{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:52:31Z","timestamp":1769075551595,"version":"3.49.0"},"reference-count":32,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Fascioliasis, which is caused by <jats:italic>Fasciola hepatica<\/jats:italic> and <jats:italic>Fasciola gigantica<\/jats:italic> (<jats:italic>F. gigantica<\/jats:italic>), is a public health problem worldwide. Stages infective to humans occur on aquatic plants and in water. The gold standard for diagnosing human infection depends on finding fluke eggs in stool samples. However, this method has low diagnostic sensitivity; eggs from other trematode species might be misidentified as those of <jats:italic>Fasciola<\/jats:italic>, and eggs are not voided in ectopic infections. Therefore, serological analysis can support the diagnosis of human fascioliasis. We evaluated whether a new fascioliasis immunochromatographic test kit, which detects specific IgG antibodies using <jats:italic>F. gigantica<\/jats:italic> excretory\u2013secretory antigen, can be used for rapid diagnosis from whole blood. The kit (\u201cthe fascioliasis whole-blood test kit\u201d) was evaluated under laboratory conditions using 250 whole-blood samples (WBSs), of which 41 were from fascioliasis cases. Results based on simulated WBSs and the corresponding serum samples were compared and showed almost perfect agreement (percent agreement = 97.2%; kappa value = 0.9145). The diagnostic sensitivity and specificity of the test using simulated WBSs were 97.6% and 96.2%, respectively. Comparable values when serum samples were tested were 100.0% and 93.3%, respectively. This kit represents a significant advance because it does not require extensive training of personnel, is easy to use, and can support diagnosis at the bedside or in local and remote hospitals with limited facilities. The kit may also contribute significantly to epidemiological surveys.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0754","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T16:23:40Z","timestamp":1739291020000},"page":"1000-1005","source":"Crossref","is-referenced-by-count":3,"title":["Lateral-Flow Antibody Detection Device for the Diagnosis of Human Fascioliasis Using Simulated Whole-Blood Samples"],"prefix":"10.4269","volume":"112","author":[{"given":"Patcharaporn","family":"Boonroumkaew","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakkhana","family":"Sadaow","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nongnapas","family":"Kanchanangkul","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan;"},{"name":"Department of Tropical Medicine and Parasitology, School of Medicine, Juntendo University, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Clin Microbiol Rev","first-page":"e0008819","article-title":"Human and animal fascioliasis: Origins and worldwide evolving scenario","volume":"35","author":"Mas-Coma","year":"2022"},{"key":"b2","series-title":"J Helminthol","first-page":"511","article-title":"Current status of fasciolosis in Vietnam: An update and perspectives","volume":"90","author":"Bui","year":"2016"},{"key":"b3","series-title":"Waterborne Zoonoses: Identification, Causes, and Control","first-page":"305","author":"Mas-Coma","year":"2004"},{"key":"b4","series-title":"Biology of Food Borne Parasites","first-page":"371","author":"Mas-Coma","year":"2015"},{"key":"b5","series-title":"Parasite Immunol","first-page":"558","article-title":"A prospective view of animal and human fasciolosis","volume":"38","author":"Cwiklinski","year":"2016"},{"key":"b6","series-title":"J Helminthol","first-page":"e12","article-title":"Advances in diagnostic approaches to Fasciola infection in animals and humans: An overview","volume":"98","author":"Aftab","year":"2024"},{"key":"b7","series-title":"Jpn J Parasitol","first-page":"373","article-title":"A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis","volume":"38","author":"Yamasaki","year":"1989"},{"key":"b8","series-title":"Diagn Microbiol Infect Dis","first-page":"175","article-title":"Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L\u2212ELISA","volume":"44","author":"Rokni","year":"2002"},{"key":"b9","series-title":"Am J Trop Med Hyg","first-page":"82","article-title":"Serodiagnosis of human fascioliasis by a cystatin capture enzyme\u2212linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen","volume":"72","author":"Tantrawatpan","year":"2005"},{"key":"b10","series-title":"PLoS Negl Trop Dis","first-page":"e2414","article-title":"The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease","volume":"7","author":"Gonzales Santana","year":"2013"},{"key":"b11","series-title":"Exp Parasitol","first-page":"76","article-title":"Design and synthesis of a new peptide derived from Fasciola gigantica cathepsin L1 with potential application in serodiagnosis of fascioliasis","volume":"189","author":"Meshgi","year":"2018"},{"key":"b12","series-title":"PLoS Negl Trop Dis","first-page":"e1812","article-title":"Field evaluation of a coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries","volume":"6","author":"Valero","year":"2012"},{"key":"b13","series-title":"PLoS One","first-page":"e0145650","article-title":"Immunodiagnosis of Fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and immunochromatographic assay for detection of circulating cathepsin L1 protease","volume":"11","author":"Anuracpreeda","year":"2016"},{"key":"b14","series-title":"PLoS Negl Trop Dis","first-page":"e1376","article-title":"Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis","volume":"5","author":"Mart\u00ednez-Sern\u00e1ndez","year":"2011"},{"key":"b15","series-title":"Parasitol Res","first-page":"3691","article-title":"Effectiveness of Fasciola gigantica excretory\u2212secretory and recombinant cathepsin L antigens for rapid diagnosis of human fascioliasis using immunochromatographic devices","volume":"119","author":"Sadaow","year":"2020"},{"key":"b16","series-title":"Am J Trop Med Hyg","first-page":"648","article-title":"Fasciola gigantica-specific antigens: Purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis","volume":"61","author":"Maleewong","year":"1999"},{"key":"b17","series-title":"Progress of Medical Parasitology in Japan","first-page":"359","author":"Watanabe","year":"1965"},{"key":"b18","series-title":"J Parasitol","first-page":"712","article-title":"Animal fascioliasis in Khuzestan, southwestern Iran","volume":"58","author":"Sahba","year":"1972"},{"key":"b19","series-title":"J Biol Chem","first-page":"265","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951"},{"key":"b20","series-title":"Southeast Asian J Trop Med Public Health","first-page":"579","article-title":"Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera","volume":"29","author":"Intapan","year":"1998"},{"key":"b21","series-title":"Trans R Soc Trop Med Hyg","first-page":"774","article-title":"The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection","volume":"80","author":"Elkins","year":"1986"},{"key":"b22","series-title":"Am J Trop Med Hyg","first-page":"389","article-title":"Nine human sparganosis cases in Thailand with molecular identification of causative parasite species","volume":"91","author":"Boonyasiri","year":"2014"},{"key":"b23","series-title":"Parasit Vectors","first-page":"14","article-title":"Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis","volume":"9","author":"Janwan","year":"2016"},{"key":"b24","series-title":"Trop Med Parasitol","first-page":"167","article-title":"Persistence of IgG, IgM, and IgE antibodies in human trichinosis","volume":"43","author":"Morakote","year":"1992"},{"key":"b25","series-title":"Asian Pac J Allergy Immunol","first-page":"267","article-title":"Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis","volume":"19","author":"Maleewong","year":"2001"},{"key":"b26","series-title":"Southeast Asian J Trop Med Public Health","first-page":"176","article-title":"Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis","volume":"25","author":"Wongkham","year":"1994"},{"key":"b27","series-title":"Am J Trop Med Hyg","first-page":"518","article-title":"A modified agar plate method for detection of Strongyloides stercoralis","volume":"45","author":"Koga","year":"1991"},{"key":"b28","series-title":"Asian Pac J Allergy Immunol","first-page":"237","article-title":"Evaluation of IgG4 and total IgG antibodies against cysticerci and peptide antigens for the diagnosis of human neurocysticercosis by ELISA","volume":"26","author":"Intapan","year":"2008"},{"key":"b29","series-title":"Rev Inst Med Trop Sao Paulo","first-page":"397","article-title":"A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni","volume":"14","author":"Katz","year":"1972"},{"key":"b30","series-title":"Clinical Parasitology","first-page":"733","author":"Beaver","year":"1984"},{"key":"b31","series-title":"Diagnostics (Basel)","first-page":"862","article-title":"Development of immunochromatographic test kit for rapid detection of specific IgG4 antibody in whole-blood samples for diagnosis of human gnathostomiasis","volume":"11","author":"Janwan","year":"2021"},{"key":"b32","series-title":"Clin Microbiol Infect","first-page":"1265","article-title":"Host genetics and parasitic infections","volume":"20","author":"Mangano","year":"2014"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/112\/5\/article-p1000.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/112\/5\/article-p1000.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/112\/5\/article-p1000.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T14:24:15Z","timestamp":1746627855000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/112\/5\/article-p1000.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,7]]},"references-count":32,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.24-0754","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,7]]}}}