{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:14:12Z","timestamp":1781180052642,"version":"3.54.1"},"reference-count":59,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p><jats:italic>Campylobacter<\/jats:italic> enteritis is the most common bacterial foodborne disease in humans. Long-term use of antibiotics in chicken production may result in antimicrobial resistance in <jats:italic>Campylobacter<\/jats:italic> strains. Information on the antimicrobial resistance profile of <jats:italic>Campylobacter<\/jats:italic> species among free-range chickens in Ethiopia is scarce. Hence, this study aimed to determine the prevalence and antimicrobial resistance of <jats:italic>Campylobacter jejuni<\/jats:italic> and <jats:italic>Campylobacter coli<\/jats:italic> among free-range chickens in Amhara National Regional state, northwest Ethiopia from November 1, 2022 to April 30, 2023. Cloacal swabs were collected from free-range backyard chickens, directly inoculated onto modified charcoal cefoperazone deoxycholate agar, and incubated at reduced O<jats:sub>2<\/jats:sub> concentration at 42\u00b0C for 48 hours. Suspected colonies were confirmed at the species level using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The associated factors were analyzed using the Fisher exact test. A <jats:italic>P<\/jats:italic> &lt;0.05 at 95% CI was considered statistically significant. Among the 286 cloacal samples, 15.0% (<jats:italic>n<\/jats:italic> = 43\/286; CI: 10.2\u201319.5) were positive for <jats:italic>Campylobacter<\/jats:italic> species. <jats:italic>C. jejuni<\/jats:italic> (60.5%) was more frequent than <jats:italic>C. coli<\/jats:italic> (39.5%). Of the total isolates, 62.8% (<jats:italic>n<\/jats:italic> = 27\/43), 51.2% (<jats:italic>n<\/jats:italic> = 22\/43), and 16.3% (<jats:italic>n<\/jats:italic> = 7\/43) of the <jats:italic>Campylobacter<\/jats:italic> species were resistant to tetracycline, ciprofloxacin, and erythromycin, respectively. Of the total <jats:italic>Campylobacter<\/jats:italic> species isolates, 9.3% (<jats:italic>n<\/jats:italic> = 4\/43) were multidrug resistant. <jats:italic>Campylobacter<\/jats:italic> species resistance to tetracycline and ciprofloxacin was high in general among backyard chickens. Multidrug-resistant <jats:italic>Campylobacter<\/jats:italic> species were also identified, and they require special attention to prevent the potential dissemination of the strains to humans in the community.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0578","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T11:34:49Z","timestamp":1750764889000},"page":"694-700","source":"Crossref","is-referenced-by-count":1,"title":["Prevalence and Antimicrobial-Resistant Campylobacter jejuni and Campylobacter coli in Free-Range Chickens in Northwest Ethiopia"],"prefix":"10.4269","volume":"113","author":[{"given":"Mesfin","family":"Worku","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belay","family":"Tessema","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia;"},{"name":"Institute of Clinical Immunology, Faculty of Medicine, University of Leipzig, Leipzig, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Getachew","family":"Ferede","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linnet","family":"Ochieng","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi, Kenya;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubisa","family":"Abera Leliso","sequence":"additional","affiliation":[{"name":"Animal Health Institute, Addis Ababa, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Mutua","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi, Kenya;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshnee","family":"Moodley","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi, Kenya;"},{"name":"Department of Veterinary and Animal Science, Faculty of Health and Medical Science, University of Copenhagen, Frederiksberg, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baye","family":"Gelaw","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delia","family":"Grace","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi, Kenya;"},{"name":"Natural Resource Institute, University of Greenwich, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","article-title":"WHO Estimates of the Global Burden of Foodborne Diseases: Foodborne Disease Burden Epidemiology Reference Group 2007\u20132015","year":"2015"},{"key":"b2","series-title":"Pacific Int J","first-page":"105","article-title":"Review on village\/backyard\/poultry production system in Ethiopia","volume":"1","author":"Chaimiso","year":"2018"},{"key":"b3","article-title":"Central Statistical Authority. 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