{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:52:08Z","timestamp":1784591528337,"version":"3.55.0"},"reference-count":35,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Triatomines are insects of great importance for public health, as they are vectors of protozoan <jats:italic>Trypanosoma cruzi<\/jats:italic>, the etiological agent of Chagas disease. The genus <jats:italic>Triatoma<\/jats:italic> is the most representative in number of species and one of the most important from an epidemiological point of view. The <jats:italic>Triatoma pseudomaculata<\/jats:italic> subcomplex (composed of the species <jats:italic>Triatoma arthurneivai<\/jats:italic>, <jats:italic>T. pseudomaculata<\/jats:italic>, and <jats:italic>T. wygodzinskyi<\/jats:italic> [from the old subcomplex <jats:italic>Triatoma maculata<\/jats:italic>] and <jats:italic>Triatoma baratai<\/jats:italic>, <jats:italic>Triatoma costalimai<\/jats:italic>, <jats:italic>Triatoma deaneorum<\/jats:italic>, <jats:italic>Triatoma jatai<\/jats:italic>, and <jats:italic>Triatoma williami<\/jats:italic> [from the old <jats:italic>Triatoma matogrossensis<\/jats:italic> subcomplex]) was recently proposed based on chromosomal data. Thus, considering that the experimental crossings helped with systematic questions about triatomines, we performed crosses between the species of the <jats:italic>T. pseudomaculata<\/jats:italic> subcomplex. Thus, we crossed <jats:italic>T. pseudomaculata<\/jats:italic> and <jats:italic>Triatoma costalimai<\/jats:italic> to assess the possible presence of interspecific genomic and reproductive compatibility. In addition, based on the molecular data available on GenBank, we performed a phylogenetic systematics study to assess the evolutionary relationship of the species of the <jats:italic>T. pseudomaculata<\/jats:italic> subcomplex. Experimental crosses did not result in hybrids, demonstrating that there is a prezygotic barrier established between these species. Based on the above, we confirmed the specific status of <jats:italic>T. pseudomaculata<\/jats:italic> and <jats:italic>T. costalimai<\/jats:italic> based on the biological species concept. Furthermore, the reproductive incompatibility associated with the phylogenetic systematic analyses demonstrate that <jats:italic>T. costalimai<\/jats:italic> does not belong to the <jats:italic>T. pseudomaculata<\/jats:italic> subcomplex. Thus, we suggest that the sister species <jats:italic>T. costalimai<\/jats:italic> and <jats:italic>T. jatai<\/jats:italic> should be grouped into a new subcomplex called <jats:italic>T. costalimai<\/jats:italic>.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0415","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T13:26:49Z","timestamp":1739885209000},"page":"970-974","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of the Evolutionary Relationship between Species of the New Triatoma pseudomaculata Subcomplex (Hemiptera, Triatominae) and Proposal for the Creation of the Triatoma costalimai Subcomplex"],"prefix":"10.4269","volume":"112","author":[{"given":"Isabella","family":"da Silva Masarin","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Botucatu, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jader","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Entomologia em Sa\u00fade P\u00fablica, Departamento de Epidemiologia, Faculdade de Sa\u00fade P\u00fablica, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yago","family":"Visinho dos Reis","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Botucatu, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Ravazi","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Botucatu, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Fernandez Madeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Letras e Ci\u00eancias Exatas, S\u00e3o Jos\u00e9 do Rio Preto, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samanta","family":"Cristina Antoniassi Fernandes Tadin","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Botucatu, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleber","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Nacional e Internacional de Refer\u00eancia em Taxonomia de Triatom\u00edneos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Terc\u00edlia Vilela de Azeredo-Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Letras e Ci\u00eancias Exatas, S\u00e3o Jos\u00e9 do Rio Preto, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Aristeu","family":"da Rosa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Faculdade de Ci\u00eancias Farmac\u00eauticas, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaio Cesar Chaboli","family":"Alevi","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Instituto de Bioci\u00eancias, Botucatu, Brazil;"},{"name":"Laborat\u00f3rio Nacional e Internacional de Refer\u00eancia em Taxonomia de Triatom\u00edneos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","year":"2023"},{"key":"b2","series-title":"Pathogens","first-page":"1627","article-title":"Trends in taxonomy of Chagas disease vectors (Hemiptera, Reduviidae, Triatominae): From Linnaean to integrative taxonomy","volume":"10","author":"Alevi","year":"2021"},{"key":"b3","series-title":"Insects","first-page":"591","article-title":"Triatoma guazu Lent and Wygodzinsky is a junior synonym of Triatoma williami Galv\u00e3o, Souza and Lima","volume":"13","author":"Oliveira Correia","year":"2022"},{"key":"b4","series-title":"ZooKeys","first-page":"203","article-title":"Panstrongylus noireaui, a remarkable new species of Triatominae (Hemiptera, Reduviidae) from Bolivia","volume":"1104","author":"Gil-Santana","year":"2022"},{"key":"b5","series-title":"Insects","first-page":"331","article-title":"Triatoma yelapensis sp. nov. 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