{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:49:37Z","timestamp":1787042977025,"version":"build-2736575974"},"reference-count":34,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Melioidosis, infection caused by <jats:italic>Burkholderia pseudomallei<\/jats:italic>, is characterized by robust innate immune responses. We have previously reported associations of <jats:italic>TLR1<\/jats:italic> single nucleotide missense variant rs76600635 with mortality and of <jats:italic>TLR5<\/jats:italic> nonsense variant rs5744168 with both bacteremia and mortality in single-center studies of patients with melioidosis in northeastern Thailand. The objective of this study was to externally validate the associations of rs76600635 and rs5744168 with bacteremia and mortality in a large multicenter cohort of melioidosis patients. We genotyped rs76600635 and rs5744168 in 1,338 melioidosis patients enrolled in a prospective parent cohort study conducted at nine hospitals in northeastern Thailand. The genotype frequencies of rs76600635 did not differ by bacteremia status (<jats:italic>P<\/jats:italic> = 0.27) or 28-day mortality (<jats:italic>P<\/jats:italic> = 0.84). The genotype frequencies of rs5744168 did not differ by either bacteremia status (<jats:italic>P<\/jats:italic> = 0.46) or 28-day mortality (<jats:italic>P<\/jats:italic> = 0.10). Assuming a dominant genetic model, there was no association of the rs76600635 variant with bacteremia (adjusted odds ratio [OR], 0.75; 95% CI, 0.54\u20131.04, <jats:italic>P<\/jats:italic> = 0.08) or 28-day mortality (adjusted OR, 0.96; 95% CI, 0.71\u20131.28, <jats:italic>P<\/jats:italic> = 0.77). There was no association of the rs5744168 variant with bacteremia (adjusted OR, 1.24; 95% CI, 0.76\u20132.03, <jats:italic>P<\/jats:italic> = 0.39) or 28-day mortality (adjusted OR, 1.22; 95% CI, 0.83\u20131.79, <jats:italic>P<\/jats:italic> = 0.21). There was also no association of either variant with 1-year mortality. We conclude that in a large multicenter cohort of patients hospitalized with melioidosis in northeastern Thailand, neither <jats:italic>TLR1<\/jats:italic> missense variant rs76600635 nor <jats:italic>TLR5<\/jats:italic> nonsense variant rs5744168 is associated with bacteremia or mortality.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0381","type":"journal-article","created":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T15:19:14Z","timestamp":1714576754000},"page":"994-998","source":"Crossref","is-referenced-by-count":2,"title":["Lack of Association of TLR1 and TLR5 Coding Variants with Mortality in a Large Multicenter Cohort of Melioidosis Patients"],"prefix":"10.4269","volume":"110","author":[{"given":"Thatcha","family":"Yimthin","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"},{"name":"Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland;"},{"name":"Graduate School for Cellular and Biomedical Sciences (GCB), University of Bern, Bern, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungnapa","family":"Phunpang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelton W.","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington, Seattle, Washington;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekkachai","family":"Thiansukhon","sequence":"additional","affiliation":[{"name":"Department of Medicine, Udon Thani Hospital, Udon Thani, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seksan","family":"Chaisuksant","sequence":"additional","affiliation":[{"name":"Department of Medicine, Khon Kaen Regional Hospital, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ploenchan","family":"Chetchotisakd","sequence":"additional","affiliation":[{"name":"Department of Medicine, Srinagarind Hospital, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kittisak","family":"Tanwisaid","sequence":"additional","affiliation":[{"name":"Department of Medicine, Nakhon Phanom Hospital, Nakhon Phanom, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somchai","family":"Chuananont","sequence":"additional","affiliation":[{"name":"Department of Medicine, Nakhon Phanom Hospital, Nakhon Phanom, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chumpol","family":"Morakot","sequence":"additional","affiliation":[{"name":"Department of Medicine, Mukdahan Hospital, Mukdahan, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narongchai","family":"Sangsa","sequence":"additional","affiliation":[{"name":"Department of Medicine, Roi Et Hospital, Roi Et, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirayut","family":"Silakun","sequence":"additional","affiliation":[{"name":"Department of Medicine, Buriram Hospital, Buriram, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunee","family":"Chayangsu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surin Hospital, Surin, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noppol","family":"Buasi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Sisaket Hospital, Sisaket, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganjana","family":"Lertmemongkolchai","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Associated Medical Science, Chiang Mai University, Chiang Mai, Thailand;"},{"name":"The Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narisara","family":"Chantratita","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"},{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Eoin","family":"West","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;"},{"name":"Department of Medicine, University of Washington, Seattle, Washington;"},{"name":"Department Global Health, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Nat Microbiol","first-page":"15008","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis","volume":"1","author":"Limmathurotsakul","year":"2016"},{"key":"b2","series-title":"Lancet Reg Health Southeast Asia","first-page":"100118","article-title":"Characteristics and one year outcomes of melioidosis patients in northeastern Thailand: A prospective, multicenter cohort 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