{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T04:13:49Z","timestamp":1743653629120,"version":"3.40.3"},"reference-count":31,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>The socioecological conditions of Mexican regions are conducive to the spread of vector-borne diseases. Although there are established treatment guidelines for dengue and rickettsiosis, diagnosis is complicated. The objective of this work was to identify epitopes of <jats:italic>Rickettsia<\/jats:italic> and dengue virus that could be used in serology screening against vector-borne diseases. For this, epitopes with high histocompatibility complex class II binding efficiency of <jats:italic>OmpB<\/jats:italic> protein of <jats:italic>Rickettsia rickettsii<\/jats:italic> and <jats:italic>NS2B<\/jats:italic> protein of dengue virus were identified in silico through a reverse vaccinology strategy. The selected epitopes were grouped into multipeptide sequences that were synthesized and immobilized in a nitrocellulose membrane to evaluate the reactivity sera from patients previously infected with dengue or <jats:italic>Rickettsia<\/jats:italic>. The evaluation of the sequences of the <jats:italic>NS2B<\/jats:italic> and <jats:italic>OmpB<\/jats:italic> proteins was performed with 60 sera previously diagnosed as positive or negative by the respective gold standard techniques. The dot blot technique was used for the antigenic evaluation of the peptides against these serum samples. Dot blot analysis correctly identified 85% of sera positive for rickettsiosis and 75% of sera positive for dengue. Experimental evidence from multipeptide sequences suggests their potential use in the development of diagnostic tests for dengue and rickettsiosis.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0491","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T17:49:26Z","timestamp":1738086566000},"page":"909-915","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Multipeptide Sequences Identified in Silico for the Serological Detection of Antibodies against Vector-Borne Diseases"],"prefix":"10.4269","volume":"112","author":[{"given":"Carlos A.","family":"Pe\u00f1a-Bates","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar I.","family":"Lugo-Caballero","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma","family":"Pav\u00eda-Ruz","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oghenekaro","family":"Omodior","sequence":"additional","affiliation":[{"name":"Health Affairs Institute, West Virginia University, Charleston, West Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry R.","family":"Noh-Pech","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando I.","family":"Puerto-Manzano","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karla R.","family":"Dzul-Rosado","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"year":"2020","series-title":"Vector-Borne Diseases","key":"b1"},{"key":"b2","series-title":"Clin Med (Lond)","first-page":"9","article-title":"Dengue infection: Global importance, immunopathology and management","volume":"22","author":"Kularatne","year":"2022"},{"key":"b3","series-title":"Open Vet J","first-page":"165","article-title":"Direct evidence of Rickettsia typhi infection in Rhipicephalus sanguineus ticks and their canine hosts","volume":"7","author":"Dzul-Rosado","year":"2017"},{"key":"b4","series-title":"Ticks Tick Borne Dis","first-page":"101911","article-title":"Rickettsia spp. in ticks from a tropical dry forest reserve on Mexico\u2019s Pacific Coast","volume":"13","author":"Guzm\u00e1n-Cornejo","year":"2022"},{"year":"2013","article-title":"Ensayo de RT-PCR en tiempo real para DENV 1\u20134 de los CDC, para la detecci\u00f3n del virus dengue e identificaci\u00f3n de su serotipo","key":"b5"},{"key":"b6","series-title":"Methods Mol Biol","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/978-1-0716-1879-0_11","article-title":"Molecular diagnosis of dengue","volume":"2409","author":"Nunes","year":"2022"},{"key":"b7","series-title":"Vector Borne Zoonotic Dis","first-page":"93","article-title":"Comparison of commercial enzyme-linked immunosorbent assay and immunofluorescence assay for diagnosis of acute Rickettsia typhi infections","volume":"20","author":"Lokida","year":"2020"},{"key":"b8","series-title":"Am J Trop Med Hyg","first-page":"157","article-title":"Unrecognized spotted fever group rickettsiosis masquerading as dengue fever in Mexico","volume":"55","author":"Zavala-Velazquez","year":"1996"},{"key":"b9","series-title":"Infect","first-page":"259","article-title":"Contribuci\u00f3n de la prueba r\u00e1pida NS1 e IgM al diagn\u00f3stico de dengue en Colombia en el periodo pre-Zika","volume":"23","author":"Clemen","year":"2019"},{"key":"b10","series-title":"Int J Mol Sci","first-page":"1921","article-title":"Computational analysis of dengue virus envelope protein (E) reveals an epitope with flavivirus immunodiagnostic potential in peptide microarrays","volume":"20","author":"Bergamaschi","year":"2019"},{"key":"b11","series-title":"J Cell Biochem","first-page":"5869","article-title":"Identification of B- and T-cell epitopes on HtrA protein of Orientia tsutsugamushi","volume":"120","author":"Sankar","year":"2019"},{"key":"b12","series-title":"Dis Markers","first-page":"9130719","article-title":"In silico identification of new targets for diagnosis, vaccine, and drug candidates against Trypanosoma cruzi","volume":"2020","author":"Trevisan","year":"2020"},{"key":"b13","series-title":"VacciMonitor","first-page":"43","article-title":"Comparaci\u00f3n de la antigenicidad de dos construcciones pept\u00eddicas de mimotopos del virus de la hepatitis A mediante suero de ratones inmunizados","volume":"22","author":"Aguilar","year":"2013"},{"key":"b14","series-title":"J Vector Borne Dis","first-page":"317","article-title":"Immunogenicity of OmpA and OmpB antigens from Rickettsia rickettsii on mononuclear cells from Rickettsia positive Mexican patients","volume":"54","author":"Dzul-Rosado","year":"2017"},{"key":"b15","series-title":"Pathog Dis","first-page":"ftv101","article-title":"Failure of a heterologous recombinant Sca5\/OmpB protein-based vaccine to elicit effective protective immunity against Rickettsia rickettsii infections in C3H\/HeN mice","volume":"73","author":"Riley","year":"2015"},{"key":"b16","series-title":"Microbiol Immunol","first-page":"368","article-title":"Development of recombinant OmpA and OmpB proteins as diagnostic antigens for rickettsial disease","volume":"53","author":"Eun-Ju","year":"2009"},{"key":"b17","series-title":"Rev Univ Ind Santander Salud","first-page":"243","article-title":"Fiebre manchada por Rickettsia rickettsii en las Am\u00e9ricas: Un problema crecientsalud p\u00fablica","volume":"47","author":"\u00c1lvarez-Hern\u00e1ndez","year":"2015"},{"key":"b18","series-title":"Hum Immunol","first-page":"569","article-title":"Genetic diversity of HLA system in two populations from Yucat\u00e1n, Mexico: M\u00e9rida and rural Yucat\u00e1n","volume":"81","author":"Lara-Riegos","year":"2020"},{"key":"b19","series-title":"mBio","first-page":"e00095-18","article-title":"Diagnosis of Zika virus infection by peptide array and enzyme-linked immunosorbent assay","volume":"9","author":"Mishra","year":"2018"},{"key":"b20","series-title":"Trans R Soc Trop Med Hyg","first-page":"653","article-title":"A review of dengue diagnostics and implications for surveillance and control","volume":"113","author":"Raafat","year":"2019"},{"key":"b21","series-title":"Rev Biomed","first-page":"73","article-title":"Importancia de las prote\u00ednas OmpA y OmpB en el desarrollo de vacunas contra la rickettsiosis","volume":"30","author":"Martinez-Miranda","year":"2019"},{"key":"b22","series-title":"Microbes Infect","first-page":"711","article-title":"Antibody response to dengue virus","volume":"16","author":"Cedillo-Barr\u00f3n","year":"2014"},{"key":"b23","series-title":"J Biomol Struct Dyn","first-page":"2546","article-title":"Exploring dengue proteome to design an effective epitope-based vaccine against dengue virus","volume":"37","author":"Sabetian","year":"2019"},{"key":"b24","series-title":"Front Microbiol","first-page":"1107496","article-title":"Structural and immunological basis of cross-reactivity between dengue and Zika infections: Implications in serosurveillance in endemic regions","volume":"14","author":"Gaspar-Castillo","year":"2023"},{"key":"b25","series-title":"PLoS One","first-page":"e100253","article-title":"Identification of novel surface-exposed proteins of Rickettsia rickettsii by affinity purification and proteomics","volume":"9","author":"Gong","year":"2014"},{"key":"b26","series-title":"Inmunology","first-page":"319","article-title":"MHC class II epitope predictive algorithms","volume":"130","author":"Nielsen","year":"2010"},{"key":"b27","series-title":"BMC Bioinformatics","first-page":"S13","article-title":"Prediction of peptides binding to MHC class I and II alleles by temporal motif mining","volume":"14","author":"Meydan","year":"2013"},{"year":"2014","author":"Owen","series-title":"KUBY Inmunolog\u00eda, S\u00e9ptima Edici\u00f3n","key":"b28"},{"key":"b29","series-title":"Acta Bioqu\u00edm Cl\u00edn Latinoam","first-page":"505","article-title":"Control de Calidad en pruebas cualitativas aplicado a pruebas serol\u00f3gicas","volume":"53","author":"Aberer","year":"2019"},{"key":"b30","series-title":"Am J Vet Res","first-page":"71","article-title":"Comparison of Anaplasma and Ehrlichia species-specific peptide ELISAs with whole organism-based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs","volume":"82","author":"Qurollo","year":"2021"},{"key":"b31","series-title":"J Clin Microbiol","first-page":"1581","article-title":"Use of peptide-based enzyme-linked immunosorbent assay followed by immunofluorescence assay to document Ehrlichia chaffeensis as a cause of febrile illness in Nicaragua","volume":"54","author":"Chikeka","year":"2016"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/112\/4\/article-p909.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/112\/4\/article-p909.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/112\/4\/article-p909.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T13:51:28Z","timestamp":1743601888000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/112\/4\/article-p909.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,2]]},"references-count":31,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.24-0491","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2025,4,2]]}}}