{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T01:17:24Z","timestamp":1752283044588},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Asymptomatic dengue virus (DENV) infections have important public health implications but are challenging to identify. We performed a cross-sectional study of reverse transcription quantitative polymerase chain reaction on pooled sera of asymptomatic individuals from the south coast of Kenya at two time periods to identify cases of asymptomatic viremia. Among 2,460 samples tested in pools of 9 or 10, we found only one positive case (0.04% incidence). Although pooling of samples has the potential to be a cost-effective and time-efficient method for asymptomatic DENV detection, mass cross-sectional pooled testing may not provide accurate data on rates of asymptomatic infection, likely owing to a decrease in the sensitivity with pooling of samples, a short period of viremia, or testing in the absence of an outbreak.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0650","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T16:31:36Z","timestamp":1710261096000},"page":"738-740","source":"Crossref","is-referenced-by-count":5,"title":["Low Rate of Asymptomatic Dengue Infection Detected in Coastal Kenya Using Pooled Polymerase Chain Reaction Testing"],"prefix":"10.4269","volume":"110","author":[{"given":"Melanie","family":"Kiener","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nader","family":"Shayegh","sequence":"additional","affiliation":[{"name":"Howard University College of Medicine, Washington, District of Columbia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sindiso Victor","family":"Nyathi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryson Alberto","family":"Ndenga","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Kisumu, Kenya;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis Maluki","family":"Mutuku","sequence":"additional","affiliation":[{"name":"Technical University of Mombasa, Mombasa, Kenya;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelle Desiree","family":"LaBeaud","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Infectious Diseases, Stanford University School of Medicine, Stanford, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Front Immunol","first-page":"280","article-title":"Epidemiological risk factors associated with high global frequency of inapparent dengue virus infections","volume":"5","author":"Grange","year":"2014"},{"key":"b2","series-title":"PLoS Pathog","first-page":"e1006965","article-title":"Contributions from the silent majority dominate dengue virus transmission","volume":"14","author":"ten Bosch","year":"2018"},{"key":"b3","series-title":"Front Physiol","first-page":"70","article-title":"Eventual role of asymptomatic cases of dengue for the introduction and spread of dengue viruses in non-endemic 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