{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T13:10:44Z","timestamp":1759497044312,"version":"3.41.0"},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Since first isolate of <jats:italic>Candida <\/jats:italic>(<jats:italic>C.<\/jats:italic>) <jats:italic>auris<\/jats:italic> was recognized in 2018 in China, over 60 cases of <jats:italic>C. auris<\/jats:italic> colonization or infection have been reported up to 2022, with at least two distinct genetic clade. Most isolates from South Asian clade and South African clade were sensitive and resistant to fluconazole, respectively. Here, we report an isolate of fluconazole-resistant South Asian clade <jats:italic>C. auris<\/jats:italic> (PUSH159) from blood. The efflux pump genes <jats:italic>MDR1<\/jats:italic>, <jats:italic>CDR1<\/jats:italic>, <jats:italic>CDR4<\/jats:italic>, <jats:italic>SNQ2,<\/jats:italic> and <jats:italic>ARB1<\/jats:italic>, and ergosterol biosynthesis gene <jats:italic>ERG11<\/jats:italic> were detected in strain PUSH159. Single nucleotide polymorphism (SNP) analysis revealed that strain PUSH159 carried multiple mutations in drug resistance-associated genes, including a hot-spot mutation (Y132F) in <jats:italic>ERG11<\/jats:italic>, missense mutation of E709D in <jats:italic>CDR1<\/jats:italic>, and missense mutations of K52N and E464K in <jats:italic>SNQ2. <\/jats:italic>Our findings support the genetic diversity and differences in antifungal susceptibility within the South Asian clade, and provide early warning for the emergence of fluconazole-resistant South Asian clade isolates in China.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0748","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T17:39:51Z","timestamp":1745343591000},"page":"184-186","source":"Crossref","is-referenced-by-count":1,"title":["Isolation of Fluconazole-Resistant South Asian Clade Candida auris in Beijing, China"],"prefix":"10.4269","volume":"113","author":[{"given":"Linlin","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoukui","family":"Hu","sequence":"additional","affiliation":[{"name":"Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Clin Infect Dis","first-page":"134","article-title":"Simultaneous emergence of multidrug-resistant Candida auris on 3 continents confirmed by whole-genome sequencing and epidemiological analyses","volume":"64","author":"Lockhart","year":"2017"},{"key":"b2","series-title":"Clin Infect Dis","first-page":"141","article-title":"Emergence of Candida auris: An international call to arms","volume":"64","author":"Clancy","year":"2017"},{"year":"2019","series-title":"Antimicrobial Resistance: Antimicrobial Resistance & Death Estimates: Antibiotic Resistance Threats in the United States","key":"b3"},{"key":"b4","series-title":"Emerg Microbes Infect","first-page":"93","article-title":"The first isolate of Candida auris in China: Clinical and biological aspects","volume":"7","author":"Wang","year":"2018"},{"key":"b5","series-title":"J Infect","first-page":"561","article-title":"Emergency of fungemia cases caused by fluconazole-resistant Candida auris in Beijing, China","volume":"77","author":"Chen","year":"2018"},{"key":"b6","series-title":"Virulence","first-page":"1388","article-title":"A biological and genomic comparison of a drug-resistant and a drug-susceptible strain of Candida auris isolated from Beijing, China","volume":"12","author":"Fan","year":"2021"},{"key":"b7","series-title":"Emerg Microbes Infect","first-page":"1088","article-title":"Genomic epidemiology of Candida auris in a general hospital in Shenyang, China: A 3-year surveillance study","volume":"10","author":"Tian","year":"2021"},{"key":"b8","series-title":"Microbiol Resour Announc","first-page":"e00308-20","article-title":"Draft genome sequences of 19 clinical isolates of Candida auris from Hong Kong","volume":"10","author":"Tse","year":"2021"},{"key":"b9","series-title":"Virulence","first-page":"589","article-title":"Candida auris infections in China","volume":"13","author":"Du","year":"2022"},{"year":"2020","series-title":"Candida auris: Laboratorians and Health Professionals: Antifungal Susceptibility Testing and Interpretation","key":"b10"},{"key":"b11","series-title":"J Antimicrob Chemother","first-page":"1794","article-title":"Candida auris candidaemia in Indian ICUs: Analysis of risk factors","volume":"72","author":"Rudramurthy","year":"2017"},{"key":"b12","series-title":"Emerg Microbes Infect","first-page":"1867","article-title":"The first invasive Candida auris infection in Taiwan","volume":"11","author":"Tsai","year":"2022"},{"key":"b13","series-title":"Antimicrob Agents Chemother","first-page":"e00057-19","article-title":"Abrogation of triazole resistance upon deletion of CDR1 in a clinical isolate of Candida auris","volume":"63","author":"Rybak","year":"2019"},{"key":"b14","series-title":"Nat Commun","first-page":"5346","article-title":"Genomic insights into multidrug-resistance, mating and virulence in Candida auris and related emerging species","volume":"9","author":"Mu\u00f1oz","year":"2018"},{"key":"b15","series-title":"Front Microbiol","first-page":"708009","article-title":"A comparative transcriptome between anti-drug sensitive and resistant Candida auris in China","volume":"12","author":"Zhou","year":"2021"},{"key":"b16","series-title":"Clin Microbiol Infect","first-page":"792","article-title":"Resistance of Candida to azoles and echinocandins worldwide","volume":"25","author":"Pristov","year":"2019"},{"key":"b17","series-title":"Antimicrob Agents Chemother","first-page":"e01427-18","article-title":"Limited ERG11 mutations identified in isolates of Candida auris directly contribute to reduced azole susceptibility","volume":"62","author":"Healey","year":"2018"},{"key":"b18","series-title":"mBio","first-page":"e02529-18","article-title":"Genetic analysis of Candida auris implicates Hsp90 in morphogenesis and azole tolerance and Cdr1 in azole resistance","volume":"10","author":"Kim","year":"2019"},{"key":"b19","series-title":"mBio","first-page":"e03333-20","article-title":"Genome-wide analysis of experimentally evolved Candida auris reveals multiple novel mechanisms of multidrug resistance","volume":"12","author":"Carolus","year":"2021"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/113\/1\/article-p184.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/113\/1\/article-p184.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/113\/1\/article-p184.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T13:55:03Z","timestamp":1751464503000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/113\/1\/article-p184.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":19,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.24-0748","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2025,7,2]]}}}