechanisms and therapeutic implications","volume":"11","author":"Rowe","year":"2009"},{"key":"b10","series-title":"Parasitology","first-page":"1","article-title":"Rosetting revisited: a critical look at the evidence for host erythrocyte receptors in Plasmodium falciparum rosetting","volume":"147","author":"McQuaid","year":"2020"},{"key":"b11","series-title":"J Exp Med","first-page":"1311","article-title":"Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions","volume":"176","author":"Carlson","year":"1992"},{"key":"b12","series-title":"J Immunol","first-page":"6341","article-title":"Mapping of the region of complement receptor (CR) 1 required for Plasmodium falciparum rosetting and demonstration of the importance of CR1 in rosetting in field isolates","volume":"165","author":"Rowe","year":"2000"},{"key":"b13","series-title":"Nat Med","first-page":"314","article-title":"RIFINs are adhesins implicated in severe Plasmodium falciparum malaria","volume":"21","author":"Goel","year":"2015"},{"key":"b14","series-title":"Cell Host Microbe","first-page":"81","article-title":"STEVOR is a Plasmodium falciparum erythrocyte binding protein that mediates merozoite invasion and rosetting","volume":"16","author":"Niang","year":"2014"},{"key":"b15","series-title":"Blood","first-page":"541","article-title":"Changes in the blood group Wright antigens are associated with a mutation at amino acid 658 in human erythrocyte band 3: a site of interaction between band 3 and glycophorin A under certain conditions","volume":"85","author":"Bruce","year":"1995"},{"key":"b16","series-title":"Investigation of Host Red Blood Cell Receptors Essential for Rosetting in Severe Plasmodium falciparum Malaria","author":"McQuaid","year":"2019"},{"key":"b17","series-title":"J Biol Chem","first-page":"1992","article-title":"The contribution of sialic acid to the surface charge of the erythrocyte","volume":"237","author":"Eylar","year":"1962"},{"key":"b18","series-title":"Science","first-page":"eaam6393","article-title":"Resistance to malaria through structural variation of red blood cell invasion receptors","volume":"356","author":"Leffler","year":"2017"},{"key":"b19","series-title":"Blut","first-page":"33","article-title":"The Dantu erythrocyte phenotype of the NE variety: II. 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Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/110\/3\/article-p436.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/110\/3\/article-p436.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/110\/3\/article-p436.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T13:57:32Z","timestamp":1709733452000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/110\/3\/article-p436.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,6]]},"references-count":36,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.23-0347","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,6]]}}}