{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T18:48:35Z","timestamp":1778525315426,"version":"3.51.4"},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Ethiopia faces an increase in <jats:italic>Plasmodium falciparum<\/jats:italic> resistant to rapid diagnostic test (RDT) detection based on histidine-rich proteins (HRPs) because of deletions of the encoding genes (<jats:italic>pfhrp2\/3<\/jats:italic>). Simultaneously, <jats:italic>P. falciparum Kelch13<\/jats:italic> (<jats:italic>pfk13<\/jats:italic>) artemisinin partial resistance markers, particularly R622I, are common in northern and western Ethiopia. We assessed <jats:italic>pfhrp2\/3<\/jats:italic>, <jats:italic>pfk13<\/jats:italic>, and <jats:italic>Plasmodium falciparum multidrug resistance 1<\/jats:italic> (<jats:italic>pfmdr1<\/jats:italic>) variants among 220 symptomatic malaria patients presenting at health facilities in central Ethiopia in 2023. <jats:italic>Plasmodium falciparum<\/jats:italic> infection was present in 86.4%, <jats:italic>Plasmodium vivax<\/jats:italic> infection was present in 12.3%, and <jats:italic>Plasmodium ovale<\/jats:italic> infection was present in 3.6% of collected isolates. Among <jats:italic>P. falciparum<\/jats:italic> infections, the validated artemisinin partial resistance marker <jats:italic>pfk13<\/jats:italic> R622I occurred in 1.1% (<jats:italic>n<\/jats:italic> = 2\/174), and candidate marker P441L occurred in 1.7% (<jats:italic>n<\/jats:italic> = 3\/174). <jats:italic>pfhrp2<\/jats:italic>, <jats:italic>pfhrp3<\/jats:italic>, and dual deletions were seen in 24.0% (<jats:italic>n<\/jats:italic> = 36\/150), 78.8% (<jats:italic>n<\/jats:italic> = 118\/150), and 22.7% (<jats:italic>n<\/jats:italic> = 34\/150), respectively. The <jats:italic>pfmdr1<\/jats:italic> pattern indicated reduced lumefantrine sensitivity. These data suggest that high proportions of <jats:italic>P. falciparum<\/jats:italic> infections are being missed by HRP-based RDTs, whereas <jats:italic>pfk13<\/jats:italic> mutations are relatively rare.<\/jats:p>","DOI":"10.4269\/ajtmh.25-0114","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T17:31:00Z","timestamp":1752600660000},"page":"620-622","source":"Crossref","is-referenced-by-count":2,"title":["Plasmodium falciparum Kelch13 Variants and Histidine-Rich Protein 2\/3 Deletions in Central Ethiopia, 2023"],"prefix":"10.4269","volume":"113","author":[{"given":"Tafese Beyene","family":"Tufa","sequence":"first","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"College of Health Sciences, Arsi University, Asella, Ethiopia;"},{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Center for Global Health, Institute of International Health, Charit\u00e9\u2013Universitaetsmedizin Berlin, Berlin, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Schallenberg","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Center for Global Health, Institute of International Health, Charit\u00e9\u2013Universitaetsmedizin Berlin, Berlin, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason A.","family":"Hendry","sequence":"additional","affiliation":[{"name":"Malaria Parasite Biology, Max Planck Institute for Infection Biology, Berlin, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clement","family":"Igiraneza","sequence":"additional","affiliation":[{"name":"University Teaching Hospital of Butare, Huye, Rwanda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Jara-Reznakova","sequence":"additional","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yannik","family":"Eggers","sequence":"additional","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helge","family":"Sill","sequence":"additional","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagaga","family":"Kenea","sequence":"additional","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"College of Health Sciences, Arsi University, Asella, Ethiopia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Luedde","sequence":"additional","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torsten","family":"Feldt","sequence":"additional","affiliation":[{"name":"Hirsch Institute of Tropical Medicine, Asella, Ethiopia;"},{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jules Minega","family":"Ndoli","sequence":"additional","affiliation":[{"name":"University Teaching Hospital of Butare, Huye, Rwanda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank P.","family":"Mockenhaupt","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Center for Global Health, Institute of International Health, Charit\u00e9\u2013Universitaetsmedizin Berlin, Berlin, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Welmoed","family":"van Loon","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Center for Global Health, Institute of International Health, Charit\u00e9\u2013Universitaetsmedizin Berlin, Berlin, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","article-title":"Disease Outbreak News. 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