{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T14:05:52Z","timestamp":1781273152073,"version":"3.54.1"},"reference-count":69,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Ticks are blood-feeding arthropods and serve as vectors and reservoirs for diverse pathogens. Recent environmental changes have triggered the invasion of ticks into new geographical areas, prompting a public health alert for increased risk of tick-borne diseases. <jats:italic>Amblyomma<\/jats:italic> (<jats:italic>A.<\/jats:italic>) <jats:italic>americanum<\/jats:italic> (lone star tick) has emerged as the most common human-biting tick species in the eastern United States. <jats:italic>Amblyomma americanum<\/jats:italic> transmits multiple pathogens, including <jats:italic>Rickettsia<\/jats:italic> (<jats:italic>R.<\/jats:italic>) <jats:italic>amblyommatis<\/jats:italic>, the suspected cause of mild spotted fever rickettsiosis. As an invasive tick species, <jats:italic>Haemaphysalis<\/jats:italic> (<jats:italic>H.<\/jats:italic>) <jats:italic>longicornis<\/jats:italic> (longhorned tick) has rapidly invaded and expanded to high densities in the eastern United States. <jats:italic>Haemaphysalis longicornis<\/jats:italic> and <jats:italic>A. americanum<\/jats:italic> often share the habitat with preferential feeding on mid- to large-sized animals, such as white-tailed deer. This sympatric association of <jats:italic>H. longicornis<\/jats:italic> with <jats:italic>A. americanum<\/jats:italic> raises the potential for <jats:italic>H. longicornis<\/jats:italic> to acquire pathogens native to <jats:italic>A. americanum<\/jats:italic> during blood-feeding on the same host. In its native ranges, <jats:italic>H. longicornis<\/jats:italic> transmits <jats:italic>R. japonica<\/jats:italic> and <jats:italic>R. heilongjiangensis<\/jats:italic>. However, it remains unclear whether <jats:italic>H. longicornis<\/jats:italic> can transmit <jats:italic>R. amblyommatis<\/jats:italic> abundantly present in <jats:italic>A. americanum<\/jats:italic> in the United States. Using artificial membrane feeding, we establish that <jats:italic>R. amblyommatis<\/jats:italic> can stably colonize <jats:italic>H. longicornis<\/jats:italic> without altering feeding and molting behaviors. Transovarial transmission of <jats:italic>R. amblyommatis<\/jats:italic> did not occur in parthenogenetic <jats:italic>H. longicornis.<\/jats:italic> However, <jats:italic>R. amblyommatis<\/jats:italic> successfully invaded the midgut and salivary glands of <jats:italic>H. longicornis<\/jats:italic>, key organ tissues of rickettsial replication and horizontal transmission. Our results suggest that <jats:italic>H. longicornis<\/jats:italic> may serve as a vector, but not as a reservoir, for <jats:italic>R. amblyommatis<\/jats:italic> transmission.<\/jats:p>","DOI":"10.4269\/ajtmh.24-0782","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T17:39:09Z","timestamp":1745948349000},"page":"147-155","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of Transstadial Transmission of Rickettsia Amblyommatis in Haemaphysalis Longicornis Using Optimized Artificial Membrane Feeding System"],"prefix":"10.4269","volume":"113","author":[{"given":"Justin","family":"Le","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly B.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Yi","family":"Yen","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smruti","family":"Mishra","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Carlson","sequence":"additional","affiliation":[{"name":"Institute for Advanced Computational Science, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Handel","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Stony Brook University, Stony Brook, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich R.","family":"Mackow","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge L.","family":"Benach","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilia","family":"Rochlin","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwan Keun","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York;"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"J Infect Dis","first-page":"370","article-title":"Range expansion of native and invasive ticks: A looming public health 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