{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T05:23:54Z","timestamp":1736400234868,"version":"3.32.0"},"reference-count":43,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,8]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Large diagnostic panels allow for pathogens with high or low likelihood of causing attributable illness to be tested simultaneously. Infectious mononucleosis (IM) due to primary infection with Epstein-Barr virus (EBV) is a common cause of acute febrile illness (AFI) in case series from high-income countries, though its contribution to AFI in tropical low-income settings is unclear. As part of a case-control study using multiplex quantitative polymerase chain reaction (qPCR) diagnostics, we set out to determine if primary EBV infection was an underrecognized cause of AFI in the Peruvian Amazon. Presence of EBV DNA in whole-blood samples was equally prevalent among febrile cases and afebrile controls (34.6% [247\/714] versus 35.7% [248\/695]) and was not correlated with classic IM symptoms. Given the clear lack of clinical significance of the whole-blood PCR results, additional testing was pursued to ascertain the true prevalence of IM among cases of AFI in this population. The presence of EBV DNA in plasma, a marker of active EBV-related processes, was detected in 7% (5\/68). Anti\u2013EBNA-1 IgG, a late marker of prior infection, was tested via ELISA and detected in 4\/5 of the plasma-positive patients, thereby excluding an acute primary EBV infection in all but one patient. Infectious mononucleosis due to primary infection with EBV was not an important etiology of AFI in the Peruvian Amazon, despite high rates of initial test positivity.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0051","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T19:28:59Z","timestamp":1731439739000},"page":"200-207","source":"Crossref","is-referenced-by-count":0,"title":["Epstein-Barr Virus in the RIVERA Case-Control Study of Acute Febrile Illness: Acute Mononucleosis Nearly Absent as an Etiology in the Peruvian Amazon"],"prefix":"10.4269","volume":"112","author":[{"given":"Thomas G.","family":"Flynn","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and International Health, School of Medicine, University of Virginia, Charlottesville, Virginia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maribel","family":"Paredes Olortegui","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n Ben\u00e9fica Prisma, Investigaciones Biom\u00e9dicas, Iquitos, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul F.","family":"Garcia Bardales","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n Ben\u00e9fica Prisma, Investigaciones Biom\u00e9dicas, Iquitos, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Schiaffino","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, School of Medicine, University of Virginia, Charlottesville, Virginia;"},{"name":"Faculty of Veterinary Medicine, Universidad Peruana Cayetano Heredia, San Mart\u00edn de Porres, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tackeshy N.","family":"Pinedo Vasquez","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n Ben\u00e9fica Prisma, Investigaciones Biom\u00e9dicas, Iquitos, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner V.","family":"Shapiama L\u00f3pez","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n Ben\u00e9fica Prisma, Investigaciones Biom\u00e9dicas, Iquitos, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Pe\u00f1ataro Yori","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, School of Medicine, University of Virginia, Charlottesville, Virginia;"},{"name":"Asociaci\u00f3n Ben\u00e9fica Prisma, Investigaciones Biom\u00e9dicas, Iquitos, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar J.","family":"Ramal Asayag","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universidad Nacional de la Amazon\u00eda Peruana, Iquitos, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graciela R.","family":"Meza S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universidad Nacional de la Amazon\u00eda Peruana, Iquitos, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josh M.","family":"Colston","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, School of Medicine, University of Virginia, Charlottesville, Virginia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret N.","family":"Kosek","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, School of Medicine, University of Virginia, Charlottesville, Virginia;"},{"name":"Asociaci\u00f3n Ben\u00e9fica Prisma, Investigaciones Biom\u00e9dicas, Iquitos, Per\u00fa;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Acta Trop","first-page":"106286","article-title":"Opportunities and challenges to accurate 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