{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T08:25:04Z","timestamp":1758702304131,"version":"3.28.0"},"reference-count":21,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,6]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Melioidosis is an emerging tropical infectious disease with a rising global burden caused by the environmental bacterium <jats:italic>Burkholderia pseudomallei<\/jats:italic>. It is endemic in Southeast and South Asia, including Bangladesh. A rare aminoglycoside-susceptible <jats:italic>B. pseudomallei<\/jats:italic> isolate (Y2019) has recently been reported from a melioidosis patient in Dhaka, Bangladesh. To understand the geographical origins of Y2019, we subjected it and 10 other isolates from Bangladesh to whole-genome sequencing. In a phylogenetic tree with a global set of <jats:italic>B. pseudomallei<\/jats:italic> genomes, most Bangladeshi genomes clustered tightly within the Asian clade. In contrast, Y2019 was closely related to ST881 isolates from Sarawak, Malaysian Borneo, a gentamicin-sensitive sequence type, suggesting infection in Borneo. Y2019 also contained the same gentamicin sensitivity conferring nonsynonymous mutation in the drug efflux pump encoding the <jats:italic>amrB<\/jats:italic> gene. In the absence of a full travel history, whole-genome sequencing and bioinformatics tools have revealed the likely origin of this rare isolate.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0144","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T17:21:13Z","timestamp":1725384073000},"page":"1056-1059","source":"Crossref","is-referenced-by-count":1,"title":["Use of Comparative Genomics To Resolve an Unusual Case of Aminoglycoside Susceptibility in the Melioidosis Pathogen Burkholderia pseudomallei in Bangladesh"],"prefix":"10.4269","volume":"111","author":[{"given":"Mirjam","family":"Kaestli","sequence":"first","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saika","family":"Farook","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Shariful Alam","family":"Jilani","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaheda","family":"Anwar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanvir Ahmed","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwana","family":"Podin","sequence":"additional","affiliation":[{"name":"Institute of Health and Community Medicine, Universiti Malaysia Sarawak, Sarawak, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica R.","family":"Webb","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia;"},{"name":"Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A. B.","family":"Dance","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital\u2014Wellcome Trust Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People\u2019s Democratic Republic;"},{"name":"Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart J.","family":"Currie","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia;"},{"name":"Infectious Diseases Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Nat Rev Microbiol","first-page":"155","article-title":"Burkholderia pseudomallei and melioidosis","volume":"22","author":"Meumann","year":"2024"},{"key":"b2","series-title":"Lancet Infect 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Bangladesh","volume":"17","author":"Jilani","year":"2023"},{"key":"b7","series-title":"Pathology","first-page":"293","article-title":"An improved screening technique for isolation of Pseudomonas pseudomallei from clinical specimens","volume":"11","author":"Ashdown","year":"1979"},{"key":"b8","series-title":"PLoS Negl Trop Dis","first-page":"e2105","article-title":"Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei","volume":"7","author":"Limmathurotsakul","year":"2013"},{"key":"b9","series-title":"Am J Trop Med Hyg","first-page":"292","article-title":"Sri Lankan National Melioidosis Surveillance Program uncovers a nationwide distribution of invasive melioidosis","volume":"94","author":"Corea","year":"2016"},{"key":"b10","series-title":"Diagn Microbiol Infect Dis","first-page":"115878","article-title":"Genomics and structural insight into the masking of gentamicin-resistance in clinical Burkholderia pseudomallei strain VB29710 from 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pseudomallei","volume":"67","author":"Hall","year":"2023"},{"key":"b15","series-title":"IMC J Med Sci","article-title":"Melioidosis by aminoglycoside susceptible Burkholderia pseudomallei: First case in Bangladesh","volume":"14","author":"Farook","year":"2020"},{"key":"b16","series-title":"Open Forum Infect Dis","first-page":"ofad405","article-title":"Melioidosis in Timor-Leste: First case description and phylogenetic analysis","volume":"10","author":"Guterres","year":"2023"},{"key":"b17","series-title":"EBioMedicine","first-page":"103152","article-title":"Taking the next-gen step: Comprehensive antimicrobial resistance detection from Burkholderia pseudomallei.","volume":"63","author":"Madden","year":"2021"},{"key":"b18","series-title":"PLoS Negl Trop Dis","first-page":"e0007672","article-title":"Opportunistic pathogens and large microbial diversity detected in source-to-distribution drinking water of three remote communities in Northern 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