{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T17:57:31Z","timestamp":1784138251014,"version":"3.55.0"},"reference-count":25,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,8]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Amoebic liver abscess (ALA) is a significant public health concern in tropical countries. Traditional diagnostic methods, such as microscopy, have low sensitivity, and nested polymerase chain reaction (nPCR) has lengthy turnaround times. In this study, we aimed to evaluate the effectiveness of the recombinase polymerase amplification (RPA) assay for detecting <jats:italic>Entamoeba histolytica<\/jats:italic> in amoebic liver pus samples. The assay was tested on 150 clinical pus samples collected from suspected ALA patients in the emergency department of a tertiary care center located in Chandigarh, northern India. For comparison with RPA, nPCR was also performed on these samples. Of 150 samples, 79 (53%) tested positive for <jats:italic>E. histolytica<\/jats:italic> using the RPA assay, and similar (79; 53%) number of samples tested positive with nested PCR. In addition, sequences obtained through Sanger sequencing showed high similarity with other sequences in the National Center for Biotechnology Information database and were submitted to the database. Our findings highlight the potential of RPA as a valuable tool for the accurate diagnosis of ALA.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0269","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T11:43:39Z","timestamp":1729683819000},"page":"96-100","source":"Crossref","is-referenced-by-count":1,"title":["Rapid Detection of Amoebic Liver Abscess Using Recombinase Polymerase Amplification Assay in Resource-Limited Settings"],"prefix":"10.4269","volume":"112","author":[{"given":"Priya","family":"Datta","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Rattan","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navneet","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Kalra","sequence":"additional","affiliation":[{"name":"Department of Radio-Diagnosis and Imaging, Postgraduate Institute of Medical Education and Research, Chandigarh, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Duseja","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumeeta","family":"Khurana","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Amebic Liver Abscess","author":"Jackson-Akers","year":"2023"},{"key":"b2","series-title":"Trop Med Health","first-page":"2","article-title":"Entamoeba histolytica and amoebic liver abscess in northern Sri Lanka: A public health problem","volume":"48","author":"Tharmaratnam","year":"2020"},{"key":"b3","series-title":"World J Hepatol","first-page":"316","article-title":"Amebic liver abscess: An update","volume":"16","author":"Kumar","year":"2024"},{"key":"b4","series-title":"New Microbes New Infect","first-page":"50","article-title":"Enteric multiplex PCR panels: A new diagnostic tool for amoebic liver abscess?","volume":"18","author":"Weitzel","year":"2017"},{"key":"b5","series-title":"Parasit Vectors","first-page":"143","article-title":"Digital PCR: Modern solution to parasite diagnostics and population trait 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