{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:11:42Z","timestamp":1785179502044,"version":"3.55.0"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,6]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>The dengue virus (DENV) and chikungunya virus (CHIKV) infection present with similar symptoms and are transmitted by <jats:italic>Aedes<\/jats:italic> mosquitoes. Until now, there have been no reported cases of DENV and CHIKV being detected in mosquitoes in Nepal. This study identified circulating arboviruses from mosquitoes collected in the capital city Kathmandu, and Dhading, Bhaktapur, and Lalitpur districts of Central Nepal in 2023. Of 105 <jats:italic>Aedes<\/jats:italic> pooled samples, 16 (15.2%) were positive for DENV, and six (5.7%) were positive for CHIKV by quantitative real-time polymerase chain reaction. DENV-2 was the most common serotype (11.4%), aligning with recent outbreaks in Nepal in 2023, followed by DENV-3 and DENV-4 (1.9% each). To the best of our knowledge, our study is the first report to detect DENV and CHIKV in <jats:italic>Aedes<\/jats:italic> mosquitoes circulating in Nepal. This study highlights the need for mosquito surveillance to prevent future outbreaks of mosquito-borne diseases in Nepal.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0685","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T13:25:58Z","timestamp":1748352358000},"page":"378-381","source":"Crossref","is-referenced-by-count":2,"title":["Detection of Dengue and Chikungunya Viruses in Mosquitoes from Nepal"],"prefix":"10.4269","volume":"113","author":[{"given":"Maurine Mumo","family":"Mutua","sequence":"first","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"},{"name":"Graduate School of Health, Kenya Medical Research Institute, Nairobi, Kenya;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandesh","family":"Rimal","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabin","family":"Shrestha","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjana","family":"Kharbuja","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Dulal","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suprabha","family":"Subedi","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Bhandari Dumre","sequence":"additional","affiliation":[{"name":"Freelance Public Health Professional, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashma","family":"Khadka","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishan","family":"Gautam","sequence":"additional","affiliation":[{"name":"Natural History Museum, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himal","family":"Luitel","sequence":"additional","affiliation":[{"name":"Agriculture and Forestry University, Chitwan, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merveille","family":"Kapandji","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Inoue","sequence":"additional","affiliation":[{"name":"Kenya Research Station, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Takamatsu","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basu Dev","family":"Pandey","sequence":"additional","affiliation":[{"name":"DEJIMA Infectious Diseases Research Alliance, Nagasaki University, Nagasaki, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Kenya Research Station, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Urano","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouichi","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"},{"name":"DEJIMA Infectious Diseases Research Alliance, Nagasaki University, Nagasaki, Japan;"},{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo, Japan;"},{"name":"Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam Prakash","family":"Dumre","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mya Myat","family":"Ngwe Tun","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;"},{"name":"DEJIMA Infectious Diseases Research Alliance, Nagasaki University, Nagasaki, Japan;"},{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo, Japan;"},{"name":"Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Emerg Microbes Infect","first-page":"e18","article-title":"Factors responsible for the emergence of arboviruses; strategies, challenges, and limitations for their 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viruses reveals modulation of the replication of both viruses in midguts and salivary glands of Aedes aegypti mosquitoes","volume":"18","author":"Le Coupanec","year":"2017"},{"key":"b15","series-title":"Pak J Zool","first-page":"273","article-title":"Role of Aedes and culex in dissemination of dengue virus","volume":"47","author":"Shah","year":"2015"},{"key":"b16","series-title":"Virology","first-page":"96","article-title":"Surveillance of Zika and Dengue viruses in field-collected Aedes aegypti mosquitoes from different states of India","volume":"574","author":"Akhtar","year":"2022"}],"container-title":["The American Journal of Tropical Medicine and 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