{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T12:44:03Z","timestamp":1752669843036,"version":"3.40.4"},"reference-count":33,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>This study aimed to compare the effectiveness of three DNA extraction methods: the GF-1 Blood DNA Extraction Kit (GF-1 BD Kit), which employs a spin column along with lysing and washing buffers; the tris-ethylenediaminetetraacetic acid and proteinase K (TE-pK) method, which utilizes a combination of TE buffer and proteinase K for cell lysis; and DNAzol<jats:sup>\u00ae<\/jats:sup> Direct (DN 131), a single reagent combined with heating for the extraction process. <jats:italic>Plasmodium falciparum<\/jats:italic> DNA was extracted from both whole blood and dried blook spots (DBSs), with consideration of DNA concentration, purity, cost, time requirement, and limit of parasite detection (LOD) for each method. The target gene in this study was <jats:italic>18S rRNA<\/jats:italic>, resulting in a 395-bp product using specific primers. In the comparative analysis, the DN 131 method yielded significantly higher DNA quantities from whole blood and DBSs than the GF-1 BD Kit and TE-pK methods. In addition, the DNA purity obtained from whole blood and DBSs using the GF-1 BD Kit significantly exceeded that obtained using the TE-pK and DN 131 methods. For LOD, the whole blood extracted using the DN 131, GF-1 BD Kit, and TE-pK methods revealed 0.012, 0.012, and 1.6 parasites\/\u00b5L, respectively. In the case of DBSs, the LODs for the DN 131, GF-1 BD Kit, and TE-pK methods were 1.6, 8, and 200 parasites\/\u00b5L, respectively. The results revealed that the TE-pK method was the most cost-effective, whereas the DN 131 method showed the simplest protocol. These findings offer alternative approaches for extracting <jats:italic>Plasmodium<\/jats:italic> DNA that are particularly well-suited for large-scale studies conducted in resource-limited settings.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0612","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T16:31:20Z","timestamp":1705422680000},"page":"220-227","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of Three Methods to Extract Plasmodium falciparum DNA from Whole Blood and Dried Blood Spots"],"prefix":"10.4269","volume":"110","author":[{"given":"Thaksaporn","family":"Thongseesuksai","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thidarut","family":"Boonmars","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Porntip","family":"Laummaunwai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"year":"2022","key":"b1"},{"key":"b2"},{"key":"b3","series-title":"Malar J","first-page":"121","article-title":"Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum\/vivax endemic area in Thailand","volume":"5","author":"Coleman","year":"2006"},{"key":"b4","series-title":"Lancet Infect Dis","first-page":"582","article-title":"The reliability of diagnostic techniques in the diagnosis and 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