{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T01:36:05Z","timestamp":1767836165502,"version":"3.49.0"},"reference-count":49,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Recent omics studies (cytogenomics, genomics, and transcriptomics) have provided extensive information on Chagas disease (CD) vectors. Here, we conduct a review of proteomic and metabolomic studies on these insect vectors. We address proteomic studies in triatomines, focusing on the identification and characterization of proteins in their saliva, midgut, and salivary glands, emphasizing their role in blood feeding by providing vasodilatory and anticoagulant substances, along with anti-inflammatory and antiplatelet activities. In addition, the review investigates protein diversity in different triatomine species, highlighting the importance of proteomic analysis in understanding adaptation to hematophagy, genetic evolution, and vector defense mechanisms. Metabolomic studies on CD vectors have revealed crucial information about their biology because metabolites correlate with the presence of <jats:italic>Trypanosoma cruzi<\/jats:italic> (<jats:italic>T. cruzi<\/jats:italic>), suggesting roles in the parasite\u2013vector interaction. Additionally, some studies have predicted <jats:italic>T. cruzi<\/jats:italic> infection and observed altered metabolomes after feeding on infected blood, providing information about vector exposure to the parasite.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0395","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T17:39:44Z","timestamp":1745343584000},"page":"18-24","source":"Crossref","is-referenced-by-count":1,"title":["Omics Tools Applied to the Study of Chagas Disease Vectors: Proteomics and Metabolomics"],"prefix":"10.4269","volume":"113","author":[{"given":"Kelly Cristine","family":"Borsatto","sequence":"first","affiliation":[{"name":"Centro Multiusu\u00e1rio de Inova\u00e7\u00e3o Biomolecular, Departamento de F\u00edsica, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias Letras e Ci\u00eancias Exatas, S\u00e3o Jos\u00e9 do Rio Preto, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika Aparecida","family":"Coronado","sequence":"additional","affiliation":[{"name":"Institute of Biologic Information Processing (IB-7: Structural Biochemistry), Forschungszentrum J\u00fclich, J\u00fclich, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cleber","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Nacional e Internacional de Refer\u00eancia em Taxonomia de Triatom\u00edneos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raghuvir Krishnaswamy","family":"Arni","sequence":"additional","affiliation":[{"name":"Centro Multiusu\u00e1rio de Inova\u00e7\u00e3o Biomolecular, Departamento de F\u00edsica, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias Letras e Ci\u00eancias Exatas, S\u00e3o Jos\u00e9 do Rio Preto, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaio Cesar","family":"Chaboli Alevi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Nacional e Internacional de Refer\u00eancia em Taxonomia de Triatom\u00edneos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","article-title":"Parasites\u2014American Trypanosomiasis (Also Known as Chagas Disease)","year":"2023"},{"key":"b2","series-title":"Chagas Disease (American Trypanosomiasis)","year":"2024"},{"key":"b3","series-title":"Insects","first-page":"591","article-title":"Triatoma guazu Lent and Wygodzinsky is a junior synonym of Triatoma williami Galv\u00e3o, Souza and Lima","volume":"13","author":"Oliveira Correia","year":"2022"},{"key":"b4","series-title":"Zookeys","first-page":"203","article-title":"Panstrongylus noireaui, a remarkable new species of Triatominae (Hemiptera, 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of saliva components from the hematophagous reduviid Triatoma pallidipennis","volume":"162","author":"Hern\u00e1ndez-Vargas","year":"2017"},{"key":"b22","series-title":"J Proteome Res","first-page":"669","article-title":"Insight into the salivary transcriptome and proteome of Dipetalogaster maxima research articles","volume":"10","author":"Assump\u00e7\u00e3o","year":"2011"},{"key":"b23","series-title":"J Proteomics","first-page":"47","article-title":"Exploring the molecular complexity of Triatoma dimidiata sialome","volume":"174","author":"Santiago","year":"2018"},{"key":"b24","series-title":"J Proteomics","first-page":"1652","article-title":"2-DE-based proteomic investigation of the saliva of the Amazonian triatomine vectors of Chagas disease: Rhodnius brethesi and Rhodnius robustus","volume":"74","author":"Costa","year":"2011"},{"key":"b25","series-title":"Insect Biochem Mol Biol","first-page":"83","article-title":"Comparative proteomic analysis of the saliva of the Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri triatomines reveals a high interespecific functional biodiversity","volume":"71","author":"Montandon","year":"2016"},{"key":"b26","series-title":"Front Physiol","first-page":"1051","article-title":"Genome wide mapping of peptidases in Rhodnius prolixus: Identification of protease gene duplications, horizontally transferred proteases and analysis of peptidase A1 structures, with considerations on their role in the evolution of hematophagy in triatominae","volume":"8","author":"Henriques","year":"2017"},{"key":"b27","series-title":"PLoS Negl Trop Dis","first-page":"e2594","article-title":"An insight into the transcriptome of the digestive tract of the bloodsucking bug, Rhodnius prolixus","volume":"8","author":"Ribeiro","year":"2014"},{"key":"b28","series-title":"J Insect Physiol","first-page":"108","article-title":"Proteomic analysis of the kissing bug Rhodnius prolixus 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interactions","volume":"53","author":"Garcia","year":"2007"},{"key":"b45","series-title":"Insect Molecular Biology and Biochemistry","first-page":"365","author":"Terra","year":"2012"},{"key":"b46","series-title":"Front Cell Infect Microbiol","first-page":"798924","article-title":"An integrative sialomic analysis reveals molecules from Triatoma sordida (Hemiptera: Reduviidae)","volume":"11","author":"Pra\u00e7a","year":"2022"},{"key":"b47","series-title":"Encyclopedia of Bioinformatics and Computational Biology: ABC of Bioinformatics","first-page":"396","author":"Dayalan","year":"2019"},{"key":"b48","series-title":"PLoS One","first-page":"e77283","article-title":"Metabolic signatures of triatomine vectors of Trypanosoma cruzi unveiled by metabolomics","volume":"8","author":"Antunes","year":"2013"},{"key":"b49","series-title":"Comput Struct Biotechnol J","first-page":"3051","article-title":"Metabolites as predictive biomarkers for Trypanosoma cruzi exposure in triatomine bugs","volume":"19","author":"Eberhard","year":"2021"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/113\/1\/article-p18.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/113\/1\/article-p18.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/113\/1\/article-p18.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T13:50:18Z","timestamp":1751464218000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/113\/1\/article-p18.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":49,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.23-0395","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,2]]}}}