{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:48:36Z","timestamp":1786996116728,"version":"build-2736575974"},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,6]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Dengue virus (DENV) is the etiological agent of dengue fever (DF), which is among the most prevalent vector-borne diseases in the tropics. In 2022, the Colombian health surveillance system reported more than 69,000 cases of DF. As part of a hospital-based fever surveillance study, acute-phase sera were collected from 4,545 patients with suspected dengue between 2020 and 2023 in three municipalities of Colombia. Combined reverse transcription\u2013polymerase chain reaction and antigen rapid testing confirmed that 376 patients (8.3%) had DF. The virus was isolated in cell culture from 166 of these patients (44.1%), and genome sequencing was performed successfully on 122 (73.5%). Three DENV serotypes (1, 2, and 3) were identified. Phylogenetic analyses of the DENV-2 sequences revealed that 42 of 50 of the isolates (84%) belonged to the DENV-2 cosmopolitan genotype lineage, clustering with sequences from Asia, Peru, and Brazil. We report the detection, isolation, and whole-genome sequencing (11 Kb) of the DENV-2 cosmopolitan genotype and its recent introduction to Colombia.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0375","type":"journal-article","created":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T20:18:08Z","timestamp":1696882688000},"page":"1298-1302","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of Dengue Virus Serotype 2 Cosmopolitan Genotype Circulating in Colombia"],"prefix":"10.4269","volume":"109","author":[{"given":"Karl A.","family":"Ciuoderis","sequence":"first","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Usuga","sequence":"additional","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Moreno","sequence":"additional","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura S.","family":"Perez-Restrepo","sequence":"additional","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Y.","family":"Fl\u00f3rez","sequence":"additional","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Cardona","sequence":"additional","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin A.","family":"Cloherty","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research, Abbott Diagnostics, Abbott Park, Illinois;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Berg","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research, Abbott Diagnostics, Abbott Park, Illinois;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan P.","family":"Hernandez-Ortiz","sequence":"additional","affiliation":[{"name":"GHI One Health Colombia, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"},{"name":"Department of Materials and Nanotechnology, Universidad Nacional de Colombia, Medell\u00edn, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge E.","family":"Osorio","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin;"},{"name":"Global Health Institute, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"West J Emerg Med","first-page":"671","article-title":"Dengue, zika and chikungunya: emerging arboviruses in the new world","volume":"17","author":"Patterson","year":"2016"},{"key":"b2","series-title":"BMC Infect Dis","first-page":"466","article-title":"Epidemiology and genetic diversity of circulating dengue viruses in Medellin, Colombia: a fever surveillance study","volume":"20","author":"Lim","year":"2020"},{"key":"b3","series-title":"PLoS Negl Trop Dis","first-page":"e0003499","article-title":"Epidemiological trends of dengue disease in Colombia (2000\u20132011): a systematic review","volume":"9","author":"Villar","year":"2015"},{"key":"b4","year":"2022"},{"key":"b5","series-title":"Arch Virol","first-page":"224","article-title":"Renaming of the genus Flavivirus to Orthoflavivirus and extension of binomial species names within the family Flaviviridae","volume":"168","author":"Postler","year":"2023"},{"key":"b6","series-title":"Nature","first-page":"504","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013"},{"key":"b7","series-title":"Biology (Basel)","first-page":"941","article-title":"Modeling dengue immune responses mediated by antibodies: a qualitative study","volume":"10","author":"Sebayang","year":"2021"},{"key":"b8","series-title":"PeerJ","first-page":"e2326","article-title":"Analysis of genotype diversity and evolution of Dengue virus serotype 2 using complete genomes","volume":"4","author":"Waman","year":"2016"},{"key":"b9","series-title":"Virology","first-page":"244","article-title":"Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas","volume":"230","author":"Rico-Hesse","year":"1997"},{"key":"b10","series-title":"Trop. 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