{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T03:30:42Z","timestamp":1789183842822,"version":"build-2803163510"},"reference-count":43,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,8]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Chagas disease (CD), a neglected tropical disease caused by <jats:italic>Trypanosoma cruzi<\/jats:italic>, is a significant public health issue particularly in Latin America, affecting millions worldwide. Diagnosis is a challenge owing to the genetic diversity of <jats:italic>T. cruzi<\/jats:italic> and the complexities involved in selecting antigens for the detection of anti\u2013<jats:italic>T. cruzi<\/jats:italic> antibodies. This study evaluated four chimeric recombinant antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) designed to enhance diagnostic accuracy by addressing assay variability. We compared the diagnostic performance of these chimeric antigens using indirect ELISA as a diagnostic platform, with three commercial serological assays in Brazil, analyzing 100 serum samples from individuals with confirmed CD and 86 from non-infected controls. The results revealed that all assays and antigens demonstrated an area under the receiver operating characteristic curve of 100%, signifying their exceptional ability to distinguish between CD-positive and CD-negative samples. Notably, the chimeric antigens achieved 100% sensitivity, specificity, accuracy, and kappa index, equaling or surpassing the commercial assays. This research highlights the efficacy of IBMP chimeric antigens as reliable diagnostic tools for CD, suggesting their potential integration into commercial diagnostic platforms to enhance the accuracy and reliability of CD detection.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0379","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T21:23:42Z","timestamp":1730237022000},"page":"89-95","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of Four Chimeric Antigens and Commercial Serological Assays for the Diagnosis of Trypanosoma cruzi Infection"],"prefix":"10.4269","volume":"112","author":[{"given":"Nat\u00e1lia Erdens Maron","family":"Freitas","sequence":"first","affiliation":[{"name":"Advanced Public Health Laboratory, Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis Augusto Argolo","family":"Campos","sequence":"additional","affiliation":[{"name":"Advanced Public Health Laboratory, Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randrin Queiroz Viana","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Advanced Public Health Laboratory, Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Silva Santos de","family":"Jesus","sequence":"additional","affiliation":[{"name":"Advanced Public Health Laboratory, Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngelo Ant\u00f4nio Oliveira","family":"Silva","sequence":"additional","affiliation":[{"name":"Advanced Public Health Laboratory, Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane Oliveira da","family":"Mota","sequence":"additional","affiliation":[{"name":"Gon\u00e7alo Moniz Public Health Central Laboratory (LACEN-BA), Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabricio Klerynton","family":"Marchini","sequence":"additional","affiliation":[{"name":"Molecular Biology Institute of Paran\u00e1, Curitiba, Brazil;"},{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ-PR), Curitiba, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Alejandra Fiorani","family":"Celedon","sequence":"additional","affiliation":[{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Molecular Biology of Trypanosomatids Laboratory, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilson Ivo Tonin","family":"Zanchin","sequence":"additional","affiliation":[{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Structural Biology and Protein Engineering Laboratory, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz-PR), Curitiba, Brazil;"},{"name":"Integrated Translational Program in Chagas Disease from FIOCRUZ (Fio-Chagas), Oswaldo Cruz Foundation (FIOCRUZ-RJ), Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred Luciano Neves","family":"Santos","sequence":"additional","affiliation":[{"name":"Advanced Public Health Laboratory, Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil;"},{"name":"Integrated Translational Program in Chagas Disease from FIOCRUZ (Fio-Chagas), Oswaldo Cruz Foundation (FIOCRUZ-RJ), Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","year":"2024"},{"key":"b2","series-title":"Front Public Health","first-page":"166","article-title":"Chagas disease: From discovery to a worldwide health problem","volume":"7","author":"Lidani","year":"2019"},{"key":"b3","series-title":"Mem Inst Oswaldo Cruz","first-page":"e210151","article-title":"Population movements, borders, and Chagas disease","volume":"117","author":"Avaria","year":"2021"},{"key":"b4","series-title":"Infect Dis Clin North Am","first-page":"275","article-title":"American trypanosomiasis (Chagas disease)","volume":"26","author":"Rassi","year":"2012"},{"key":"b5","series-title":"Mem Inst Oswaldo Cruz","first-page":"115","article-title":"Diagnosis of Chagas disease: What has been achieved? What remains to be done with regard to diagnosis and follow up studies?","volume":"104","author":"Gomes","year":"2009"},{"key":"b6","series-title":"Am J Trop Med Hyg","first-page":"1034","article-title":"Chronic Chagas disease diagnosis: A comparative performance of commercial enzyme immunoassay tests","volume":"94","author":"Santos","year":"2016"},{"key":"b7","series-title":"Acta Trop","first-page":"38","article-title":"Trypanosoma cruzi genetic diversity: Something new for something known about Chagas disease manifestations, serodiagnosis and drug sensitivity","volume":"184","author":"Zingales","year":"2018"},{"key":"b8","series-title":"J Clin Epidemiol","first-page":"5","article-title":"Diagnostic test accuracy may vary with prevalence: Implications for evidence-based diagnosis","volume":"62","author":"Leeflang","year":"2009"},{"key":"b9","series-title":"CMAJ","first-page":"E537","article-title":"Variation of a test\u2019s sensitivity and specificity with disease prevalence","volume":"185","author":"Leeflang","year":"2013"},{"key":"b10","series-title":"Am J Trop Med Hyg","first-page":"1074","article-title":"Regional variation in the correlation of antibody and T-cell responses to Trypanosoma cruzi.","volume":"90","author":"Martin","year":"2014"},{"key":"b11","series-title":"Guidelines for the Diagnosis and Treatment of Chagas disease","year":"2019"},{"key":"b12","series-title":"Clin Vaccine Immunol","first-page":"899","article-title":"Comparison of recombinant Trypanosoma cruzi peptide mixtures versus multiepitope chimeric proteins as sensitizing antigens for immunodiagnosis","volume":"16","author":"Camussone","year":"2009"},{"key":"b13","series-title":"PLoS One","first-page":"e0161100","article-title":"Performance assessment of four chimeric Trypanosoma cruzi antigens based on antigen-antibody detection for diagnosis of chronic Chagas disease","volume":"11","author":"Santos","year":"2016"},{"key":"b14","series-title":"Biosensors (Basel)","first-page":"289","article-title":"Stability assessment of four chimeric proteins for human Chagas disease immunodiagnosis","volume":"11","author":"Celedon","year":"2021"},{"key":"b15","series-title":"PLoS Negl Trop Dis","first-page":"e0005433","article-title":"Accuracy of chimeric proteins in the serological diagnosis of chronic Chagas disease \u2013 A Phase II study","volume":"11","author":"Santos","year":"2017"},{"key":"b16","series-title":"J Clin Microbiol","first-page":"2934","article-title":"Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays","volume":"55","author":"Santos","year":"2017"},{"key":"b17","series-title":"Am J Trop Med Hyg","first-page":"1174","article-title":"Highly accurate chimeric proteins for the serological diagnosis of chronic Chagas disease: A latent class analysis","volume":"99","author":"Santos","year":"2018"},{"key":"b18","series-title":"BMC Infect Dis","first-page":"251","article-title":"Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting","volume":"19","author":"Dopico","year":"2019"},{"key":"b19","series-title":"PLoS One","first-page":"e0215623","article-title":"Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries","volume":"14","author":"Del-Rei","year":"2019"},{"key":"b20","series-title":"Biosens Bioelectron","first-page":"112573","article-title":"Impedimetric immunosensor for rapid and simultaneous detection of Chagas and visceral leishmaniasis for point of care diagnosis","volume":"169","author":"Cordeiro","year":"2020"},{"key":"b21","series-title":"Transfusion","first-page":"2701","article-title":"Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens","volume":"61","author":"Santos","year":"2021"},{"key":"b22","series-title":"Front Med","first-page":"852864","article-title":"Performance of chimeric Trypanosoma cruzi antigens in serological screening for Chagas disease in blood banks","volume":"9","author":"Santos","year":"2022"},{"key":"b23","series-title":"J Clin Microbiol","first-page":"e00762-19","article-title":"Cross-reactivity using chimeric Trypanosoma cruzi antigens: Diagnostic performance in settings where Chagas disease and American cutaneous or visceral leishmaniasis are coendemic","volume":"57","author":"Daltro","year":"2019"},{"key":"b24","author":"Dean","year":"2013"},{"key":"b25","series-title":"Science","first-page":"1285","article-title":"Measuring the accuracy of diagnostic systems","volume":"240","author":"Swets","year":"1988"},{"key":"b26","series-title":"\u00c9valuation des M\u00e9thodes d\u2019Analyse Appliqu\u00e9es aux Sciences de la Vie et de la Sant\u00e9 - Biostatistique","first-page":"49","author":"Ouchchane","year":"2009"},{"key":"b27","series-title":"J Insect Sci","first-page":"34","article-title":"Overlapping confidence intervals or standard error intervals: What do they mean in terms of statistical significance?","volume":"3","author":"Payton","year":"2003"},{"key":"b28","series-title":"Biometrics","first-page":"159","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis","year":"1977"},{"key":"b29","series-title":"BMJ","first-page":"h5527","article-title":"STARD 2015: An updated list of essential items for reporting diagnostic accuracy studies","volume":"351","author":"Bossuyt","year":"2015"},{"key":"b30","series-title":"BioMed Res Int","first-page":"1803515","article-title":"Development of a new lateral flow assay based on IBMP-8.1 and IBMP-8.4 chimeric antigens to diagnose Chagas disease","volume":"2020","author":"Silva","year":"2020"},{"key":"b31","series-title":"Trop Med Int Health","first-page":"1103","article-title":"Performance of different Trypanosoma cruzi antigens in the diagnosis of Chagas disease in patients with American cutaneous leishmaniasis from a co-endemic region in Argentina","volume":"18","author":"Vega Benedetti","year":"2013"},{"key":"b32","series-title":"Clin Vaccine Immunol","first-page":"1045","article-title":"Evaluation of serological tests to identify Trypanosoma cruzi infection in humans and determine cross-reactivity with Trypanosoma rangeli and Leishmania spp","volume":"14","author":"Caballero","year":"2007"},{"key":"b33","series-title":"Parasit Vectors","first-page":"20","article-title":"Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera","volume":"1","author":"de Moraes","year":"2008"},{"key":"b34","series-title":"Infect Genet Evol","first-page":"457","article-title":"Detection of Chagas infections using Trypanosoma evansi crude antigen demonstrates high cross-reactions with Trypanosoma cruzi.","volume":"7","author":"Desquesnes","year":"2007"},{"key":"b35","series-title":"Emerg Infect Dis","first-page":"2088","article-title":"Non-Leishmania parasite in fatal visceral leishmaniasis-like disease, Brazil","volume":"25","author":"Maruyama","year":"2019"},{"key":"b36","series-title":"Diagnostics (Basel)","first-page":"3470","article-title":"Assessment of cross-reactivity of chimeric Trypanosoma cruzi antigens with Crithidia sp. LVH-60A: Implications for accurate diagnostics","volume":"13","author":"Santos","year":"2023"},{"key":"b37","series-title":"Mem Inst Oswaldo Cruz","first-page":"e200634","article-title":"Genomics and functional genomics in Leishmania and Trypanosoma cruzi: Statuses, challenges and perspectives","volume":"116","author":"Bartholomeu","year":"2021"},{"key":"b38","series-title":"Genet Mol Biol","first-page":"1","article-title":"Trypanosomatid comparative genomics: Contributions to the study of parasite biology and different parasitic diseases","volume":"35","author":"Teixeira","year":"2012"},{"key":"b39","series-title":"ACP J Club","first-page":"A17","article-title":"On some clinically useful measures of the accuracy of diagnostic tests","volume":"129","author":"Sackett","year":"1998"},{"key":"b40","series-title":"Med Clin (Barc)","first-page":"348","article-title":"Analysis of clinical decisions","volume":"94","author":"Rodr\u00edguez Artalejo","year":"1990"},{"key":"b41","series-title":"J Clin Epidemiol","first-page":"1129","article-title":"The diagnostic odds ratio: A single indicator of test performance","volume":"56","author":"Glas","year":"2003"},{"key":"b42","series-title":"Pathogens","first-page":"1222","article-title":"Seroepidemiological survey of chronic Chagas disease in a rural community in Southern Bahia, Brazil, using recombinant chimeric antigens","volume":"12","author":"Soares","year":"2023"},{"key":"b43","series-title":"Diagnostics (Basel)","first-page":"2752","article-title":"Accuracy of diagnostic tests for the detection of Chagas disease: A systematic review and meta-analysis","volume":"12","author":"Candia-Puma","year":"2022"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/112\/1\/article-p89.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/112\/1\/article-p89.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/112\/1\/article-p89.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T13:06:35Z","timestamp":1736341595000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/112\/1\/article-p89.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,8]]},"references-count":43,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.24-0379","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,8]]}}}