{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T11:11:23Z","timestamp":1769339483687,"version":"3.49.0"},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>No specific treatment has been developed for severe fever with thrombocytopenia syndrome (SFTS). However, the prognosis can improve with early plasma exchange. Therefore, rapid and accurate detection of SFTS virus is important for diagnosis and prognosis. Direct real-time reverse transcription polymerase chain reaction (RT-PCR) testing is easier and more time-efficient than conventional real-time RT-PCR. Our study compared direct real-time RT-PCR efficiency without the RNA extraction and purification of conventional real-time RT-PCR. Samples were collected from 18 patients with SFTS and five without SFTS. A strong correlation (<jats:italic>r<\/jats:italic> = 0.774, 95% CI: 0.652\u20130.857, <jats:italic>P<\/jats:italic> &lt;0.01) was found between conventional and direct real-time RT-PCR assays. Direct real-time RT-PCR showed 84.4% sensitivity and 92.0% specificity for viral detection. Direct real-time RT-PCR is an effective diagnostic tool for patients with acute phase SFTS, but further optimization is required for viral detection.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0541","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T17:36:38Z","timestamp":1718732198000},"page":"429-432","source":"Crossref","is-referenced-by-count":2,"title":["Rapid Detection of Severe Fever with Thrombocytopenia Syndrome Virus in the Acute Phase of Infection by Direct Real-Time Reverse Transcription without RNA Extraction"],"prefix":"10.4269","volume":"111","author":[{"given":"Takehiro","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Infection Control Center, Oita University Hospital, Oita, Japan;"},{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takaaki","family":"Yahiro","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"},{"name":"Department of Advanced Medical Sciences, Oita University Faculty of Medicine, Oita, Japan;"},{"name":"Research Center for Global and Local Infectious Diseases, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuma","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Takenaka","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalino","family":"Demetria","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakirul","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Kimitsuki","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuzo","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazufumi","family":"Hiramatsu","sequence":"additional","affiliation":[{"name":"Infection Control Center, Oita University Hospital, Oita, Japan;"},{"name":"Research Center for Global and Local Infectious Diseases, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Nishizono","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;"},{"name":"Research Center for Global and Local Infectious Diseases, Oita, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"J Infect Chemother","first-page":"773","article-title":"Pathophysiology of severe fever with thrombocytopenia syndrome and development of specific antiviral therapy","volume":"24","author":"Saijo","year":"2018"},{"key":"b2","series-title":"J Infect Dis","first-page":"816","article-title":"The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan","volume":"209","author":"Takahashi","year":"2014"},{"key":"b3","series-title":"PLoS One","first-page":"e0165207","article-title":"Epidemiological and clinical features of severe fever 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Health","first-page":"17","article-title":"Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples","volume":"51","author":"Demetria","year":"2023"},{"key":"b9","series-title":"Bone Marrow Transplant","first-page":"452","article-title":"Investigation of the freely available easy-to-use software \u201cEZR\u201d for medical statistics","volume":"48","author":"Kanda","year":"2013"},{"key":"b10","series-title":"Jpn J Infect Dis","first-page":"465","article-title":"An ultra-rapid real-time RT-PCR method for detecting human orthopneumovirus using PCR1100","volume":"73","author":"Shirato","year":"2020"},{"key":"b11","series-title":"Jpn J Infect Dis","first-page":"29","article-title":"An ultra-rapid real-time RT-PCR method using the PCR1100 to detect severe acute respiratory syndrome coronavirus-2","volume":"74","author":"Shirato","year":"2021"},{"key":"b12","series-title":"Genes (Basel)","first-page":"1","article-title":"Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler","volume":"11","author":"Wee","year":"2020"},{"key":"b13","series-title":"Am J Trop Med Hyg","first-page":"1534","article-title":"A direct from blood\/plasma reverse transcription\u2013polymerase chain reaction for dengue virus detection in point-of-care settings","volume":"100","author":"Mehta","year":"2019"},{"key":"b14","series-title":"Nat Commun","first-page":"3328","article-title":"Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infection","volume":"9","author":"Song","year":"2018"},{"key":"b15","series-title":"J Clin Virol","first-page":"48","article-title":"Early diagnosis of novel SFTS bunyavirus infection by quantitative real-time RT-PCR assay","volume":"53","author":"Sun","year":"2012"},{"key":"b16","series-title":"Sci Rep","first-page":"16764","article-title":"Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: A prospective study","volume":"11","author":"Jalal","year":"2021"},{"key":"b17","series-title":"J Clin Virol","first-page":"57","article-title":"Kinetics of viral load and cytokines in severe fever with thrombocytopenia syndrome","volume":"101","author":"Kwon","year":"2018"},{"key":"b18","series-title":"J Clin Virol","first-page":"123","article-title":"The prospective evaluation of viral loads in patients with severe fever with thrombocytopenia syndrome","volume":"78","author":"Yang","year":"2016"},{"key":"b19","series-title":"Emerg Infect Dis","first-page":"1","article-title":"Severe fever with thrombocytopenia syndrome, Shandong Province, China, 2011","volume":"20","author":"Wen","year":"2014"}],"container-title":["The American Journal of Tropical Medicine and 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