{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:55Z","timestamp":1787031175275,"version":"build-2736575974"},"reference-count":34,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,5]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>Cache Valley virus (CVV), a mosquito-borne orthobunyavirus, causes epizootics in ruminants characterized by congenital malformations and fetal death in North America. Only seven human infections have been identified; limited information exists on its potential as a human teratogen. Diagnosis of CVV infections relies on the plaque reduction neutralization test (PRNT), which requires live virus, is time-consuming, and cannot differentiate between recent and past infections. To improve diagnostics for CVV, we developed an IgM antibody capture ELISA (MAC-ELISA) for detection of anti-CVV human IgM in diagnostic specimens that can be performed faster than PRNT and is specific to IgM, which is essential to determine the timing of infection. Conjointly, a cell line constitutively expressing human-murine chimeric antibody with the variable regions of monoclonal antibody CVV-17 and constant regions of human IgM was developed to provide positive control material. The new cell line produced antibody with reactivity in the assay equivalent to that of a human serum sample positive for anti-CVV IgM. Five of seven archived human specimens diagnostically confirmed as CVV positive tested positive in the MAC-ELISA, whereas 44 specimens confirmed positive for another arboviral infection tested negative, showing good initial correlation of the CVV MAC-ELISA. Two of 27 previously collected serum samples from febrile patients in Yucat\u00e1n, Mexico, who tested negative for a recent flaviviral or alphaviral infection were positive in both the MAC-ELISA and PRNT, indicating a possible recent infection with CVV or related orthobunyavirus. The MAC-ELISA described here will aid in making diagnostics more widely available for CVV in public health laboratories.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0360","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T10:47:53Z","timestamp":1732013273000},"page":"386-395","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Diagnostic IgM Antibody Capture ELISA for Detection of Anti-Cache Valley Virus Human IgM"],"prefix":"10.4269","volume":"112","author":[{"given":"Christin","family":"Goodman","sequence":"first","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan A.","family":"Powers","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sierra R.","family":"Mikula","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly R.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brad J.","family":"Biggerstaff","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Fitzpatrick","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda J.","family":"Panella","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Machain-Williams","sequence":"additional","affiliation":[{"name":"Unidad Profesional Interdisciplinaria de Ingenier\u00eda Palenque (UPIIP), Instituto Polit\u00e9cnico Nacional, Palenque, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SeungHwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, Seoul National University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda E.","family":"Calvert","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Science","first-page":"1187","article-title":"Cache Valley virus, a previously undescribed mosquito-borne agent","volume":"130","author":"Holden","year":"1959"},{"key":"b2","series-title":"Am J Trop Med Hyg","first-page":"429","article-title":"Distribution of Bunyamwera serogroup viruses in North America, 1956\u20131984","volume":"35","author":"Calisher","year":"1986"},{"key":"b3","series-title":"Virus Genes","first-page":"176","article-title":"Nucleotide sequencing and serologic analysis of Cache Valley virus isolates from the Yucatan Peninsula of Mexico","volume":"45","author":"Blitvich","year":"2012"},{"key":"b4","series-title":"Emerg Infect Dis","first-page":"1325","article-title":"Maguari virus associated with human disease","volume":"23","author":"Groseth","year":"2017"},{"key":"b5","series-title":"J Med 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