{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:56Z","timestamp":1787031176106,"version":"build-2736575974"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,6]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n                  <jats:p>Dengue fever, a vector-borne disease caused by four serotypes of dengue virus (DENV), ranges from asymptomatic to severe illness, including hemorrhagic fever and shock syndrome. A 2023 outbreak (August 2023\u2013May 2024) in Mali affected six districts in Bamako, causing 1,422 confirmed cases with a 2.7% fatality rate. In this study, viral sequencing provided insights into the molecular epidemiology and transmission dynamics of this outbreak. From September to October 2023, 23 of 42 suspected cases detected through national dengue surveillance activities were tested using the pan-vertebrate virus metagenomics method (virome capture sequencing platform for vertebrate viruses [VirCapSeq-VERT]). Sequencing data were analyzed using the Rapid Identification of Microbes pipeline, and Bayesian phylogenetic inference with Monte Carlo methods was used to assess viral genomic evolution. Among 23 patients, 61% were male, and the median age was 37 years (range: 20\u201374). The most common symptoms were fever (93.1%), headache (56.5%), and asthenia (47.8%). The largest proportion experienced dengue with warning signs (65.2%), followed by dengue without warning signs (30.4%) and severe dengue (4.4%). The successful sequencing of 19 samples revealed dengue virus serotype 3 (DENV-3; genotype III) in 15 (65.21%) samples and dengue virus serotype 1 (DENV-1; genotype III) in 4 (17.39%) samples. The DENV-1 sequences were analogous to West African sequences, and the DENV-3 were clustered with West African, Asian, and Caribbean sequences. In this study of DENV in Mali, we offer insights into the molecular epidemiology of the virus and underscore the benefits of the genomic surveillance of arboviruses in West Africa.<\/jats:p>","DOI":"10.4269\/ajtmh.25-0113","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T15:28:40Z","timestamp":1749569320000},"page":"374-377","source":"Crossref","is-referenced-by-count":2,"title":["Analyzing Transmission Patterns of Two Dengue Virus Serotypes during the 2023 Outbreak in Mali, West Africa"],"prefix":"10.4269","volume":"113","author":[{"given":"Fousseyni","family":"Kane","sequence":"first","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"},{"name":"African Centre of Excellence in Bioinformatics and Data Intensive Science (ACE-B), USTTB, Bamako, Mali;"},{"name":"Global Alliance for Preventing Pandemics, Columbia University, New York, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yacouba","family":"Cissoko","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Collins","sequence":"additional","affiliation":[{"name":"Global Alliance for Preventing Pandemics, Columbia University, New York, New York;"},{"name":"Center for Infection and Immunity, Columbia University, New York, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dramane","family":"Diallo","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"},{"name":"Global Alliance for Preventing Pandemics, Columbia University, New York, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bassirou","family":"Diarra","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou","family":"Kone","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"},{"name":"African Centre of Excellence in Bioinformatics and Data Intensive Science (ACE-B), USTTB, Bamako, Mali;"},{"name":"Global Alliance for Preventing Pandemics, Columbia University, New York, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djeneba K.","family":"Dabitao","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"},{"name":"African Centre of Excellence in Bioinformatics and Data Intensive Science (ACE-B), USTTB, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumar","family":"Sako","sequence":"additional","affiliation":[{"name":"African Centre of Excellence in Bioinformatics and Data Intensive Science (ACE-B), USTTB, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tenin A.","family":"Coulibaly","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daouda","family":"Keita","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antieme Combo Georges","family":"Togo","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gagni","family":"Coulibaly","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheick O.","family":"Tangara","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamadou","family":"Diakite","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"},{"name":"African Centre of Excellence 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Leah","family":"Katzelnick","sequence":"additional","affiliation":[{"name":"Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamila","family":"Aboulhab","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Akpa","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Fouth-Tchos","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron T.","family":"Neal","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Shaw-Saliba","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sounkalo","family":"Dao","sequence":"additional","affiliation":[{"name":"University Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray Y.","family":"Chen","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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Dis","first-page":"1584","article-title":"Viremia and clinical presentation in Nicaraguan patients infected with Zika virus, chikungunya virus, and dengue virus","volume":"63","author":"Waggoner","year":"2016"},{"key":"b3","series-title":"Nat Rev Microbiol","first-page":"S7","article-title":"Dengue: A continuing global threat","volume":"8","author":"Guzman","year":"2010"},{"key":"b4","series-title":"Science","first-page":"476","article-title":"Pathogenesis of dengue: Challenges to molecular biology","volume":"239","author":"Halstead","year":"1988"},{"key":"b5","series-title":"Lancet","first-page":"434","article-title":"Dengue epidemic in Burkina Faso: How can the response improve?","volume":"403","author":"Manigart","year":"2024"},{"key":"b6","series-title":"PLoS Negl Trop Dis","first-page":"e0009829","article-title":"Emergence of dengue virus serotype 2 in Mauritania and molecular characterization of its circulation in West Africa","volume":"15","author":"Fouri\u00e9","year":"2021"},{"key":"b7","series-title":"Vector Borne Zoonotic Dis","first-page":"1479","article-title":"Dengue virus seroprevalence among febrile patients in Bamako, Mali: Results of a 2006 surveillance study","volume":"11","author":"Phoutrides","year":"2011"},{"key":"b8","series-title":"Surveillance \u00c9pid\u00e9miologique de la Dengue au Centre D\u2019infectiologie Charles M\u00e9rieux de Janvier a D\u00e9cembre 2019","author":"Traore","year":"2021"},{"key":"b9","series-title":"Am J Trop Med Hyg","first-page":"107","article-title":"Seroprevalence of arboviruses in a malaria hyperendemic area in Southern Mali","volume":"111","author":"Bane","year":"2024"},{"key":"b10","series-title":"Dengue Outbreak Report","year":"2024"},{"key":"b11","series-title":"Virusdisease","first-page":"365","article-title":"Utility of CDC DENV1\u20134 real time PCR assay and trioplex assay for the diagnosis of dengue in patients with acute febrile illness","volume":"34","author":"Sarkar","year":"2023"},{"key":"b12","series-title":"mBio","first-page":"e01491","article-title":"Virome capture sequencing enables sensitive viral diagnosis and comprehensive virome analysis","volume":"6","author":"Briese","year":"2015"},{"key":"b13","series-title":"J Clin Microbiol","first-page":"e0061223","article-title":"Validation of the VirCapSeq-VERT system for differential diagnosis, detection, and surveillance of viral infections","volume":"62","author":"Kapoor","year":"2024"},{"key":"b14","article-title":"Dengue- Global Situation","year":"2023"},{"key":"b15","article-title":"Des Nouveaux Cas de Dengue Signal\u00e9s au Mali, \u00e0 Maurice et au S\u00e9n\u00e9gal","author":"Gerome","year":"2024"},{"key":"b16","doi-asserted-by":"crossref","DOI":"10.1101\/2024.10.22.24315827","article-title":"A Decade of Dengue Disease Burden in Africa (2013\u20132023): A Systematic Review","author":"Mwanyika","year":"2024"},{"key":"b17","series-title":"Exp Biol 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