{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:34:33Z","timestamp":1783434873975,"version":"3.54.6"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:p>Zoonotic malaria, caused by <jats:italic>Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium coatneyi<\/jats:italic>, and <jats:italic>Plasmodium inui<\/jats:italic>, is a significant global health concern. The gold standard microscopy, while widely used for malaria diagnosis, faces limitations in differentiating between malaria species. Polymerase chain reaction (PCR), despite its accuracy, is characterized by high costs and time-consuming procedures. This study aims to develop and validate a rapid and accurate diagnostic test for detecting four simian <jats:italic>Plasmodium<\/jats:italic> species by using loop-mediated isothermal amplification (LAMP). Loop-mediated isothermal amplification is a cost-effective and faster molecular testing alternative for malaria diagnosis. The project involved designing specific primers, testing sensitivity and specificity against various parasites (including human <jats:italic>Plasmodium<\/jats:italic> species, protozoa, and helminths), and evaluating the LAMP assay using 60 macaque samples infected with simian <jats:italic>Plasmodium<\/jats:italic>. The LAMP assay exhibited a sensitivity profile enabling the detection of <jats:italic>P. knowlesi<\/jats:italic>, <jats:italic>P. coatneyi<\/jats:italic>, and <jats:italic>P. cynomolgi<\/jats:italic> across a concentration gradient from 5 \u00d7 10<jats:sup>8<\/jats:sup> down to 5 \u00d7 10<jats:sup>5<\/jats:sup> parasites\/\u00b5L. Notably, <jats:italic>P. inui<\/jats:italic> was detectable at 5 \u00d7 10<jats:sup>8<\/jats:sup> parasites\/\u00b5L. Furthermore, the specificity of the primer tailored for the four simian <jats:italic>Plasmodium<\/jats:italic> species was proven, as it produced a positive amplification exclusively for the respective target species and generated negative results for nontarget species. The results indicated that the LAMP assay is capable of detecting simian <jats:italic>Plasmodium<\/jats:italic> within a short span of 60\u2009minutes, without any false positives from other samples. This new test has the potential to revolutionize malaria diagnosis, surveillance, and control, thereby mitigating the impact of zoonotic malaria in regions of endemicity.<\/jats:p>","DOI":"10.4269\/ajtmh.23-0879","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T09:43:15Z","timestamp":1722937395000},"source":"Crossref","is-referenced-by-count":1,"title":["Loop-Mediated Isothermal Amplification for Diagnosis of Zoonotic Malaria"],"prefix":"10.4269","author":[{"given":"Meng Yee","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainaa Nadrah","family":"Sohairi","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Phone Youth","family":"Zen","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Lutfi","family":"Abdullah","sequence":"additional","affiliation":[{"name":"National Wildlife Forensic Laboratory, Ex-Situ Conservation Division, Department of Wildlife and National Parks Peninsular Malaysia, Jalan Cheras, Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/aop\/article-10.4269-ajtmh.23-0879\/article-10.4269-ajtmh.23-0879.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/aop\/article-10.4269-ajtmh.23-0879\/article-10.4269-ajtmh.23-0879.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/aop\/article-10.4269-ajtmh.23-0879\/article-10.4269-ajtmh.23-0879.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T09:43:15Z","timestamp":1722937395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/aop\/article-10.4269-ajtmh.23-0879\/article-10.4269-ajtmh.23-0879.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":0,"URL":"https:\/\/doi.org\/10.4269\/ajtmh.23-0879","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,6]]}}}