{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T04:10:39Z","timestamp":1746677439526,"version":"3.40.5"},"reference-count":17,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Rotavirus (RV) is a leading cause of severe gastroenteritis in infants and young children, often resulting in dehydration and hospitalization. Although global data on RV is well-documented, there is limited information on its prevalence and genetic diversity in Bihar, India. This study aimed to investigate the prevalence of RV infections in the patient, from January 2021 to June 2024, and assess the associated molecular and epidemiological patterns. In this retrospective study conducted at the Rajendra Memorial Research Institute of Medical Sciences, Patna, 1,820 stool samples from patients suspected of RV infection were collected. Enzyme immunoassays were used to detect RV antigens, and positive samples were confirmed by real-time polymerase chain reaction targeting the VP4, VP6, and VP7 genes. Phylogenetic analysis was performed to examine genetic diversity. Results showed a 10% positivity rate for RV, with 5% showing equivocal results. The highest prevalence was in the 6\u201311 years age group (72 positive cases), followed by the 0\u20135 years group (62 positive cases). Prevalence decreased in older age groups, suggesting immunity through natural infection or vaccination. Phylogenetic analysis revealed distinct regional clusters and genetic variability between strains from Bihar and other parts of India, such as New Delhi and Kolkata. This study provides valuable baseline data on RV prevalence and genetic diversity in Bihar, emphasizing the need for vaccination and surveillance, particularly for younger children at higher risk. The observed genetic diversity suggests regional variations, highlighting the importance of continuous surveillance across India.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0543","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T21:41:33Z","timestamp":1740519693000},"page":"1040-1043","source":"Crossref","is-referenced-by-count":0,"title":["Rotavirus Infection in Children Suffering from Diarrhea in Patna, Bihar Region, India: A Hospital-Based Study"],"prefix":"10.4269","volume":"112","author":[{"given":"Neetesh","family":"Jindal","sequence":"first","affiliation":[{"name":"Department of Virology, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Virology, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India;"},{"name":"Department of Biochemistry, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashiq Hussain","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Major","family":"Madhukar","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshan Kamal","family":"Topno","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganesh Chandra","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Department of Virology, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"J Pure Appl Microbiol","first-page":"471","article-title":"The current epidemiology of rotavirus infection in children less than 5 years of age after introduction of RV vaccine in India","volume":"16","author":"Kumar","year":"2022"},{"key":"b2","series-title":"J Clin Virol","first-page":"259","article-title":"Rotavirus genotyping: Keeping up with an evolving population of human rotaviruses","volume":"31","author":"Iturriza-G\u00f3mara","year":"2004"},{"key":"b3","series-title":"Rev Med Virol","first-page":"29","article-title":"Global distribution 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transcription-PCR","volume":"46","author":"Lin","year":"2008"}],"container-title":["The American Journal of Tropical Medicine and 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