{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:07:25Z","timestamp":1788217645286,"version":"build-2803163510"},"reference-count":31,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Rapidly identifying <jats:italic>Anopheles-<\/jats:italic>carrying malaria parasites is crucial for imported malaria prevention. However, suitable methods still lack quick detection in limited-resource situations. In this study, disc microfluidic isothermal amplification integrating loop-mediated isothermal amplification (LAMP) and microfluidic chip technology were applied to develop rapid and precise detection with low resource requirements. Primer set EMP1G2, which is specific to <jats:italic>Plasmodium falciparum<\/jats:italic> (<jats:italic>P. falciparum<\/jats:italic>) erythrocyte membrane protein 1, and primer set 18sG2, which is specific to ribosomal 18s subunit RNA, were screened for optimal LAMP-specific primer sets. The minimum detection limits were 125 copies\/\u00b5L for the EMP1G2 and 6,562 copies\/\u00b5L for the 18sG2. Subsequently, optimal primer sets were evaluated for specificity with nucleic acid from other mosquito-borne pathogens and arthropod vectors. No nonspecific amplification was observed in optimal amplification-specific primer sets with the DNA of <jats:italic>Anopheles<\/jats:italic> mosquitoes and morphologically similar arthropods or with the copy DNA of Zika virus, yellow fever virus, or dengue virus 1. The detection method was evaluated in a simulated scenario and demonstrated a robust capacity for rapid on-site detection. Additionally, polymerase chain reaction (PCR) and quantitative real-time PCR methods were compared using this method. In this study, a rapid detection method based on disc microfluidic isothermal amplification was developed that could be used to detect <jats:italic>P. falciparum<\/jats:italic> carried by mosquitoes in a field setting under limited resource conditions.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0599","type":"journal-article","created":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T14:50:06Z","timestamp":1735743006000},"page":"784-792","source":"Crossref","is-referenced-by-count":1,"title":["A Rapid On-Site Detection Method for Plasmodium falciparum Carried by Mosquitoes Using Disc Microfluidic Isothermal Amplification"],"prefix":"10.4269","volume":"112","author":[{"given":"Ning","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Pathogenic Biology, Basic Medical College, Naval Medical University, Shanghai, China;"},{"name":"Clinical Laboratory, Naval Medical Centre, Naval Medical University, Shanghai, 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